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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1739 [new locus tag: SACOL_RS08890 ]
  • pan locus tag?: SAUPAN004316000
  • symbol: SACOL1739
  • pan gene symbol?: phoR
  • synonym: pnpS
  • product: sensory box histidine kinase PhoR

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1739 [new locus tag: SACOL_RS08890 ]
  • symbol: SACOL1739
  • product: sensory box histidine kinase PhoR
  • replicon: chromosome
  • strand: -
  • coordinates: 1772699..1774363
  • length: 1665
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGAAGTTTCACCACCGCTTAATGTTATTGATAAGCAGTATATTAATTATCAGTTTT
    GTTACATTAGGCATTATTATTCACGCTTCCATCACATATGTTTTATCAGAAAAACACGAA
    CAAGCTCTAGCAACAGATGCAAGATCATATATTTATTTAGTCCAAGATAATGAGATTGAT
    AAAGTTAAAAAAATTGTAAAACAGCAAAATATAGATTTATTGATAACTAAGCATGATAAA
    AAGGTGTTTAGTAGTGGCAAACTGAAAGCTATCCCAAATAGTGATAGCTATATTCAAAAA
    AGGGACGTTTTTATTTTTAATAAGAAGGTTAACGGTTATCATGTATGGATTAAAGGTTAC
    AATAACGATATTACAGAAATGCATTGGACGTTGTGGAAATATCTTATTTTGACATGTTTA
    GTTGTACTCATTTGTCTGTATTTTGCAAGCCGTAGTTTTAAGCGTACATTAATCCGTCCC
    ATTCAAGAAGTTACTTATGCAACACAATTGTTGGCAAATGGTTATTATCACATACGTGTA
    CCTGAAAGTAATGTTGTAGAAACTAAAGCGCTTTTTGTGTCTACCAATGATTTAGCACGA
    CGCCTTCAAAAGCTTAATAATGAGCAAAAAATTCAATCCAACCGTTTAAAAACAACGATT
    GAAAATATTCCAAGTGCTATTTTAATGATAGATCGAAATGGAAAAATCGTTGTTGCAAAT
    AAAGCGTATTATGAACAGTTCAACATCTCGCATAATATTGAACAAGTAGGCTATCACGGC
    TATGTTAATACTGAAATTGAGCAATTAATTTTAGAAAGTTTTAAAGTTGAAAAACCTATT
    TATGAACAATTGGAAGTTGCAATAAACCAAGTCCATGCCAAGTACTTTGATATTTCATGC
    GTTCCGATTTTAACTAGGTCTCAAAAAAGTTTGCAAGGGATTTTAGTTGTGATGCATGAT
    ATAACAAATCTGAAACAACTTGAAAATCTAAGACGTGAATTTGTAGCAAATGTTTCACAT
    GAATTAAAAACACCTATTACTTCAATTAAGGGGTTTGCTGAAACGCTTATTGATGGTGCA
    AAAAATGATGCGGAATCATTAGACATGTTTTTAAACATAATTTTAAAAGAATCAAATCGA
    ATAGAATCTTTAGTAACAGATCTGTTAGATTTATCGCATATTGAGCAACATACAGAGTTA
    GATACAGACTATATGAATTTATCAGATTTAACGCGGCGTATTATTGATAATATGATGACT
    CAAGCCAATCAAAAAAATATTTCCATTCATACTGATATTGAAAAAGATGTCATTGTTAAA
    GCACAAGAAAGTAAAATTGCTCAAGTTATCACGAATTTGTTAACGAATGCAATTAACTAT
    TCTTATGAAGATGGAGATATTAACGTTCGTGTGTATCGAGATGACTTTCGTGTCATTTTC
    GAAGTACAAGATTTTGGTATAGGTATTAAATTAGAAGACCAACAACGTATTTTTGAAAGA
    TTTTATAGAGTAGATAAAGCACGAAGTAGAGATTCTGGTGGGACAGGTCTTGGATTATCA
    ATTACAAAGCATATAGTAGAGGCCCACCAAGGCAATATTGAAGTGAATAGTCAAGTTGGC
    AAAGGCTCAACGTTCAAAGTTATTCTAAAAGATTATAAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1665


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1739 [new locus tag: SACOL_RS08890 ]
  • symbol: SACOL1739
  • description: sensory box histidine kinase PhoR
  • length: 554
  • theoretical pI: 8.11061
  • theoretical MW: 63904.1
  • GRAVY: -0.198375

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.13.3  ExPASy
    Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine?
  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 307.5)
    and 10 more
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 149.9)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 142.3)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 70.8)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
    Signal transduction Regulatory functions Small molecule interactions PAS domain S-box protein (TIGR00229; HMM-score: 27.8)
    Metabolism Energy metabolism Photosynthesis transcriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
  • TheSEED  :
    • Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3)
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory High affinity phosphate transporter and control of PHO regulon  Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3)
    and 1 more
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3)
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 102.1)
    and 11 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 72.8)
    PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 27.9)
    PAS_4; PAS fold (PF08448; HMM-score: 25.1)
    PAS_8; PAS domain (PF13188; HMM-score: 25.1)
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 21.9)
    PAS_Fold (CL0183) PAS_7; PAS fold (PF12860; HMM-score: 21.1)
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 21)
    PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 15.5)
    SHS2 (CL0319) DUF6926; Domain of unknown function (DUF6926) (PF21977; HMM-score: 13.4)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 13.2)
    no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.9981
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0017
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 29 aa
    • SP(Sec/SPI): 0.565155
    • TAT(Tat/SPI): 0.004098
    • LIPO(Sec/SPII): 0.00787
    • Cleavage Site: CS pos: 29-30. IHA-SI. Pr: 0.3448
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MMKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALATDARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDVFIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPIQEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTTIENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVEKPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFVANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSHIEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVITNLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARSRDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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