PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08530 [old locus tag: SA1515 ]
• pan locus tag^?: SAUPAN004316000
• symbol: SA_RS08530
• pan gene symbol^?: phoR
• synonym:
• product: Alkaline phosphatase synthesis sensor protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08530 [old locus tag: SA1515 ]
• symbol: SA_RS08530
• product: Alkaline phosphatase synthesis sensor protein
• replicon: chromosome
• strand: -
• coordinates: 1727007..1728668
• length: 1662
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1727007..1728668) NCBI
• BioCyc: SA_RS08530 BioCyc
• MicrobesOnline: see SA1515

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAGTTTCACCACCGCTTAATGTTATTGATAAGCAGTATATTAATTATCAGTTTTGTT
  ACATTAGGCATTATTATTCACGCTTCCATCACATATGTTTTATCAGAAAAACACGAACAA
  GCTCTAGCAACAGATGCAAGATCATATATTTATTTAGTCCAAGATAATGAGATTGATAAA
  GTTAAAGAAATTGTAAAACAGCAAAATATAGATTTATTGATAACTAAACATGATAAAAAG
  GTGTTTAGTAGTGGCAAACTGAAAGCTATCCCAAATAGTGATAGCTATATTCAAAAAAGG
  GACGTTTTTATTTTTAATAAGAAGGTTAACGGTTATCATGTATGGATTAAAGGTTACAAT
  AACGATATTACAGAAATGCATTGGACGTTGTGGAAATATCTTATATTGACATGTTTAGTT
  GTACTCATTTGTCTGTATTTTGCAAGCCGTAGTTTTAAGCGTACATTAATCCGTCCCATT
  CAAGAAGTTACTTATGCAACACAATTGTTGGCAAATGGTTATTATCACATACGTGTACCT
  GAAAGTAATGTTGTAGAAACGAAAGCGCTTTTTGTGTCTACCAATGATTTAGCACGACGC
  CTTCAAAAGCTTAATAATGAGCAAAAAATTCAATCCAACCGTTTAAAAACAACGATTGAA
  AATATTCCAAGTGCTATTTTAATGATAGATCGAAATGGAAAAATCGTTGTTGCAAATAAA
  GCGTATTATGAACAGTTCAACATCTCGCATAATATTGAACAAGTAGGCTATCACGGCTAT
  GTTAATACTGAAATTGAGCAATTAATTTTAGAAAGTTTTAAAGTTGAAAAACCTATTTAT
  GAACAATTGGAAGTTGCAATAAACCAAGTCCATGCCAAGTACTTTGATATTTCATGCGTT
  CCGATTTTAACTAGGTCTCAAAAAAGTTTGCAAGGGATTTTAGTTGTGATGCATGATATA
  ACAAATCTGAAACAACTTGAAAATCTAAGACGTGAATTTGTAGCAAATGTTTCACATGAA
  TTAAAAACACCTATTACTTCAATTAAGGGGTTTGCTGAAACGCTTATTGATGGTGCAAAA
  AATGATGCGGAATCATTAGACATGTTTTTAAACATAATTTTAAAAGAATCAAATCGAATA
  GAATCTTTAGTAACAGATCTGTTAGATTTATCGCATATTGAGCAACATACAGAGTTAGAT
  ACAGACTATATGAATTTATCAGATTTAACGCGGCGTATTATTGATAATATGATGACTCAA
  GCCAATCAAAAAAATATTTCCATTCATACTGATATTGAAAAAGATGTCATTGTTAAAGCA
  CAAGAAAGTAAAATTGCTCAAGTTATCACGAATTTGTTAACGAATGCAATTAACTATTCT
  TATGAAGATGGAGATATTAACGTTCGTGTGTATCGAGATGACTTTCGTGTCATTTTCGAA
  GTACAAGATTTTGGTATAGGTATTAAATTAGAAGACCAACAACGTATTTTTGAAAGATTT
  TATAGAGTAGATAAAGCACGAAGTAGAGATTCTGGTGGGACAGGTCTTGGATTATCAATT
  ACAAAGCATATAGTAGAGGCCCACCAAGGCAATATTGAAGTGAATAGTCAAGTTGGCAAA
  GGCTCAACGTTCAAAGTTATTCTAAAAGATTATAAAGAATAA
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⊟Protein

⊟General

• locus tag: SA_RS08530 [old locus tag: SA1515 ]
• symbol: SA_RS08530
• description: Alkaline phosphatase synthesis sensor protein
• length: 553
• theoretical pI: 7.5548
• theoretical MW: 63773.9
• GRAVY: -0.201447

⊟Function

• reaction:
  EC 2.7.13.3  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine?
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 307.5)
  and 10 more
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 149.9)
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 142.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 70.8)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
  Signal transduction Regulatory functions Small molecule interactions PAS domain S-box protein (TIGR00229; HMM-score: 27.9)
  Metabolism Energy metabolism Photosynthesis transcriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
• TheSEED: see SA1515
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 91)
  and 10 more
  HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 70.5)
  PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 27.2)
  PAS_4; PAS fold (PF08448; HMM-score: 25)
  PAS_7; PAS fold (PF12860; HMM-score: 22.5)
  His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 19.9)
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 18.4)
  PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 18.2)
  PAS_8; PAS domain (PF13188; HMM-score: 17.7)
  no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 14)
  MKT1_N; Temperature dependent protein affecting M2 dsRNA replication (PF12247; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 28 aa
  • SP(Sec/SPI): 0.594169
  • TAT(Tat/SPI): 0.004119
  • LIPO(Sec/SPII): 0.009753
  • Cleavage Site: CS pos: 28-29. IHA-SI. Pr: 0.3558
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: see SA1515
• RefSeq: WP_015445861 NCBI
• UniProt: see SA1515

⊟Protein sequence

• MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALATDARSYIYLVQDNEIDKVKEIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDVFIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPIQEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTTIENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVEKPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFVANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSHIEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVITNLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARSRDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications