From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS13750 [old locus tag: SAUSA300_2478 ]
  • pan locus tag?: SAUPAN006197000
  • symbol: SAUSA300_RS13750
  • pan gene symbol?: cidB
  • synonym:
  • product: holin-like protein CidB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS13750 [old locus tag: SAUSA300_2478 ]
  • symbol: SAUSA300_RS13750
  • product: holin-like protein CidB
  • replicon: chromosome
  • strand: -
  • coordinates: 2676429..2677118
  • length: 690
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATGATTACGTGCAAGCCTTATTAATGATTTTGTTGACTGTCGTTTTATATTATTTC
    GCTAAAAGGTTACAACAAAAATATCCGAACCCATTTTTGAATCCAGCATTAATTGCATCT
    TTAGGAATTATTTTTGTCTTACTTATCTTTGGAATTAGTTATAACGGGTATATGAAAGGT
    GGCAGTTGGATCAACCATATTTTAAACGCAACGGTCGTATGTTTAGCGTACCCACTTTAT
    AAAAATAGAGAGAAAATTAAAGACAATGTCTCTATCATTTTTGCAAGTGTATTAACTGGC
    GTCATGCTGAATTTCATGTTAGTGTTCTTAACACTTAAAGCATTTGGCTATTCTAAAGAC
    GTCATTGTAACGTTATTGCCCCGATCTATAACAGCCGCAGTAGGTATCGAAGTGTCACAT
    GAACTAGGTGGTACAGATACGATGACCGTACTTTTTATTATCACAACGGGTTTAATCGGT
    AGTATTTTAGGTTCGATGTTATTAAGATTTGGAAGATTTGAATCTTCTATCGCCAAAGGA
    TTAACGTATGGGAATGCGTCACATGCATTTGGCACAGCTAAAGCACTAGAAATGGATATT
    GAATCCGGTGCATTTAGTTCAATTGGGATGATTTTAACTGCAGTTATTAGTTCAGTGTTA
    ATACCTGTTCTAATTTTATTATTCTATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    690

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS13750 [old locus tag: SAUSA300_2478 ]
  • symbol: SAUSA300_RS13750
  • description: holin-like protein CidB
  • length: 229
  • theoretical pI: 9.40732
  • theoretical MW: 25011.7
  • GRAVY: 0.851528

⊟Function[edit | edit source]

  • TIGRFAM:
    Hypothetical proteins Conserved TIGR00659 family protein (TIGR00659; HMM-score: 192.1)
  • TheSEED: see SAUSA300_2478
  • PFAM:
    no clan defined LrgB; LrgB-like family (PF04172; HMM-score: 232.6)
    and 4 more
    RETICULATA-like; Protein RETICULATA-related (PF11891; HMM-score: 12.8)
    DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 10.7)
    Thioredoxin (CL0172) OST3_OST6; OST3 / OST6 family, transporter family (PF04756; HMM-score: 7.6)
    no clan defined DUF3671; Protein of unknown function (PF12420; HMM-score: 7.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002069
    • TAT(Tat/SPI): 0.000164
    • LIPO(Sec/SPII): 0.006048
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGIIFVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVMLNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIGSILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISSVLIPVLILLFY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_RS13750&oldid=91880"