PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS05135 [old locus tag: SA0905 ]
• pan locus tag^?: SAUPAN003260000
• symbol: SA_RS05135
• pan gene symbol^?: atl
• synonym: atlA
• product: bifunctional autolysin

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS05135 [old locus tag: SA0905 ]
• symbol: SA_RS05135
• product: bifunctional autolysin
• replicon: chromosome
• strand: -
• coordinates: 1026342..1030088
• length: 3747
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1026342..1030088) NCBI
• BioCyc: SA_RS05135 BioCyc
• MicrobesOnline: see SA0905

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGCGAAAAAATTCAATTACAAACTACCATCAATGGTTGCATTAACGCTTGTAGGTTCA
  GCAGTCACTGCACATCAAGTTCAAGCAGCTGAGACGACACAAGATCAAACTACTAATAAA
  AATGTTTTAGATAGTAATAAAGTTAAAGCAACTACTGAACAAGCAAAAGCTGAGGTAAAA
  AATCCAACGCAAAACATTTCTGGCACTCAAGTATATCAAGACCCTGCTATTGTCCAACCA
  AAAACAGCAAATAACAAAACAGGCAATGCTCAAGTAAGTCAAAAAGTTGATACTGCACAA
  GTAAATGGTGACACTCGTGCTAATCAATCAGCGACTACAAATAATACGCAGCCTGTTGCA
  AAGTCAACAAGCACTACAGCACCTAAAACTAACACTAATGTTACAAATGCTGGTTATAGT
  TTAGTTGATGATGAAGATGATAATTCAGAACATCAAATTAATCCAGAATTAATTAAATCA
  GCTGCTAAACCTGCAGCTCTTGAAACGCAATATAAAGCCGCAGCACCTAAAGCTAAAACT
  GAAGCGACACCTAAAGTAACTACTTTTAGCGCTTCAGCACAACCAAGATCAGTTGCTGCA
  ACACCAAAAACGAGTTTGCCAAAATATAAACCACAAGTAAACTCTTCAATTAACGATTAC
  ATTCGTAAAAATAACTTAAAAGCACCTAAAATTGAAGAAGATTATACATCTTACTTCCCT
  AAATACGCATACCGTAACGGCGTAGGTCGTCCTGAAGGTATCGTAGTTCATGATACAGCT
  AATGATCGTTCGACGATAAATGGTGAAATTAGTTATATGAAAAATAACTATCAAAACGCA
  TTCGTACATGCATTTGTTGATGGGGATCGTATAATCGAAACAGCACCAACGGATTACTTA
  TCTTGGGGTGTCGGTGCAGTCGGTAACCCTAGATTCATCAATGTTGAAATCGTACACACA
  CACGACTATGCTTCATTTGCACGTTCAATGAATAACTATGCTGACTATGCAGCTACACAA
  TTACAATATTATGGTTTAAAACCAGACAGTGCTGAGTATGATGGAAATGGTACAGTATGG
  ACTCACTACGCTGTAAGTAAATATTTAGGTGGTACGGACCATGCCGATCCACATGGATAT
  TTAAGAAGTCATAATTATAGTTATGATCAATTATATGACTTAATTAATGAAAAATATTTA
  ATAAAAATGGGTAAAGTGGCGCCATGGGGTACGCAATTTACAACTACCCCTACTACACCA
  TCAAAACCAACAACACCGTCGAAACCATCAACTGGTAAATTAACAGTTGCAGCAAACAAT
  GGTGTCGCACAAATCAAACCAACAAATAGTGGTTTATATACTACTGTTTACGACAAAACT
  GGTAAAGCAACTAATGAAGTTCAAAAAACATTTGCTGTATCTAAAACAGCTACATTAGGT
  AATCAAAAATTCTATCTTGTTCAAGATTACAATTCTGGTAATAAATTTGGTTGGGTTAAA
  GAAGGCGATGTGGTTTACAACACAGCTAAATCACCTGTAAATGTAAATCAATCATATTCA
  ATCAAATCTGGTACGAAACTTTATACAGTACCTTGGGGTACATCTAAACAAGTTGCTGGT
  AGCGTGTCTGGCTCTGGAAACCAAACATTTAAGGCTTCAAAGCAACAACAAATTGATAAA
  TCAATTTATTTATATGGCTCTGTGAATGGTAAATCTGGTTGGGTAAGTAAAGCATATTTA
  GTTGATACTGCTAAACCTACGCCTACACCAATACCTAAGCCATCAACACCTACAACAAAT
  AATAAATTAACAGTTTCATCATTAAACGGTGTTGCTCAAATTAATGCTAAAAACAATGGC
  TTATTCACTACAGTTTATGACAAAACTGGTAAGCCAACGAAAGAAGTTCAAAAAACATTT
  GCTGTAACAAAAGAAGCAAGTTTAGGTGGAAACAAATTCTACTTAGTTAAAGATTACAAT
  AGTCCAACTTTAATTGGTTGGGTTAAACAAGGTGACGTTATTTATAACAATGCAAAATCA
  CCTGTAAATGTAATGCAAACATATACAGTAAAACCAGGCACTAAATTATATTCAGTACCT
  TGGGGCACTTATAAACAAGAAGCTGGTGCAGTTTCTGGTACAGGTAACCAAACTTTTAAA
  GCGACTAAGCAACAACAAATTGATAAATCTATCTATTTATTTGGAACTGTAAATGGTAAA
  TCTGGTTGGGTAAGTAAAGCATATTTAGCTGTACCTGCTGCACCTAAAAAAGCAGTAGCA
  CAACCAAAAACAGCTGTAAAAGCTTATACTGTTACTAAACCACAAACGACTCAAACAGTT
  AGCAAGATTGCTCAAGTTAAACCAAACAACACTGGTATTCGTGCTTCTGTTTATGAAAAA
  ACAGCGAAAAACGGTGCGAAATATGCAGACCGTACGTTCTATGTAACAAAAGAGCGTGCT
  CATGGTAATGAAACGTATGTATTATTAAACAATACAAGCCATAACATCCCATTAGGTTGG
  TTCAATGTAAAAGACTTAAATGTTCAAAACCTAGGCAAAGAAGTTAAAACGACTCAAAAA
  TATACTGTTAATAAATCAAATAACGGCTTATCAATGGTTCCTTGGGGTACTAAAAACCAA
  GTCATTTTAACAGGCAATAACATTGCTCAAGGTACATTTAATGCAACGAAACAAGTATCT
  GTAGGCAAAGATGTTTATTTATACGGTACTATTAATAACCGCACTGGTTGGGTAAATGCA
  AAAGATTTAACTGCACCAACTGCTGTGAAACCAACTACATCAGCTGCCAAAGATTATAAC
  TACACTTATGTAATTAAAAATGGTAATGGTTATTACTATGTAACACCAAATTCTGATACA
  GCTAAATACTCATTAAAAGCATTTAATGAACAACCATTCGCAGTTGTTAAAGAACAAGTC
  ATTAATGGACAAACTTGGTACTATGGTAAATTATCTAACGGTAAATTAGCATGGATTAAA
  TCAACTGATTTAGCTAAAGAATTAATTAAGTATAATCAAACAGGTATGACATTAAACCAA
  GTTGCTCAAATACAAGCTGGTTTACAATATAAACCACAAGTACAACGTGTACCAGGTAAG
  TGGACAGATGCTAACTTTAATGATGTTAAGCATGCAATGGATACGAAGCGTTTAGCTCAA
  GATCCAGCATTAAAATATCAATTCTTACGCTTAGACCAACCACAAAATATTTCTATTGAT
  AAAATTAATCAATTCTTAAAAGGTAAAGGTGTATTAGAAAACCAAGGTGCTGCATTTAAC
  AAAGCTGCTCAAATGTATGGCATTAATGAAGTTTATCTTATCTCACATGCCCTATTAGAA
  ACAGGTAACGGTACTTCTCAATTAGCGAAAGGTGCAGATGTAGTGAACAACAAAGTTGTA
  ACTAACTCAAACACGAAATACCATAACGTATTTGGTATTGCTGCATATGATAACGATCCT
  TTACGTGAAGGTATTAAATATGCTAAACAAGCTGGTTGGGACACAGTATCAAAAGCAATC
  GTTGGTGGTGCTAAATTCATCGGCAACTCATATGTAAAAGCTGGTCAAAATACACTTTAC
  AAAATGAGATGGAATCCTGCACATCCAGGAACACACCAATATGCTACAGATGTAGATTGG
  GCTAACATCAATGCTAAAATCATCAAAGGCTACTATGATAAAATTGGCGAAGTCGGCAAA
  TACTTCGACATCCCACAATATAAATAA
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⊟Protein

⊟General

• locus tag: SA_RS05135 [old locus tag: SA0905 ]
• symbol: SA_RS05135
• description: bifunctional autolysin
• length: 1248
• theoretical pI: 10.0803
• theoretical MW: 136749
• GRAVY: -0.612821

⊟Function

• reaction:
  EC 3.2.1.96?  ExPASy
  Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)₂)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact
  EC 3.5.1.28?  ExPASy
  N-acetylmuramoyl-L-alanine amidase Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, D subunit (TIGR00309; EC 3.6.3.14; HMM-score: 9.9)
• TheSEED: see SA0905
• PFAM:
  Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 54.2)
  SH3 (CL0010) SH3_8; SH3-like domain (PF13457; HMM-score: 46.4)
  and 3 more
  no clan defined DGOK; 2-keto-3-deoxy-galactonokinase (PF05035; HMM-score: 25.4)
  DUF780; Caenorhabditis elegans protein of unknown function (DUF780) (PF05611; HMM-score: 13.5)
  SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 9.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.02
  • Extracellular Score: 9.98
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 29 aa
  • SP(Sec/SPI): 0.997209
  • TAT(Tat/SPI): 0.001289
  • LIPO(Sec/SPII): 0.001006
  • Cleavage Site: CS pos: 29-30. VQA-AE. Pr: 0.9829
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446997278 NCBI
• RefSeq: WP_001074534 NCBI
• UniProt: see SA0905

⊟Protein sequence

• MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLDSNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKTANNKTGNAQVSQKVDTAQVNGDTRANQSATTNNTQPVAKSTSTTAPKTNTNVTNAGYSLVDDEDDNSEHQINPELIKSAAKPAALETQYKAAAPKAKTEATPKVTTFSASAQPRSVAATPKTSLPKYKPQVNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEISYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFARSMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNYSYDQLYDLINEKYLIKMGKVAPWGTQFTTTPTTPSKPTTPSKPSTGKLTVAANNGVAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWVKEGDVVYNTAKSPVNVNQSYSIKSGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQIDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPTPIPKPSTPTTNNKLTVSSLNGVAQINAKNNGLFTTVYDKTGKPTKEVQKTFAVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDVIYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSIYLFGTVNGKSGWVSKAYLAVPAAPKKAVAQPKTAVKAYTVTKPQTTQTVSKIAQVKPNNTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLNVQNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNATKQVSVGKDVYLYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKYSLKAFNEQPFAVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQVAQIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISIDKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNNKVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAGQNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKIGEVGKYFDIPQYK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, NCTC8325

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications