PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS05815 [old locus tag: SA1020 ]
• pan locus tag^?: SAUPAN003446000
• symbol: SA_RS05815
• pan gene symbol^?: bshC
• synonym:
• product: bacillithiol biosynthesis cysteine-adding enzyme BshC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS05815 [old locus tag: SA1020 ]
• symbol: SA_RS05815
• product: bacillithiol biosynthesis cysteine-adding enzyme BshC
• replicon: chromosome
• strand: +
• coordinates: 1154519..1156132
• length: 1614
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1154519..1156132) NCBI
• BioCyc: SA_RS05815 BioCyc
• MicrobesOnline: see SA1020

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGACTGTAAAGTAGTTAGTTTAAATGAAAAAGATCAGTTTATACCAAAAATAAAGAGC
  AGTGACCCTGTAATAACAGGATTATTTCAATATGATGCAGCTCAACAAACTAGTTTTGAA
  AAAAGGATGTCTAAAGAAAATAATGGAAGAGAAGCGGCATTAGCGAATGTTATTCGTGAA
  TATATGAGTGATTTAAAGCTTTCAAATGAACAAGAATTAAACATACAACATTTAGCTAAT
  GGTTCAAAAGTTGTGATTGGTGGACAACAAGCAGGGCTTTTCGGGGGACCATTGTATACA
  TTCCATAAAATATTTTCAATCATTACTTTATCTAAGGAATTAACGGATACACATAAGCAA
  CAAGTAGTACCAGTTTTTTGGATTGCAGGAGAAGATCATGATTTCGATGAAGTGAATCAT
  ACATTTGTTTATAACGAAAATCATGGGTCGCTGCATAAGGTTAAATATCATACAATGGAG
  ATGCCAGAGACGACAGTCTCTAGATATTATCCTGATAAGGCTGAGTTGAAACAAACTTTA
  AAAACGATGTTCATTCATATGAAAGAAACTGTTCATACACAAGGTCTACTGGAGATTTGT
  GACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAA
  ACATTTAAAGCATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATG
  GAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATCAGTTGCTTGATGATGCTTTTAGA
  GCAACACAACAACGTACTCAAAATCAAGGCTTGAATGCGATGATACAAACAGATACAAAT
  GTTCATTTATTCTTACATGATGAAAATATGCGCCAATTAGTTTCGTATGATGGTAAGCAT
  TTTAAATTAAATAAAACAGATAAGACATATATAAAGGAAGAAATTATAAATATTGCGGAA
  AATCAACCTGAATTATTTTCTAATAATGTAGTGACAAGACCATTAATGGAAGAATGGTTA
  TTTAACACGGTGGCATTTGTTGGAGGACCGAGTGAAATTAAGTACTGGGCTGAACTAAAA
  GATGTATTTGAACTATTTGATGTTGAAATGCCTATCGTGATGCCAAGGCTTAGAATTACT
  TATTTAAATGACCGTATAGAAAAATTACTTTCGAAATACAATATTCCATTAGAAAAAGTG
  TTAGTCGATGGTGTTGAAGGAGAAAGAAGTAAGTTTATTAGAGAACAAGCATCACATCAA
  TTTATTGAAAAGGTAGAAGGTATGATTGAACAACAGCGTCGTCTAAACAAAGACTTATTA
  GATGAAGTGGCGGGGAATCAAAATAATATTAACCTTGTGAATAAAAATAATGAAATTCAT
  ATACAACAGTATGATTATTTGTTAAAACGTTATCTTTTAAACATTGAAAGAGAAAACGAC
  ATCAGTATGAAGCAATTTAGAGAAATTCAAGAAACACTCCATCCAATGGGAGGATTACAA
  GAAAGAATATGGAATCCACTTCAAATTTTGAATGATTTTGGGACAGATGTGTTCAAGCCC
  TCCACCTATCCACCACTTTCTTACACTTTTGATCATATTATTATAAAACCTTAA
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⊟Protein

⊟General

• locus tag: SA_RS05815 [old locus tag: SA1020 ]
• symbol: SA_RS05815
• description: bacillithiol biosynthesis cysteine-adding enzyme BshC
• length: 537
• theoretical pI: 6.12147
• theoretical MW: 62833.4
• GRAVY: -0.494227

⊟Function

• reaction:
  EC 6.-.-.-?  ExPASy
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs bacillithiol biosynthesis cysteine-adding enzyme BshC (TIGR03998; HMM-score: 627.7)
• TheSEED: see SA1020
• PFAM:
  HUP (CL0039) Rossmann-like_BshC; Bacillithiol biosynthesis BshC, N-terminal Rossmann-like domain (PF10079; HMM-score: 420.1)
  and 2 more
  no clan defined CC_BshC; Bacillithiol biosynthesis BshC, C-terminal coiled-coil domain (PF24850; HMM-score: 136.4)
  HHH (CL0198) Spo16_C; Spo16 protein, C-terminal (PF21698; HMM-score: 10.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9393
  • Cytoplasmic Membrane Score: 0.0554
  • Cell wall & surface Score: 0.0024
  • Extracellular Score: 0.0029
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004053
  • TAT(Tat/SPI): 0.00031
  • LIPO(Sec/SPII): 0.000603
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446262610 NCBI
• RefSeq: WP_000340465 NCBI
• UniProt: see SA1020

⊟Protein sequence

• MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMSKENNGREAALANVIREYMSDLKLSNEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFHKIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTMEMPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALLHETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQTDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRPLMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSKYNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNINLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQILNDFGTDVFKPSTYPPLSYTFDHIIIKP

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications