PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00345 [old locus tag: SA0040 ]
• pan locus tag^?: SAUPAN000104000
• symbol: SA_RS00345
• pan gene symbol^?: mecI
• synonym:
• product: mecA-type methicillin resistance repressor MecI

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00345 [old locus tag: SA0040 ]
• symbol: SA_RS00345
• product: mecA-type methicillin resistance repressor MecI
• replicon: chromosome
• strand: +
• coordinates: 48894..49265
• length: 372
• essential: no DEG

⊟Accession numbers

• Location: NC_002745 (48894..49265) NCBI
• BioCyc: SA_RS00345 BioCyc
• MicrobesOnline: see SA0040

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  
  ATGGATAATAAAACGTATGAAATATCATCTGCAGAATGGGAAGTTATGAATATCATTTGG
  ATGAAAAAATATGCAAGTGCGAATAATATAATAGAAGAAATACAAATGCAAAAGGACTGG
  AGTCCAAAAACCATTCGTACACTTATAACGAGATTGTATAAAAAGGGATTTATAGATCGT
  AAAAAAGACAATAAAATTTTTCAATATTACTCTCTTGTAGAAGAAAGTGATATAAAATAT
  AAAACATCTAAAAACTTTATCAATAAAGTATACAAAGGCGGTTTCAATTCACTTGTCTTA
  AACTTTGTAGAAAAAGAAGATCTATCACAAGATGAAATAGAAGAATTGAGAAATATATTG
  AATAAAAAATAA
  60
  120
  180
  240
  300
  360
  372
  

⊟Protein

• 1OKR
  PDB
• 1SAX
  PDB
• 1SD6
  PDB
• 1SD7
  PDB
• 2D45
  PDB

Protein Data Bank: 1OKR

Protein Data Bank: 1SAX

Protein Data Bank: 1SD6

Protein Data Bank: 1SD7

Protein Data Bank: 2D45

⊟General

• locus tag: SA_RS00345 [old locus tag: SA0040 ]
• symbol: SA_RS00345
• description: mecA-type methicillin resistance repressor MecI
• length: 123
• theoretical pI: 9.46589
• theoretical MW: 14789.9
• GRAVY: -0.74065

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds copper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 102.1)
  Signal transduction Regulatory functions DNA interactions copper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 102.1)
  and 3 more
  Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 16.5)
  Signal transduction Regulatory functions DNA interactions phenylacetic acid degradation operon negative regulatory protein PaaX (TIGR02277; HMM-score: 16)
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 14.8)
• TheSEED: see SA0040
• PFAM:
  HTH (CL0123) Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 130.6)
  and 10 more
  MarR; MarR family (PF01047; HMM-score: 23.4)
  PaaX; PaaX-like protein (PF07848; HMM-score: 17.9)
  TFIIE_alpha; TFIIE alpha subunit (PF02002; HMM-score: 17.2)
  no clan defined DUF3489; Protein of unknown function (DUF3489) (PF11994; HMM-score: 15.2)
  SRP54_N; SRP54-type protein, helical bundle domain (PF02881; HMM-score: 14.7)
  HTH (CL0123) Cullin_Nedd8; Cullin protein neddylation domain (PF10557; HMM-score: 14.3)
  Sulfolobus_pRN; Sulfolobus plasmid regulatory protein (PF05584; HMM-score: 13.5)
  no clan defined DUF1160; Protein of unknown function (DUF1160) (PF06648; HMM-score: 13.2)
  post-AAA (CL0604) Rep_fac_C; Replication factor C C-terminal domain (PF08542; HMM-score: 12.7)
  HTH (CL0123) LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by MecI, TF important in Penicillin and methicillin resistance: see SA0040

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.009467
  • TAT(Tat/SPI): 0.000301
  • LIPO(Sec/SPII): 0.001842
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446291359 NCBI
• RefSeq: WP_000369214 NCBI
• UniProt: see SA0040

⊟Protein sequence

• MDNKTYEISSAEWEVMNIIWMKKYASANNIIEEIQMQKDWSPKTIRTLITRLYKKGFIDRKKDNKIFQYYSLVEESDIKYKTSKNFINKVYKGGFNSLVLNFVEKEDLSQDEIEELRNILNKK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: MecI see SA0040

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications