PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS14155 [old locus tag: SAUSA300_2549 ]
• pan locus tag^?: SAUPAN006318000
• symbol: SAUSA300_RS14155
• pan gene symbol^?: cudT
• synonym:
• product: choline transporter BetT

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS14155 [old locus tag: SAUSA300_2549 ]
• symbol: SAUSA300_RS14155
• product: choline transporter BetT
• replicon: chromosome
• strand: -
• coordinates: 2754711..2756333
• length: 1623
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2754711..2756333) NCBI
• BioCyc: SAUSA300_RS14155 BioCyc
• MicrobesOnline: see SAUSA300_2549

⊟Phenotype

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⊟DNA sequence

• 1
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  481
  541
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  721
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  841
  901
  961
  1021
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  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  
  TTGAAAAAAGAGAAAGTTATGGACTGGACGACCTTTATAGGGACAGTAGCTGTACTTCTT
  TTTGCAGTTATACCTATGATGGCTTTTCCAAAAGCAAGTGAAGATATCATCACTGGTATT
  AATAGTGCCATTTCTGATTCAATTGGTTCGATATATTTATTTATGGGGCTGGCGATTTTT
  TGCTTTGTAATGTATATCGCATTTGGTAAATATGGTAATGTCACACTTGGTAAAGCAAGT
  GATAAACCAGAATTTAATACATTTACATGGGCGGCAATGCTGTTTTGTGCAGGCATAGGC
  TCTGATATTTTATACTGGGGCGTTATTGAATGGGCTTTTTACTATCAAGTTCCACCAAAT
  GGCGCGAAAAGTATGAGTGATGAAGCACTCCAATATGCGACGCAATATGGTATGTTCCAC
  TGGGGGCCAATTGCTTGGGCTATTTATGTTCTACCAGCATTACCAATTGGTTATTTAGTA
  TTTGTTAAAAAACAACCGGTGTATAAAATTAGTCAAGCTTGTCGTCCGATTTTAAAAGGT
  CAAACAGATAAATTTGTAGGTAAAGTTGTAGATATCTTATTTATCTTTGGATTGCTAGGT
  GGTGCGGCAACATCACTAGCGTTAGGTGTGCCATTAATTTCTGCAGGCATAGAAAGATTA
  ACTGGTTTAGATGGTAAAAATATGATTTTACGTTCGGCCATTTTATTAACAATCACGGTT
  ATATTTGCCATTAGTTCATATACAGGATTGAAAAAAGGTATTCAAAAGTTAAGTGATATC
  AACGTTTGGCTATCCTTTGTACTTTTAGCCTTTATATTTATTATTGGACCGACTGTTTTT
  ATTATGGAAACGACAGTGACAGGGTTCGGAAATATGTTGAGAGATTTCTTTCATATGGCA
  ACATGGTTAGAACCATTCGGTGGTATTAAAGGTCGAAAAGAAACGAATTTCCCACAAGAC
  TGGACAATATTCTACTGGTCATGGTGGTTAGTATATGCGCCATTTATCGGTTTATTTATC
  GCTAGAATTTCAAAAGGTCGACGCCTTAAAGAAGTCGTGCTAGGAACAATTATTTATGGA
  ACGCTTGGATGCGTATTATTCTTTGGTATTTTTGGTAACTATGCTGTGTATTTACAAATT
  TCTGGACAGTTTAATGTAACACAATATTTAAATACACATGGTACAGAGGCAACCATTATT
  GAAGTGGTGCATCATTTACCATTCCCATCATTGATGATTGTACTATTCTTAGTATCTGCT
  TTCTTATTCTTAGCAACAACATTTGATTCGGGTTCATATATTTTAGCGGCAGCATCTCAG
  AAAAAAGTGGTAGGCGAACCATTACGTGCCAATCGTTTATTCTGGGCATTTGCATTGTGC
  TTATTGCCATTTTCATTGATGCTAGTTGGTGGTGAACGTGCATTAGAAGTATTGAAAACT
  GCTTCAATACTGGCAAGTGTGCCATTAATTGTTATTTTTATTTTCATGATGATATCATTT
  TTAATCATTTTAGGGCGCGATAGAATTAAACTTGAAACGCGTGCTGAAAAATTAAAAGAA
  GTTGAACGTCGTTCATTGCGAATCGTTCAAGTATCAGAAGAAGAACAAGACGATAATTTA
  TAA
  60
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  1140
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  1260
  1320
  1380
  1440
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  1560
  1620
  1623
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS14155 [old locus tag: SAUSA300_2549 ]
• symbol: SAUSA300_RS14155
• description: choline transporter BetT
• length: 540
• theoretical pI: 9.09649
• theoretical MW: 60254
• GRAVY: 0.619259

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Other transporter, betaine/carnitine/choline transporter (BCCT) family (TIGR00842; HMM-score: 460.9)
• TheSEED: see SAUSA300_2549
• PFAM:
  APC (CL0062) BCCT; BCCT, betaine/carnitine/choline family transporter (PF02028; HMM-score: 507.5)
  and 2 more
  no clan defined AtTam38; AtTam38 family (PF25114; HMM-score: 15.2)
  PH (CL0266) DUF3093; Protein of unknown function (DUF3093) (PF11292; HMM-score: 13.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 1
  • Cell wall & surface Score: 0
  • Extracellular Score: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.242236
  • TAT(Tat/SPI): 0.005112
  • LIPO(Sec/SPII): 0.056956
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 446634524 NCBI
• RefSeq: WP_000711870 NCBI
• UniProt: see SAUSA300_2549

⊟Protein sequence

• MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAISDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSDILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLVFVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIERLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIGPTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAPFIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNTHGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRANRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDRIKLETRAEKLKEVERRSLRIVQVSEEEQDDNL

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: ArgR/AhrC see SAUSA300_2549

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications