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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1190 [new locus tag: SACOL_RS06095 ]
  • pan locus tag?: SAUPAN003446000
  • symbol: SACOL1190
  • pan gene symbol?: bshC
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1190 [new locus tag: SACOL_RS06095 ]
  • symbol: SACOL1190
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1194105..1195718
  • length: 1614
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGACTGTAAAGTAGTTAGTTTAAATGAAAAAGATCAGTTTATACCAAAAATAAAGAGC
    AGTGACCCTGTAATAACAGGATTATTTCAATATGATGCAGCTCAACAAACTAGTTTTGAA
    AAAAGGATGTCTAAAGAAAATAATGGAAGAGAAGCGGCATTAGCGAATGTTATTCGTGAA
    TATATGAGTGATTTAAAGCTTTCAAGTGAACAAGAATTAAACATACAACATTTAGCTAAT
    GGTTCAAAAGTTGTGATTGGTGGACAACAAGCAGGGCTTTTCGGGGGACCATTGTATACA
    TTCCATAAAATATTTTCAATCATTACTTTATCTAAGGAATTAACGGATACACATAAGCAA
    CAAGTAGTACCAGTTTTTTGGATTGCAGGAGAAGATCATGATTTCGATGAAGTGAATCAT
    ACATTTGTTTATAACGAAAATCATGGGTCGCTGCATAAGGTTAAATATCATACAATGGAG
    ATGCCAGAGACGACTGTCTCTAGATATTATCCTGATAAGGCTGAGTTGAAACAAACTTTA
    AAAACGATGTTCATTCATATGAAAGAAACTGTTCATACACAAGGTCTACTGGAGATTTGT
    GACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAA
    ACATTTAAAGCATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATG
    GAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATCAGTTGCTTGATGATGCTTTTAGA
    GCAACACAACAACGTACTCAAAATCAAGGCTTGAATGCGATGATACAAACAGATACAAAT
    GTTCATTTATTCTTACATGATGAAAATATGCGTCAATTAGTTTCGTATGATGGTAAGCAT
    TTTAAATTAAATAAAACAGATAAGACATATATAAAGGAAGAAATTATAAATATTGCGGAA
    AATCAACCTGAATTATTTTCTAATAATGTAGTGACAAGACCATTAATGGAAGAATGGTTA
    TTTAACACGGTGGCATTTGTTGGAGGACCGAGTGAAATTAAGTACTGGGCTGAACTAAAA
    GATGTATTTGAACTATTTGATGTTGAAATGCCTATCGTGATGCCAAGGCTTAGAATTACT
    TATTTAAATGACCGTATAGAAAAATTACTTTCGAAATACAATATTCCATTAGAAAAAGTG
    TTAGTCGATGGTGTTGAAGGAGAAAGAAGTAAGTTTATTAGAGAACAAGCATCACATCAA
    TTTATTGAAAAGGTAGAAGGTATGATTGAACAACAGCGTCGTCTAAACAAAGACTTATTA
    GATGAAGTGGCGGGGAATCAAAATAATATTAACCTTGTGAATAAAAATAATGAAATTCAT
    ATACAACAGTATGATTATTTGTTAAAACGTTATCTTTTAAACATTGAAAGAGAAAACGAC
    ATCAGTATGAAGCAATTTAGAGAAATTCAAGAAACACTCCATCCAATGGGAGGATTACAA
    GAAAGAATATGGAATCTACTTCAAATTTTGAATGATTTTGGGACAGATGTGTTCAAGCCC
    TCCACCTATCCACCACTTTCTTACACTTTTGATCATATTATTATAAAACCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1614

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1190 [new locus tag: SACOL_RS06095 ]
  • symbol: SACOL1190
  • description: hypothetical protein
  • length: 537
  • theoretical pI: 6.12147
  • theoretical MW: 62822.4
  • GRAVY: -0.479143

⊟Function[edit | edit source]

  • reaction:
    EC 6.-.-.-?  ExPASy
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs bacillithiol biosynthesis cysteine-adding enzyme BshC (TIGR03998; HMM-score: 627.6)
  • TheSEED  :
    • BH2577 unknown conserved protein in B. subtilis
  • PFAM:
    HUP (CL0039) Rossmann-like_BshC; Bacillithiol biosynthesis BshC, N-terminal Rossmann-like domain (PF10079; HMM-score: 420.3)
    and 2 more
    no clan defined CC_BshC; Bacillithiol biosynthesis BshC, C-terminal coiled-coil domain (PF24850; HMM-score: 135)
    HHH (CL0198) Spo16_C; Spo16 protein, C-terminal (PF21698; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9319
    • Cytoplasmic Membrane Score: 0.0623
    • Cell wall & surface Score: 0.0025
    • Extracellular Score: 0.0032
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004655
    • TAT(Tat/SPI): 0.000332
    • LIPO(Sec/SPII): 0.000652
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMSKENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFHKIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTMEMPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALLHETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQTDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRPLMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSKYNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNINLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNLLQILNDFGTDVFKPSTYPPLSYTFDHIIIKP

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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