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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS02040 [old locus tag: SA0357 ]
  • pan locus tag?: SAUPAN001967000
  • symbol: SA_RS02040
  • pan gene symbol?: selX
  • synonym:
  • product: toxin

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS02040 [old locus tag: SA0357 ]
  • symbol: SA_RS02040
  • product: toxin
  • replicon: chromosome
  • strand: +
  • coordinates: 415162..415773
  • length: 612
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (415162..415773) NCBI
  • BioCyc: SA_RS02040 BioCyc
  • MicrobesOnline: see SA0357

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTCAAAAAATATGACTCAAAAAATTCAATCGTATTAAAATCTATTCTATCGCTAGGT
    ATCATCTATGGGGGAACATTTGGAATATATCCAAAAGCAGACGCGTCAACACAAAATTCC
    TCAAGTGTACAAGATAAACAATTACAAAAAGTTGAAGAAGTACCAAATAATTCAGAAAAA
    GCTTTGGTTAAAAAACTTTACGATAGATACAGCAAGGATACAATAAATGGAAAATCTAAT
    AAATCTAGGAATTGGGTTTATTCAGAGAGACCTTTAAATGAAAACCAAGTTCGTATACAT
    TTAGAAGGAACATACACAGTTGCTGATAGAGTATATACACCTAAGAGAAATATTACTCTT
    AATAAAGAAGTTGTCACTTTAAAGGAATTGGATCATATCATAAGATTTGCTCATATTTCT
    TATGGCTTATATATGGGAGAACATTTGCCTAAAGGTAACATCGTCATAAATACAAAAGAT
    GGCGGTAAATATACATTAGAGTCGCATAAAGAGCTACAAAAAGATAGGGAAAATGTAAAA
    ATTAATACAGCCGATATAAAAAATGTAACTTTCAAACTTGTGAAAAGTGTTAATGACATT
    GAACAAGTTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    612

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS02040 [old locus tag: SA0357 ]
  • symbol: SA_RS02040
  • description: toxin
  • length: 203
  • theoretical pI: 9.97891
  • theoretical MW: 23223.3
  • GRAVY: -0.671429

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SA0357
  • PFAM:
    Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 21.9)
    and 2 more
    no clan defined EAR; Ethylene-responsive binding factor-associated repression (PF07897; HMM-score: 12.8)
    Ubiquitin (CL0072) CHIPS; Chemotaxis-inhibiting protein CHIPS (PF11434; HMM-score: 11.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 35 aa
    • SP(Sec/SPI): 0.819885
    • TAT(Tat/SPI): 0.029227
    • LIPO(Sec/SPII): 0.013965
    • Cleavage Site: CS pos: 35-36. ADA-ST. Pr: 0.7459
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MFKKYDSKNSIVLKSILSLGIIYGGTFGIYPKADASTQNSSSVQDKQLQKVEEVPNNSEKALVKKLYDRYSKDTINGKSNKSRNWVYSERPLNENQVRIHLEGTYTVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKDGGKYTLESHKELQKDRENVKINTADIKNVTFKLVKSVNDIEQV

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Gillian J Wilson, Keun Seok Seo, Robyn A Cartwright, Timothy Connelley, Olivia N Chuang-Smith, Joseph A Merriman, Caitriona M Guinane, Joo Youn Park, Gregory A Bohach, Patrick M Schlievert, W Ivan Morrison, J Ross Fitzgerald
A novel core genome-encoded superantigen contributes to lethality of community-associated MRSA necrotizing pneumonia.
PLoS Pathog: 2011, 7(10);e1002271
[PubMed:22022262] [WorldCat.org] [DOI] (I p)