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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS06095 [old locus tag: SACOL1190 ]
- pan locus tag?: SAUPAN003446000
- symbol: SACOL_RS06095
- pan gene symbol?: bshC
- synonym:
- product: bacillithiol biosynthesis cysteine-adding enzyme BshC
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS06095 [old locus tag: SACOL1190 ]
- symbol: SACOL_RS06095
- product: bacillithiol biosynthesis cysteine-adding enzyme BshC
- replicon: chromosome
- strand: +
- coordinates: 1194105..1195718
- length: 1614
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1561ATGGACTGTAAAGTAGTTAGTTTAAATGAAAAAGATCAGTTTATACCAAAAATAAAGAGC
AGTGACCCTGTAATAACAGGATTATTTCAATATGATGCAGCTCAACAAACTAGTTTTGAA
AAAAGGATGTCTAAAGAAAATAATGGAAGAGAAGCGGCATTAGCGAATGTTATTCGTGAA
TATATGAGTGATTTAAAGCTTTCAAGTGAACAAGAATTAAACATACAACATTTAGCTAAT
GGTTCAAAAGTTGTGATTGGTGGACAACAAGCAGGGCTTTTCGGGGGACCATTGTATACA
TTCCATAAAATATTTTCAATCATTACTTTATCTAAGGAATTAACGGATACACATAAGCAA
CAAGTAGTACCAGTTTTTTGGATTGCAGGAGAAGATCATGATTTCGATGAAGTGAATCAT
ACATTTGTTTATAACGAAAATCATGGGTCGCTGCATAAGGTTAAATATCATACAATGGAG
ATGCCAGAGACGACTGTCTCTAGATATTATCCTGATAAGGCTGAGTTGAAACAAACTTTA
AAAACGATGTTCATTCATATGAAAGAAACTGTTCATACACAAGGTCTACTGGAGATTTGT
GACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAA
ACATTTAAAGCATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATG
GAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATCAGTTGCTTGATGATGCTTTTAGA
GCAACACAACAACGTACTCAAAATCAAGGCTTGAATGCGATGATACAAACAGATACAAAT
GTTCATTTATTCTTACATGATGAAAATATGCGTCAATTAGTTTCGTATGATGGTAAGCAT
TTTAAATTAAATAAAACAGATAAGACATATATAAAGGAAGAAATTATAAATATTGCGGAA
AATCAACCTGAATTATTTTCTAATAATGTAGTGACAAGACCATTAATGGAAGAATGGTTA
TTTAACACGGTGGCATTTGTTGGAGGACCGAGTGAAATTAAGTACTGGGCTGAACTAAAA
GATGTATTTGAACTATTTGATGTTGAAATGCCTATCGTGATGCCAAGGCTTAGAATTACT
TATTTAAATGACCGTATAGAAAAATTACTTTCGAAATACAATATTCCATTAGAAAAAGTG
TTAGTCGATGGTGTTGAAGGAGAAAGAAGTAAGTTTATTAGAGAACAAGCATCACATCAA
TTTATTGAAAAGGTAGAAGGTATGATTGAACAACAGCGTCGTCTAAACAAAGACTTATTA
GATGAAGTGGCGGGGAATCAAAATAATATTAACCTTGTGAATAAAAATAATGAAATTCAT
ATACAACAGTATGATTATTTGTTAAAACGTTATCTTTTAAACATTGAAAGAGAAAACGAC
ATCAGTATGAAGCAATTTAGAGAAATTCAAGAAACACTCCATCCAATGGGAGGATTACAA
GAAAGAATATGGAATCTACTTCAAATTTTGAATGATTTTGGGACAGATGTGTTCAAGCCC
TCCACCTATCCACCACTTTCTTACACTTTTGATCATATTATTATAAAACCTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS06095 [old locus tag: SACOL1190 ]
- symbol: SACOL_RS06095
- description: bacillithiol biosynthesis cysteine-adding enzyme BshC
- length: 537
- theoretical pI: 6.12147
- theoretical MW: 62822.4
- GRAVY: -0.479143
⊟Function[edit | edit source]
- reaction: EC 6.-.-.-? ExPASy
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs bacillithiol biosynthesis cysteine-adding enzyme BshC (TIGR03998; HMM-score: 627.6)
- TheSEED: see SACOL1190
- PFAM: HUP (CL0039) BshC; Bacillithiol biosynthesis BshC (PF10079; HMM-score: 523.7)and 1 moreno clan defined Minor_capsid_1; Minor capsid protein (PF10665; HMM-score: 13.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004655
- TAT(Tat/SPI): 0.000332
- LIPO(Sec/SPII): 0.000652
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMSKENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFHKIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTMEMPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALLHETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQTDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRPLMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSKYNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNINLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNLLQILNDFGTDVFKPSTYPPLSYTFDHIIIKP
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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