PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS02220 [old locus tag: SA0387 ]
• pan locus tag^?: SAUPAN002113000
• symbol: SA_RS02220
• pan gene symbol^?: ssl7
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS02220 [old locus tag: SA0387 ]
• symbol: SA_RS02220
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 446880..447575
• length: 696
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (446880..447575) NCBI
• BioCyc: SA_RS02220 BioCyc
• MicrobesOnline: see SA0387

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATTAAAAACGTTAGCTAAAGCAACATTGGCATTAGGCTTATTAACTACTGGTGTG
  ATTACATCAGAAGGCCAAGCAGTCCACGCAAAAGAAAAGCAAGAGAGAGTACAACATTTA
  TATGATATTAAAGACTTATATCGATACTACTCATCAGAAAGTTTTGAATTCAGTAATATT
  AGTGGTAAGGTTGAAAACTATAACGGTTCTAACGTTGTACGCTTTAACCAAGAAAAACAA
  AATCACCAATTATTCTTATTAGGAAAAGATAAAGATAAATATAAAAAAGGCCTTGAAGGC
  CAGAATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGACTATCTACTGTTGGT
  GGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAA
  GTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTTAATTAATAAAGAAGAAGTT
  TCACTGAAAGAACTTGATTTCAAAATTAGAAAGCAATTAGTTGAAAAATATGGTTTATAT
  AAAGGTACGACTAAATACGGTAAGATCACTATCAATTTGAAAGACGAGAAAAAGGAAGTA
  ATTGATTTAGGTGATAAACTGCAATTCGAGCGCATGGGTGATGTGTTGAATAGTAAGGAT
  ATTCAAAATATAGCAGTGACTATTAATCAAATTTAA
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⊟Protein

⊟General

• locus tag: SA_RS02220 [old locus tag: SA0387 ]
• symbol: SA_RS02220
• description: hypothetical protein
• length: 231
• theoretical pI: 9.79031
• theoretical MW: 26202.7
• GRAVY: -0.580519

⊟Function

• TIGRFAM:
• TheSEED: see SA0387
• PFAM:
  OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 101.2)
  Ubiquitin (CL0072) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 95.6)
  and 2 more
  no clan defined MAP; MAP domain (PF03642; HMM-score: 15.2)
  SH3 (CL0010) BAH; BAH domain (PF01426; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0018
  • Cell wall & surface Score: 0.0864
  • Extracellular Score: 0.9118
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.988663
  • TAT(Tat/SPI): 0.002021
  • LIPO(Sec/SPII): 0.005649
  • Cleavage Site: CS pos: 30-31. VHA-KE. Pr: 0.8600
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446692474 NCBI
• RefSeq: WP_000769820 NCBI
• UniProt: see SA0387

⊟Protein sequence

• MKLKTLAKATLALGLLTTGVITSEGQAVHAKEKQERVQHLYDIKDLYRYYSSESFEFSNISGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDKYKKGLEGQNVFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFLINKEEVSLKELDFKIRKQLVEKYGLYKGTTKYGKITINLKDEKKEVIDLGDKLQFERMGDVLNSKDIQNIAVTINQI

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SA0387

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)