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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0222 [new locus tag: SA_RS01295 ]
  • pan locus tag?: SAUPAN001088000
  • symbol: coa
  • pan gene symbol?: coa
  • synonym:
  • product: staphylocoagulase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0222 [new locus tag: SA_RS01295 ]
  • symbol: coa
  • product: staphylocoagulase
  • replicon: chromosome
  • strand: +
  • coordinates: 264676..266652
  • length: 1977
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAAAAAGCAAATAATTTCGCTAGGCGCATTAGCAGTTGCATCTAGCTTATTTACATGG
    GATAACAAAGCAGATGCGATAGTAACAAAGGATTATAGTAAAGAATCAAGAGTGAATGAG
    AAAAGTAAAAAGGGAGCTACTGTTTCAGATTATTACTATTGGAAAATAATTGATAGTTTA
    GAGGCACAATTTACTGGAGCAATAGACTTATTGGAAGATTATAAATATGGAGATCCTATC
    TATAAAGAAGCGAAAGATAGATTGATGACAAGAGTATTAGGAGAAGACCAGTATTTATTA
    AAGAAAAAGATTGATGAATATGAGCTTTATAAAAAGTGGTATAAAAGTTCAAATAAGAAC
    ACTAATATGCTTACTTTCCATAAATATAATCTTTACAATTTAACAATGAATGAATATAAC
    GATATTTTTAACTCTTTGAAAGATGCAGTTTATCAATTTAATAAAGAAGTTAAAGAAATA
    GAGCATAAAAATGTTGACTTGAAGCAGTTTGATAAAGATGGAGAAGACAAGGCAACTAAA
    GAAGTTTATGACCTTGTTTCTGAAATTGATACATTAGTTGTAACTTATTATGCTGATAAG
    GATTATGGGGAGCATGCGAAAGAGTTACGAGCAAAACTGGACTTAATCCTTGGAGATACA
    GACAATCCACATAAAATTACAAATGAGCGTATAAAAAAAGAAATGATCGATGACTTAAAT
    TCAATTATAGATGATTTCTTTATGGAGACTAAACAAAATAGACCGAATTCTATAACAAAA
    TATGATCCAACAAAACACAATTTTAAAGAGAAGAGTGAAAATAAACCTAATTTTGATAAA
    TTAGTTGAAGAAACAAAAAAAGCAGTTAAAGAAGCAGACGAATCTTGGAAAAATAAAACT
    GTCAAAAAATACGAGGAAACTGTAACAAAATCTCCTGTTGTAAAAGAAGAGAAGAAAGTT
    GAAGAACCTCAATTACCTAAAGTTGGAAACCAGCAAGAGGTTAAAACTACGGCTGGTAAA
    GCTGAAGAAACAACACAACCAGTGGCACAGCCATTAGTAAAAATTCCACAAGAAACAATC
    TATGGTGAAACTGTAAAAGGTCCAGAATATCCAACGATGGAAAATAAAACGTTACAAGGT
    GAAATCGTTCAAGGTCCCGATTTTCTAACAATGGAACAAAACAGACCATCTTTAAGCGAT
    AATTATACTCAACCGACGACACCGAACCCTATTTTAGAAGGTCTTGAAGGTAGCTCATCT
    AAACTTGAAATAAAACCACAAGGTACTGAATCAACGTTGAAAGGTATTCAAGGAGAATCA
    AGTGATATTGAAGTTAAACCTCAAGCAACTGAAACAACAGAAGCTTCTCAATATGGTCCG
    AGACCGCAATTTAACAAAACACCTAAGTATGTGAAATATAGAGATGCTGGTACAGGTATC
    CGTGAATACAACGATGGAACATTTGGATATGAAGCGAGACCAAGATTCAACAAGCCAAGT
    GAAACAAATGCATACAACGTAACGACAAATCAAGATGGCACAGTATCATACGGAGCTCGC
    CCAACACAAAACAAGCCAAGTGAAACAAACGCATATAACGTAACAACACATGCAAATGGT
    CAAGTATCATACGGTGCTCGCCCAACACAAAAAAAGCCAAGCAAAACAAATGCATACAAC
    GTAACAACACATGCAAATGGTCAAGTATCATATGGCGCTCGCCCGACACAAAAAAAGCCA
    AGCAAAACAAATGCATATAACGTAACAACACATGCAAATGGTCAAGTATCATACGGAGCT
    CGCCCGACATACAAGAAGCCAAGCGAAACAAATGCATACAACGTAACAACACATGCAAAT
    GGTCAAGTATCATATGGCGCTCGCCCGACACAAAAAAAGCCAAGCGAAACAAACGCATAT
    AACGTAACAACACATGCAGATGGTACTGCGACATATGGGCCTAGAGTAACAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1977

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0222 [new locus tag: SA_RS01295 ]
  • symbol: Coa
  • description: staphylocoagulase
  • length: 658
  • theoretical pI: 8.74741
  • theoretical MW: 74519.3
  • GRAVY: -1.04301

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines mitochondrial import receptor subunit (TIGR00985; HMM-score: 13)
  • TheSEED  :
    • Staphylocoagulase
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Staphylocoagulase
  • PFAM:
    no clan defined Coagulase; Staphylococcus aureus coagulase (PF08764; HMM-score: 452)
    and 2 more
    Staphylcoagulse; Staphylocoagulase repeat (PF04022; HMM-score: 333.6)
    Ntox25; Bacterial toxin 25 (PF15530; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NKADAIVT
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.985782
    • TAT(Tat/SPI): 0.00152
    • LIPO(Sec/SPII): 0.001861
    • Cleavage Site: CS pos: 26-27. ADA-IV. Pr: 0.9147
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKQIISLGALAVASSLFTWDNKADAIVTKDYSKESRVNEKSKKGATVSDYYYWKIIDSLEAQFTGAIDLLEDYKYGDPIYKEAKDRLMTRVLGEDQYLLKKKIDEYELYKKWYKSSNKNTNMLTFHKYNLYNLTMNEYNDIFNSLKDAVYQFNKEVKEIEHKNVDLKQFDKDGEDKATKEVYDLVSEIDTLVVTYYADKDYGEHAKELRAKLDLILGDTDNPHKITNERIKKEMIDDLNSIIDDFFMETKQNRPNSITKYDPTKHNFKEKSENKPNFDKLVEETKKAVKEADESWKNKTVKKYEETVTKSPVVKEEKKVEEPQLPKVGNQQEVKTTAGKAEETTQPVAQPLVKIPQETIYGETVKGPEYPTMENKTLQGEIVQGPDFLTMEQNRPSLSDNYTQPTTPNPILEGLEGSSSKLEIKPQGTESTLKGIQGESSDIEVKPQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTNQDGTVSYGARPTQNKPSETNAYNVTTHANGQVSYGARPTQKKPSKTNAYNVTTHANGQVSYGARPTQKKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHANGQVSYGARPTQKKPSETNAYNVTTHADGTATYGPRVTK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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