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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS08210 [old locus tag: SA1456 ]
  • pan locus tag?: SAUPAN004235000
  • symbol: SA_RS08210
  • pan gene symbol?: aspS
  • synonym:
  • product: aspartate--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS08210 [old locus tag: SA1456 ]
  • symbol: SA_RS08210
  • product: aspartate--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1660796..1662562
  • length: 1767
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1660796..1662562) NCBI
  • BioCyc: SA_RS08210 BioCyc
  • MicrobesOnline: see SA1456

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAGTAAGAGAACAACTTATTGTGGATTAGTTACTGAAGCATTTTTAGGACAAGAAATT
    ACATTAAAAGGATGGGTTAACAATCGTCGTGACCTAGGTGGATTGATTTTCGTTGATTTA
    AGAGATAGAGAAGGAATTGTACAAGTCGTGTTTAATCCTGCATTTTCAGAAGAGGCATTG
    AAAATTGCTGAAACAGTACGTTCTGAATATGTTGTAGAAGTTCAAGGTACAGTTACGAAG
    CGTGACCCTGAAACAGTTAATCCTAAAATTAAAACTGGCCAAGTTGAAGTACAAGTTACA
    AATATTAAAGTGATTAATAAATCTGAGACACCACCATTTTCTATAAATGAAGAAAATGTT
    AACGTTGATGAAAATATTCGATTAAAATACCGTTATTTAGATTTACGTCGTCAAGAGTTA
    GCGCAAACATTTAAAATGAGACATCAAATTACACGTTCTATTCGTCAATATTTGGATGAT
    GAAGGGTTCTTTGACATCGAAACACCAGTACTAACGAAGTCAACACCTGAGGGTGCACGT
    GACTATTTAGTACCATCTCGTGTTCATGATGGTGAATTTTATGCATTACCACAATCACCA
    CAATTATTTAAGCAATTATTGATGATTAGTGGATTTGACAAATACTACCAAATCGTAAAA
    TGCTTCCGTGACGAAGATTTACGTGCAGATCGTCAACCTGAATTCACACAAGTCGATATT
    GAAATGAGTTTTGTAGATCAAGAAGATGTGATGCAAATGGGTGAAGAAATGCTTAAAAAA
    GTTGTTAAAGAAGTTAAAGGCGTTGAAATTAATGGCGCTTTCCCACGCATGACATATAAA
    GAAGCGATGCGTCGCTATGGTTCTGATAAACCAGATACACGTTTTGAAATGGAATTAATT
    GACGTTTCTCAATTAGGACGTGATATGGACTTTAAAGTATTTAAAGATACTGTTGAAAAT
    GATGGTGAAATTAAAGCAATTGTCGCTAAAGGTGCAGCTGAACAATATACTCGTAAAGAT
    ATGGATGCTTTAACAGAATTTGTAAACATCTATGGTGCTAAAGGATTAGCGTGGGTTAAA
    GTTGTGGAAGATGGTTTGACAGGTCCAATTGGACGTTTCTTTGAAACAGAAAATGTTGAA
    ACATTACTTACATTAACTGGTGCTGAAGCTGGTGACTTAGTAATGTTTGTTGCAGACAAA
    CCAAATGTCGTTGCACAAAGTTTAGGTGCATTACGTGTCAAATTAGCTAAAGAATTAGGT
    TTAATCGATGAAACAAAATTAAACTTCTTATGGGTGACAGATTGGCCATTATTAGAATAT
    GATGAAGATGCGAAACGTTACGTTGCAGCACATCATCCATTTACATCTCCAAAAGAAGCT
    GATATTGCTAAGCTTGGCACTGCGCCAGAAGAAGCTGAGGCAAATGCTTATGACATAGTA
    TTAAATGGTTATGAATTAGGTGGCGGTTCAATCAGAATTCATGATGGTGAGTTACAAGAA
    AAAATGTTCGAAGTTCTTGGATTTACTAAAGAACAAGCACAAGAACAGTTCGGCTTCTTA
    CTAGATGCATTTAAATATGGTGCACCACCACATGGCGGTATTGCATTAGGATTAGACCGA
    TTAGTCATGTTATTGACTAATAGAACAAACTTGAGAGATACAATCGCATTCCCTAAAACA
    GCATCTGCTACATGTTTATTAACGAATGCGCCTGGTGAAGTTTCTGATAAACAATTAGAA
    GAACTTTCTTTGCGAATTCGTCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1767

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS08210 [old locus tag: SA1456 ]
  • symbol: SA_RS08210
  • description: aspartate--tRNA ligase
  • length: 588
  • theoretical pI: 4.69429
  • theoretical MW: 66598.4
  • GRAVY: -0.355272

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.12?  ExPASy
    Aspartate--tRNA ligase ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 855.8)
    and 7 more
    Genetic information processing Protein synthesis tRNA aminoacylation aspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 253.8)
    Genetic information processing Protein synthesis tRNA aminoacylation asparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 167.8)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 166.9)
    Unknown function General EF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 91.8)
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 24.4)
    Genetic information processing Protein synthesis tRNA aminoacylation pyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 16.2)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 9.9)
  • TheSEED: see SA1456
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 379.3)
    and 4 more
    GAD (CL0250) GAD; GAD domain (PF02938; HMM-score: 99.3)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 65.7)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 20.6)
    tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 16.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004914
    • TAT(Tat/SPI): 0.001285
    • LIPO(Sec/SPII): 0.00119
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDREGIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNIKVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDDEGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQIVKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPRMTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAEQYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGDLVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAAHHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGFTKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCLLTNAPGEVSDKQLEELSLRIRH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS02935DNA-directed RNA polymerase subunit beta'  [2] (data from MRSA252)
    SA_RS02955elongation factor G  [2] (data from MRSA252)
    SA_RS02960elongation factor Tu  [2] (data from MRSA252)
    SA_RS05355pyruvate dehydrogenase E1 component subunit beta  [2] (data from MRSA252)
    SA_RS05365dihydrolipoyl dehydrogenase  [2] (data from MRSA252)
    SA_RS06170succinyl-CoA ligase subunit alpha  [2] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [2] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [2] (data from MRSA252)
    SA_RS08560pyruvate kinase  [2] (data from MRSA252)
    SA_RS08625universal stress protein UspA  [2] (data from MRSA252)
    SA_RS11245glutamine--fructose-6-phosphate aminotransferase  [2] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [2] (data from MRSA252)
    SA_RS1160030S ribosomal protein S9  [2] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [2] (data from MRSA252)
    SA_RS1173530S ribosomal protein S3  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Asn) see SA1456

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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