PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14365 [old locus tag: SAUSA300_2583 ]
• pan locus tag^?: SAUPAN006381000
• symbol: SAUSA300_RS14365
• pan gene symbol^?: gtfA
• synonym:
• product: accessory Sec system glycosyltransferase GtfA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14365 [old locus tag: SAUSA300_2583 ]
• symbol: SAUSA300_RS14365
• product: accessory Sec system glycosyltransferase GtfA
• replicon: chromosome
• strand: -
• coordinates: 2802061..2803569
• length: 1509
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2802061..2803569) NCBI
• BioCyc: SAUSA300_RS14365 BioCyc
• MicrobesOnline: see SAUSA300_2583

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  481
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  1021
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  1261
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  1441
  1501
  
  ATGACAATATATAATATAAACTTTGGTATAGGCTGGGCGAGTAGCGGTGTTGAATATGCA
  CAAGCTTACCGTGCTAAATTATTGAGACGTACAAATCAAGACACTAAATTTGTGTTTTTA
  GATTTTATACAGTCAGAGAACATACAGACATTAACGCAAAACATTGGGTTTAAAGATCAA
  GAAGTTATTTGGTTGTATCAGTATTTTAGTGATATTTCTTTAGCACCAACAACGTACACG
  TTAGATGACATTGAACGTGAGCTTGGTCATGACATTAGTTTTAGAGAAAGAAATGGTAAG
  ATAGTCCGCCTATATTTTAATGGGAAATCATCATTTGTAACATGTTATCTGCAAAATGAA
  CAAAAAGATATTGTGGATCGTGCAGAATTTGTAATTAATAGTATGTTAGTACGTAAAGAT
  TTTTATAGTTATACGCGCATATTTTCTGAGTATTATGCACCAGCTGATAATAAAGCGAAA
  TTATATATGCGACAATTTTATAATCAAGATGGTTCCATCGCTTATAACGAATATATTGAT
  GGAGACAGCTCTATATATGTGTTTGAAGATGCGGTGTTGTATAACAAAACGGAATTTATT
  GCATACTTTTTACAACGATTGAATTTAACCCGAGACGATATTGTCATATTAGACCGCGCT
  TCTGATATTGGTCAGGCTGTTTTGCAGCATAAAGGTGATAGTAAAGTTGGTGTTGTGATT
  CATGCTGATCATTATAGTAATAATATGATGAGTGAGCAGCATATACTATGGAACAATTAT
  TATGAATATCAATTTTCAAAAGCTAAATACATTGATTTCTTTATTACAGCAACAGATATT
  CAAAATCATATGGTTTGTCGACAATTTGAGCAATATCAAGGTTATCGCCCACGTGTTTAT
  ACTATTCCTGTGGGAAGCATTGATGCATTATCATATCCAACATTATCAAGAAAGCCATAT
  GCGATGATTAGTGCATCTCGTTTAGCGAATGAGAAACATATTGATTGGCTTGTCAAAGCG
  GTTATCGTGGCTAAACGACAAGTGCCAGAATTAACATTTGATATTTATGGAGAAGGTAGC
  GAGAAGACGCGACTGCGTAAAATTATTGATACACACCGCGCACAAGATTATATTCGTTTA
  CTAGGGCATGTTAAACTTGATGAAATTTATAATGATTATGAGTTATTTCTTTCTGCATCA
  ACTAGCGAAGGTTTTGGGTTAACACTAATGGAAGCGGTAGGCTCAGGACTAGGTATGATT
  GGATTAAATGTGAATTATGGTAACCCAACCTTTATTCGAGATGGGGAAAATGGCTACTTG
  GTACCTTTTGATACCGACGAAGATAGTGTGGATGATGTTATCGCAAAACTAGCTCATGCC
  ATTGTAATGTATTTTAACAATGGTCCCCAGACACCACATGACATCTCTTATGAAGTGGCA
  CAGCAATTTATGACTCAAGATATTATTTTAAAATGGGAAACATTAGTTCAAGAGGTGCTA
  CATGATTAA
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  1509
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS14365 [old locus tag: SAUSA300_2583 ]
• symbol: SAUSA300_RS14365
• description: accessory Sec system glycosyltransferase GtfA
• length: 502
• theoretical pI: 4.90817
• theoretical MW: 58272.2
• GRAVY: -0.311753

⊟Function

• reaction:
  EC 2.4.1.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 867)
  and 11 more
  sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 55.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 51.1)
  colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 36.9)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 21.8)
  glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 21)
  sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 19)
  D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 18.2)
  PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 16.8)
  Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 15.3)
  sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 11.7)
  putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 10.7)
• TheSEED: see SAUSA300_2583
• PFAM:
  no clan defined GtfA_EBD; GtfA extended beta-sheet domain (PF22145; HMM-score: 147)
  and 4 more
  GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 86.1)
  Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 80.5)
  no clan defined DUF5503; Family of unknown function (DUF5503) (PF17607; HMM-score: 15.5)
  GT-B (CL0113) GT4-conflict; Family 4 Glycosyltransferase in conflict systems (PF20706; HMM-score: 14.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9561
  • Cytoplasmic Membrane Score: 0.0214
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.0221
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014868
  • TAT(Tat/SPI): 0.000543
  • LIPO(Sec/SPII): 0.002457
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446080578 NCBI
• RefSeq: WP_000158433 NCBI
• UniProt: see SAUSA300_2583

⊟Protein sequence

• MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQSENIQTLTQNIGFKDQEVIWLYQYFSDISLAPTTYTLDDIERELGHDISFRERNGKIVRLYFNGKSSFVTCYLQNEQKDIVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAKLYMRQFYNQDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILDRASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFITATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHIDWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYNDYELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSVDDVIAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, JSNZ, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications