PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS08890 [old locus tag: SACOL1739 ]
• pan locus tag^?: SAUPAN004316000
• symbol: SACOL_RS08890
• pan gene symbol^?: phoR
• synonym:
• product: sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08890 [old locus tag: SACOL1739 ]
• symbol: SACOL_RS08890
• product: sensor histidine kinase
• replicon: chromosome
• strand: -
• coordinates: 1772699..1774360
• length: 1662
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1772699..1774360) NCBI
• BioCyc: SACOL_RS08890 BioCyc
• MicrobesOnline: see SACOL1739

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAGTTTCACCACCGCTTAATGTTATTGATAAGCAGTATATTAATTATCAGTTTTGTT
  ACATTAGGCATTATTATTCACGCTTCCATCACATATGTTTTATCAGAAAAACACGAACAA
  GCTCTAGCAACAGATGCAAGATCATATATTTATTTAGTCCAAGATAATGAGATTGATAAA
  GTTAAAAAAATTGTAAAACAGCAAAATATAGATTTATTGATAACTAAGCATGATAAAAAG
  GTGTTTAGTAGTGGCAAACTGAAAGCTATCCCAAATAGTGATAGCTATATTCAAAAAAGG
  GACGTTTTTATTTTTAATAAGAAGGTTAACGGTTATCATGTATGGATTAAAGGTTACAAT
  AACGATATTACAGAAATGCATTGGACGTTGTGGAAATATCTTATTTTGACATGTTTAGTT
  GTACTCATTTGTCTGTATTTTGCAAGCCGTAGTTTTAAGCGTACATTAATCCGTCCCATT
  CAAGAAGTTACTTATGCAACACAATTGTTGGCAAATGGTTATTATCACATACGTGTACCT
  GAAAGTAATGTTGTAGAAACTAAAGCGCTTTTTGTGTCTACCAATGATTTAGCACGACGC
  CTTCAAAAGCTTAATAATGAGCAAAAAATTCAATCCAACCGTTTAAAAACAACGATTGAA
  AATATTCCAAGTGCTATTTTAATGATAGATCGAAATGGAAAAATCGTTGTTGCAAATAAA
  GCGTATTATGAACAGTTCAACATCTCGCATAATATTGAACAAGTAGGCTATCACGGCTAT
  GTTAATACTGAAATTGAGCAATTAATTTTAGAAAGTTTTAAAGTTGAAAAACCTATTTAT
  GAACAATTGGAAGTTGCAATAAACCAAGTCCATGCCAAGTACTTTGATATTTCATGCGTT
  CCGATTTTAACTAGGTCTCAAAAAAGTTTGCAAGGGATTTTAGTTGTGATGCATGATATA
  ACAAATCTGAAACAACTTGAAAATCTAAGACGTGAATTTGTAGCAAATGTTTCACATGAA
  TTAAAAACACCTATTACTTCAATTAAGGGGTTTGCTGAAACGCTTATTGATGGTGCAAAA
  AATGATGCGGAATCATTAGACATGTTTTTAAACATAATTTTAAAAGAATCAAATCGAATA
  GAATCTTTAGTAACAGATCTGTTAGATTTATCGCATATTGAGCAACATACAGAGTTAGAT
  ACAGACTATATGAATTTATCAGATTTAACGCGGCGTATTATTGATAATATGATGACTCAA
  GCCAATCAAAAAAATATTTCCATTCATACTGATATTGAAAAAGATGTCATTGTTAAAGCA
  CAAGAAAGTAAAATTGCTCAAGTTATCACGAATTTGTTAACGAATGCAATTAACTATTCT
  TATGAAGATGGAGATATTAACGTTCGTGTGTATCGAGATGACTTTCGTGTCATTTTCGAA
  GTACAAGATTTTGGTATAGGTATTAAATTAGAAGACCAACAACGTATTTTTGAAAGATTT
  TATAGAGTAGATAAAGCACGAAGTAGAGATTCTGGTGGGACAGGTCTTGGATTATCAATT
  ACAAAGCATATAGTAGAGGCCCACCAAGGCAATATTGAAGTGAATAGTCAAGTTGGCAAA
  GGCTCAACGTTCAAAGTTATTCTAAAAGATTATAAAGAATAA
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⊟Protein

⊟General

• locus tag: SACOL_RS08890 [old locus tag: SACOL1739 ]
• symbol: SACOL_RS08890
• description: sensor histidine kinase
• length: 553
• theoretical pI: 8.11061
• theoretical MW: 63772.9
• GRAVY: -0.20217

⊟Function

• reaction:
  EC 2.7.13.3  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine?
• TIGRFAM:
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 307.5)
  and 10 more
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 149.9)
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 141.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 77.2)
  proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 70.8)
  Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
  Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 46.6)
  Signal transduction Regulatory functions Small molecule interactions PAS domain S-box protein (TIGR00229; HMM-score: 27.8)
  Metabolism Energy metabolism Photosynthesis transcriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
  Signal transduction Regulatory functions DNA interactions transcriptional regulator PpsR (TIGR02040; HMM-score: 19.8)
• TheSEED: see SACOL1739
• PFAM:
  His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 91)
  and 10 more
  HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 70.5)
  PAS_Fold (CL0183) PAS; PAS fold (PF00989; HMM-score: 27.1)
  PAS_4; PAS fold (PF08448; HMM-score: 25)
  PAS_7; PAS fold (PF12860; HMM-score: 22.5)
  His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 20.4)
  PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 18.6)
  His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 18.4)
  PAS_Fold (CL0183) PAS_8; PAS domain (PF13188; HMM-score: 17.7)
  no clan defined SecD-TM1; SecD export protein N-terminal TM region (PF13721; HMM-score: 14.1)
  MKT1_N; Temperature dependent protein affecting M2 dsRNA replication (PF12247; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 2
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 28 aa
  • SP(Sec/SPI): 0.595768
  • TAT(Tat/SPI): 0.004057
  • LIPO(Sec/SPII): 0.009455
  • Cleavage Site: CS pos: 28-29. IHA-SI. Pr: 0.3568
• predicted transmembrane helices (TMHMM): 2

⊟Accession numbers

• GI: see SACOL1739
• RefSeq: WP_011443641 NCBI
• UniProt: see SACOL1739

⊟Protein sequence

• MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALATDARSYIYLVQDNEIDKVKKIVKQQNIDLLITKHDKKVFSSGKLKAIPNSDSYIQKRDVFIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPIQEVTYATQLLANGYYHIRVPESNVVETKALFVSTNDLARRLQKLNNEQKIQSNRLKTTIENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVEKPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFVANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSHIEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVITNLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARSRDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE

⊟Experimental data

• experimentally validated: see SACOL1739
• protein localization: see SACOL1739
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications