PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS05090 [old locus tag: SA0897 ]
• pan locus tag^?: SAUPAN003247000
• symbol: SA_RS05090
• pan gene symbol^?: menH
• synonym:
• product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS05090 [old locus tag: SA0897 ]
• symbol: SA_RS05090
• product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
• replicon: chromosome
• strand: +
• coordinates: 1018162..1018965
• length: 804
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1018162..1018965) NCBI
• BioCyc: SA_RS05090 BioCyc
• MicrobesOnline: see SA0897

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACACATTATAAATACTATGAAGCAAACGTTGAGACCAATCAAGTTTTAGTATTACTG
  CATGGTTTTCTTAGCGACAGTCGTACTTATTATAATCACATCGATAAATATACTGATATC
  TGTCATGTCATCACTATAGACTTACCAGGCCATGGCGAAGATCAGTCTTCAATGGATGAA
  ACGTGGAATTTTGATTATATTACGACGTTGTTAGACCGAATTTTAGATAAATATAAAGAT
  AAATCAATAACATTGTTTGGATATTCAATGGGTGGGCGTGTTGCATTATATTATGCAATT
  AATGGTCACATCCCTATATCTAATTTGATATTAGAAAGTACGTCACCAGGTATTAAAGAA
  GAAGCAAATCAATTGGAACGCCGTCTTGTTGATGATGCACGTGCTAAAGTATTAGACATA
  GCAGGTATTGAATTATTTGTTAATGATTGGGAAAAGTTGCCATTATTTCAATCGCAACTA
  GAATTACCAGTTGAAATACAACATCAAATAAGACTACAACGATTGTCTCAATCGCCACAG
  AAAATGGCCAAAGCATTAAGAGATTATGGTACAGGTCAAATGCCAAACTTATGGCCGCGC
  CTGAAAGAAATTAAAGTACCAACATTAATATTAGCTGGAGAATATGATGAAAAATTTGTA
  CAGATTGCGAAAAAAATGGCAAATTTAATTCCTAATAGTAAATGTAAATTAATTTCTGCT
  ACAGGTCATACAATTCATGTGGAAGATAGTGATGAATTTGATACAATGATATTAGGATTT
  TTAAAGGAGGAGCAAAATGACTAA
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⊟Protein

⊟General

• locus tag: SA_RS05090 [old locus tag: SA0897 ]
• symbol: SA_RS05090
• description: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
• length: 267
• theoretical pI: 5.19114
• theoretical MW: 30709.8
• GRAVY: -0.359176

⊟Function

• reaction:
  EC 4.2.99.20?  ExPASy
  2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase 5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 291.1)
  and 9 more
  proline-specific peptidase (TIGR01250; HMM-score: 78.5)
  Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 74.3)
  Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 57.5)
  pyrimidine utilization protein D (TIGR03611; HMM-score: 51.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 51.5)
  2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 38.9)
  Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 37)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 21.3)
  Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 19.5)
• TheSEED: see SA0897
• PFAM:
  AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 93.4)
  and 13 more
  Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 64.4)
  Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 50.7)
  Thioesterase; Thioesterase domain (PF00975; HMM-score: 25.9)
  Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 23.3)
  AB_hydrolase (CL0028) Lipase_3; Lipase (class 3) (PF01764; HMM-score: 19.5)
  DUF2974; Protein of unknown function (DUF2974) (PF11187; HMM-score: 19.2)
  Ndr; Ndr family (PF03096; HMM-score: 18.4)
  PGAP1; PGAP1-like protein (PF07819; HMM-score: 18.3)
  Esterase; Putative esterase (PF00756; HMM-score: 17.6)
  Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 16.3)
  DLH; Dienelactone hydrolase family (PF01738; HMM-score: 16.1)
  DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 14.1)
  BAAT_C; BAAT / Acyl-CoA thioester hydrolase C terminal (PF08840; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011885
  • TAT(Tat/SPI): 0.000268
  • LIPO(Sec/SPII): 0.003197
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446072342 NCBI
• RefSeq: WP_000150197 NCBI
• UniProt: see SA0897

⊟Protein sequence

• MTHYKYYEANVETNQVLVLLHGFLSDSRTYYNHIDKYTDICHVITIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAINGHIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQLELPVEIQHQIRLQRLSQSPQKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDEKFVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications