PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS01295 [old locus tag: SA0222 ]
• pan locus tag^?: SAUPAN001088000
• symbol: SA_RS01295
• pan gene symbol^?: coa
• synonym:
• product: coagulase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS01295 [old locus tag: SA0222 ]
• symbol: SA_RS01295
• product: coagulase
• replicon: chromosome
• strand: +
• coordinates: 264676..266652
• length: 1977
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (264676..266652) NCBI
• BioCyc: SA_RS01295 BioCyc
• MicrobesOnline: see SA0222

⊟Phenotype

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⊟DNA sequence

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  ATGAAAAAGCAAATAATTTCGCTAGGCGCATTAGCAGTTGCATCTAGCTTATTTACATGG
  GATAACAAAGCAGATGCGATAGTAACAAAGGATTATAGTAAAGAATCAAGAGTGAATGAG
  AAAAGTAAAAAGGGAGCTACTGTTTCAGATTATTACTATTGGAAAATAATTGATAGTTTA
  GAGGCACAATTTACTGGAGCAATAGACTTATTGGAAGATTATAAATATGGAGATCCTATC
  TATAAAGAAGCGAAAGATAGATTGATGACAAGAGTATTAGGAGAAGACCAGTATTTATTA
  AAGAAAAAGATTGATGAATATGAGCTTTATAAAAAGTGGTATAAAAGTTCAAATAAGAAC
  ACTAATATGCTTACTTTCCATAAATATAATCTTTACAATTTAACAATGAATGAATATAAC
  GATATTTTTAACTCTTTGAAAGATGCAGTTTATCAATTTAATAAAGAAGTTAAAGAAATA
  GAGCATAAAAATGTTGACTTGAAGCAGTTTGATAAAGATGGAGAAGACAAGGCAACTAAA
  GAAGTTTATGACCTTGTTTCTGAAATTGATACATTAGTTGTAACTTATTATGCTGATAAG
  GATTATGGGGAGCATGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACA
  GACAATCCACATAAAATTACAAATGAGCGTATAAAAAAAGAAATGATCGATGACTTAAAT
  TCAATTATAGATGATTTCTTTATGGAGACTAAACAAAATAGACCGAATTCTATAACAAAA
  TATGATCCAACAAAACACAATTTTAAAGAGAAGAGTGAAAATAAACCTAATTTTGATAAA
  TTAGTTGAAGAAACAAAAAAAGCAGTTAAAGAAGCAGACGAATCTTGGAAAAATAAAACT
  GTCAAAAAATACGAGGAAACTGTAACAAAATCTCCTGTTGTAAAAGAAGAGAAGAAAGTT
  GAAGAACCTCAATTACCTAAAGTTGGAAACCAGCAAGAGGTTAAAACTACGGCTGGTAAA
  GCTGAAGAAACAACACAACCAGTGGCACAGCCATTAGTAAAAATTCCACAAGAAACAATC
  TATGGTGAAACTGTAAAAGGTCCAGAATATCCAACGATGGAAAATAAAACGTTACAAGGT
  GAAATCGTTCAAGGTCCCGATTTTCTAACAATGGAACAAAACAGACCATCTTTAAGCGAT
  AATTATACTCAACCGACGACACCGAACCCTATTTTAGAAGGTCTTGAAGGTAGCTCATCT
  AAACTTGAAATAAAACCACAAGGTACTGAATCAACGTTGAAAGGTATTCAAGGAGAATCA
  AGTGATATTGAAGTTAAACCTCAAGCAACTGAAACAACAGAAGCTTCTCAATATGGTCCG
  AGACCGCAATTTAACAAAACACCTAAGTATGTGAAATATAGAGATGCTGGTACAGGTATC
  CGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAACAAGCCAAGT
  GAAACAAATGCATACAACGTAACGACAAATCAAGATGGCACAGTATCATACGGAGCTCGC
  CCAACACAAAACAAGCCAAGTGAAACAAACGCATATAACGTAACAACACATGCAAATGGT
  CAAGTATCATACGGTGCTCGCCCAACACAAAAAAAGCCAAGCAAAACAAATGCATACAAC
  GTAACAACACATGCAAATGGTCAAGTATCATATGGCGCTCGCCCGACACAAAAAAAGCCA
  AGCAAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCT
  CGCCCGACATACAAGAAGCCAAGCGAAACAAATGCATACAACGTAACAACACATGCAAAT
  GGTCAAGTATCATATGGCGCTCGCCCGACACAAAAAAAGCCAAGCGAAACAAACGCATAT
  AACGTAACAACACATGCAGATGGTACTGCGACATATGGGCCTAGAGTAACAAAATAA
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⊟Protein

⊟General

• locus tag: SA_RS01295 [old locus tag: SA0222 ]
• symbol: SA_RS01295
• description: coagulase
• length: 658
• theoretical pI: 8.74741
• theoretical MW: 74519.3
• GRAVY: -1.04301

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines mitochondrial import receptor subunit (TIGR00985; HMM-score: 13)
• TheSEED: see SA0222
• PFAM:
  no clan defined Coagulase; Staphylococcus aureus coagulase (PF08764; HMM-score: 452)
  and 2 more
  Staphylcoagulse; Staphylocoagulase repeat (PF04022; HMM-score: 333.6)
  Ntox25; Bacterial toxin 25 (PF15530; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.985782
  • TAT(Tat/SPI): 0.00152
  • LIPO(Sec/SPII): 0.001861
  • Cleavage Site: CS pos: 26-27. ADA-IV. Pr: 0.9147
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446666750 NCBI
• RefSeq: WP_000744096 NCBI
• UniProt: see SA0222

⊟Protein sequence

• MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNEKSKKGATVSDYYYWKIIDSLEAQFTGAIDLLEDYKYGDPIYKEAKDRLMTRVLGEDQYLLKKKIDEYELYKKWYKSSNKNTNMLTFHKYNLYNLTMNEYNDIFNSLKDAVYQFNKEVKEIEHKNVDLKQFDKDGEDKATKEVYDLVSEIDTLVVTYYADKDYGEHAKELRAKLDLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPNSITKYDPTKHNFKEKSENKPNFDKLVEETKKAVKEADESWKNKTVKKYEETVTKSPVVKEEKKVEEPQLPKVGNQQEVKTTAGKAEETTQPVAQPLVKIPQETIYGETVKGPEYPTMENKTLQGEIVQGPDFLTMEQNRPSLSDNYTQPTTPNPILEGLEGSSSKLEIKPQGTESTLKGIQGESSDIEVKPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTNQDGTVSYGARPTQNKPSETNAYNVTTHANGQVSYGARPTQKKPSKTNAYNVTTHANGQVSYGARPTQKKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHANGQVSYGARPTQKKPSETNAYNVTTHADGTATYGPRVTK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SA0222

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications