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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04430 [old locus tag: SA0776 ]
  • pan locus tag?: SAUPAN002897000
  • symbol: SA_RS04430
  • pan gene symbol?: sufS
  • synonym:
  • product: cysteine desulfurase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS04430 [old locus tag: SA0776 ]
  • symbol: SA_RS04430
  • product: cysteine desulfurase
  • replicon: chromosome
  • strand: +
  • coordinates: 882383..883633
  • length: 1251
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (882383..883633) NCBI
  • BioCyc: SA_RS04430 BioCyc
  • MicrobesOnline: see SA0776

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    GTGAATGAAGTGGCCGAACACTCATTTGACGTTAATGAAGTAATCAAGGATTTTCCGATA
    TTAGATCAAAAAGTCAATGGCAAACGTTTAGCATATCTTGATTCAACAGCGACAAGTCAA
    ACGCCTATGCAAGTGTTAAATGTTTTAGAGGATTACTACAAGCGTTATAATTCAAACGTT
    CATCGTGGTGTTCATACATTAGGATCATTGGCAACTGATGGTTATGAAAATGCCCGTGAA
    ACCGTTCGTCGTTTTATTAATGCGAAGTATTTTGAAGAAATCATTTTCACACGCGGAACA
    ACTGCGTCGATTAACCTTGTAGCACATAGCTATGGTGATGCAAATGTTGAAGAGGGCGAT
    GAAATTGTTGTCACTGAAATGGAGCATCATGCCAATATTGTTCCTTGGCAACAGTTAGCA
    AAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATC
    GAAGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATTTCT
    AATGTACTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGC
    GCAATTATCAGTGTTGATGGGGCGCAAGCAGCACCACATATGAAACTTGATATGCAAGAA
    ATGAATGCTGATTTTTATAGTTTTAGTGGTCATAAAATGCTTGGACCAACAGGTATTGGC
    GTATTATTTGGTAAACGTGAGTTACTACAAAAAATGGAACCGATTGAGTTCGGTGGTGAC
    ATGATTGATTTTGTAAGTAAGTATGATGCAACATGGGCTGATTTACCTACTAAATTTGAG
    GCGGGTACTCCATTAATTGCTCAAGCAATTGGGCTTGCAGAAGCTATTCGCTATTTAGAA
    CGCATAGGTTTTGATGCAATTCATAAATATGAACAAGAATTAACGATATATGCTTATGAG
    CAAATGTCTGCAATTGAAGGAATTGAAATTTATGGCCCGCCTAAGGATCGTCGTGCAGGT
    GTAATAACGTTTAATTTACAAGATGTACATCCACACGATGTTGCTACAGCCGTAGATACA
    GAAGGTGTAGCGGTTAGAGCTGGGCATCATTGTGCGCAACCGTTAATGAAATGGTTAAAT
    GTGTCTTCAACAGCTAGAGCGAGTTTTTATATATACAACACGAAAGAAGACATTGATCAG
    TTAATAAATGCCTTGAAACAAACGAAGGAGTTTTTCTCTTATGAATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS04430 [old locus tag: SA0776 ]
  • symbol: SA_RS04430
  • description: cysteine desulfurase
  • length: 416
  • theoretical pI: 5.18484
  • theoretical MW: 46664.5
  • GRAVY: -0.26274

⊟Function[edit | edit source]

  • reaction:
    EC 2.8.1.7?  ExPASy
    Cysteine desulfurase L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 615.7)
    and 33 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 384.5)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 260.9)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 251.8)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 167.9)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 145)
    Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 136.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 136.3)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 120.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 71.1)
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 43)
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 41.6)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 33.2)
    Metabolism Energy metabolism Amino acids and amines kynureninase (TIGR01814; EC 3.7.1.3; HMM-score: 33.2)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 33.1)
    Metabolism Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 31.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 31.2)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 27.6)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 27.5)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 27.5)
    Genetic information processing Protein synthesis tRNA aminoacylation L-seryl-tRNA(Sec) selenium transferase (TIGR00474; EC 2.9.1.1; HMM-score: 25.6)
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 25.2)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 23)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 22.5)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 21.1)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides TDP-4-keto-6-deoxy-D-glucose transaminase (TIGR02379; HMM-score: 21)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 18)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 16.4)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 16)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin 8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 15.2)
    Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 15.2)
    Unknown function Enzymes of unknown specificity pyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 15)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 14.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 14.1)
  • TheSEED: see SA0776
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 537)
    and 6 more
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 44.4)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 37.2)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 36.2)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 32.5)
    SelA; L-seryl-tRNA selenium transferase (PF03841; HMM-score: 21.3)
    Pyridoxal_deC; Pyridoxal-dependent decarboxylase conserved domain (PF00282; HMM-score: 17)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005983
    • TAT(Tat/SPI): 0.000713
    • LIPO(Sec/SPII): 0.001314
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPMQVLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTASINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNIEDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDMQEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLPTKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPPKDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIYNTKEDIDQLINALKQTKEFFSYEF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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