PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS01330 [old locus tag: SA0227 ]
• pan locus tag^?: SAUPAN001096000
• symbol: SA_RS01330
• pan gene symbol^?: fadX
• synonym:
• product: acetyl-CoA/acetoacetyl-CoA transferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS01330 [old locus tag: SA0227 ]
• symbol: SA_RS01330
• product: acetyl-CoA/acetoacetyl-CoA transferase
• replicon: chromosome
• strand: -
• coordinates: 273754..275331
• length: 1578
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (273754..275331) NCBI
• BioCyc: SA_RS01330 BioCyc
• MicrobesOnline: see SA0227

⊟Phenotype

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⊟DNA sequence

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  ATGGAGGTGACTTGCTTGAAACAAATCACATGGCACGACTTACAACATATCATTAAAGAT
  GGTGATGTGATTGGTTTACCAGCATTAGCTGTAGCCAACTTACCCGCCGAAGTTCTACGT
  GCTGTGTTAGCGCAACATGACACATATCATACGCCCAAAGATTTAACGTTTATATTAGCG
  AATGATATCCATAGTTTAGGTGCCGCACCGGATTTAGATGATTTTATAGAACGTCGCATG
  ATTAAACGTGTCATTATGAGCATTTTAACGGCTTCTTCCAAAACGGCACAAGCAATGAAA
  AATAATGACATTGAAGCTTATTTTTTACCACAAGGTATCATTGCAACTCATTATCGTCAG
  AGTAATCAATTATTACCTGGAGTTATTACTAAAATCGGATTAAACACAGCTGTTGATCCT
  AGATACGGTGGCGGTAAAGTAAATACACGAACAACTGATGATTTAGTTTCATTAGTAACC
  ATCAACGATGAAACATACTTACATTACACATTCCCTAGCGTTGATGTGGCACTACTGAGA
  GGAACATACGCAGATCAACAAGGTAACATTTATTTAACTCAAGAAGCGTACTTGAGCGAG
  TGTTATCATGTCGCATTAAACGCGAAAGCCAATCATGGGAAAGTTATTGTACAAGTTAAA
  GCTTTAGTTGACGACTATCAACTAAAACCGAATGAAGTTGTTATCCCAGGAAATCTTGTC
  GATTATGTATACGTCACAGAAGATGAAAAGAATCACCGCCAAGTAATTCAGAGTCATTAT
  TTACCAGCCTTGTCTGGAGAAGAACGAATTGATGGAATACCTGAACCCGCATTACCTTTT
  AATAGTCGCAAATTGATTCTCCGACGTGCTGCTCAGTTTTTAACTTATGGCGATACAATT
  AGCATCGGTTATGGCATCAATAATGAACTCTCTAATTTATTGCACGAAGAATGTGTTGAA
  CATGATGTGCAACCGATTCTAGATGTTGGCATTTTCGGTGGATTCGTTGGGAGTCGTGAA
  CATTTTGGTATGAATTACAATGCAGATGTGCGCATGCCTCATGATCGAGCATGGGATTTT
  ATTTATAACAATGGTGTATCAGTTGCCTATCTTAGCTTTGCTGAGGTTGATCAATACGGC
  AATGTCAACGTGTCTTACTTCAATGACCGACTAAATGGATGTGGTGGCTTTATAGACATT
  ACGCAATCTGTAAATAAAATTATCTTTTCAGGTACTTTTGTAGCTGGCAGTCATGTCTCA
  TGTCATAATCAACGATTAAACATTGAAACTGAAGGACAAAACCAGAAATTTGTATCAGAT
  GTGAGCCATATCGACTTTAATGCACAATATTCACAATTACTCGAGCAAGAAGTCTATTTT
  GTTACTGATCGTGCAGTATTCGAGCTCACCAATCAAGGCTTGAAACTAATTGAAATTGCA
  CCAGGTCTTGATTTGCATAAAGATATTTTGAATCAAATGGCTTTTAAACCAATTATTGCT
  GATCATTTAAAATTAATTGATACCAGCATTTACAAAGAAAAATGGGGACAACTTAAACAA
  TCAATTCATAAAGTATGA
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⊟Protein

⊟General

• locus tag: SA_RS01330 [old locus tag: SA0227 ]
• symbol: SA_RS01330
• description: acetyl-CoA/acetoacetyl-CoA transferase
• length: 525
• theoretical pI: 5.62104
• theoretical MW: 58902.3
• GRAVY: -0.15581

⊟Function

• TIGRFAM:
  3-oxoacid CoA-transferase, A subunit (TIGR02429; EC 2.8.3.-; HMM-score: 41.5)
  and 3 more
  3-oxoacid CoA-transferase, B subunit (TIGR02428; EC 2.8.3.-; HMM-score: 26.8)
  Metabolism Transport and binding proteins Cations and iron carrying compounds malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.5)
  Metabolism Energy metabolism Other malonate decarboxylase, alpha subunit (TIGR01110; HMM-score: 23.5)
• TheSEED: see SA0227
• PFAM:
  ISOCOT_Fold (CL0246) CoA_trans; Coenzyme A transferase (PF01144; HMM-score: 71.7)
  and 3 more
  Mal_decarbox_Al; Malonate decarboxylase, alpha subunit, transporter (PF16957; HMM-score: 22.4)
  AcetylCoA_hydro; Acetyl-CoA hydrolase/transferase N-terminal domain (PF02550; HMM-score: 19.1)
  no clan defined Curlin_rpt; Curlin associated repeat (PF07012; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012359
  • TAT(Tat/SPI): 0.002349
  • LIPO(Sec/SPII): 0.000585
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SA0227
• RefSeq: WP_001795358 NCBI
• UniProt: see SA0227

⊟Protein sequence

• MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLAQHDTYHTPKDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNNDIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVTINDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNAKANHGKVIVQVKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEPALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGGFVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQYGNVNVSYFNDRLNGCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQYSQLLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDTSIYKEKWGQLKQSIHKV

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SA0227

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications