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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS02220 [old locus tag: SA0387 ]
  • pan locus tag?: SAUPAN002113000
  • symbol: SA_RS02220
  • pan gene symbol?: ssl7
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS02220 [old locus tag: SA0387 ]
  • symbol: SA_RS02220
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 446880..447575
  • length: 696
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (446880..447575) NCBI
  • BioCyc: SA_RS02220 BioCyc
  • MicrobesOnline: see SA0387

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAATTAAAAACGTTAGCTAAAGCAACATTGGCATTAGGCTTATTAACTACTGGTGTG
    ATTACATCAGAAGGCCAAGCAGTCCACGCAAAAGAAAAGCAAGAGAGAGTACAACATTTA
    TATGATATTAAAGACTTATATCGATACTACTCATCAGAAAGTTTTGAATTCAGTAATATT
    AGTGGTAAGGTTGAAAACTATAACGGTTCTAACGTTGTACGCTTTAACCAAGAAAAACAA
    AATCACCAATTATTCTTATTAGGAAAAGATAAAGATAAATATAAAAAAGGCCTTGAAGGC
    CAGAATGTCTTTGTGGTAAAAGAATTAATTGATCCAAACGGTAGACTATCTACTGTTGGT
    GGTGTGACTAAGAAAAATAACAAATCTTCTGAAACTAATACACATTTATTTGTTAATAAA
    GTGTATGGCGGAAATTTAGATGCATCAATTGACTCATTTTTAATTAATAAAGAAGAAGTT
    TCACTGAAAGAACTTGATTTCAAAATTAGAAAGCAATTAGTTGAAAAATATGGTTTATAT
    AAAGGTACGACTAAATACGGTAAGATCACTATCAATTTGAAAGACGAGAAAAAGGAAGTA
    ATTGATTTAGGTGATAAACTGCAATTCGAGCGCATGGGTGATGTGTTGAATAGTAAGGAT
    ATTCAAAATATAGCAGTGACTATTAATCAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    696

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS02220 [old locus tag: SA0387 ]
  • symbol: SA_RS02220
  • description: hypothetical protein
  • length: 231
  • theoretical pI: 9.79031
  • theoretical MW: 26202.7
  • GRAVY: -0.580519

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA0387
  • PFAM:
    OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 101)
    and 2 more
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 37.4)
    MAP; MAP domain (PF03642; HMM-score: 16.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.988663
    • TAT(Tat/SPI): 0.002021
    • LIPO(Sec/SPII): 0.005649
    • Cleavage Site: CS pos: 30-31. VHA-KE. Pr: 0.8600
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKLKTLAKATLALGLLTTGVITSEGQAVHAKEKQERVQHLYDIKDLYRYYSSESFEFSNISGKVENYNGSNVVRFNQEKQNHQLFLLGKDKDKYKKGLEGQNVFVVKELIDPNGRLSTVGGVTKKNNKSSETNTHLFVNKVYGGNLDASIDSFLINKEEVSLKELDFKIRKQLVEKYGLYKGTTKYGKITINLKDEKKEVIDLGDKLQFERMGDVLNSKDIQNIAVTINQI

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol Med Microbiol: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)