Jump to navigation
Jump to search
NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS12210 [old locus tag: SAUSA300_2213 ]
- pan locus tag?: SAUPAN005717000
- symbol: SAUSA300_RS12210
- pan gene symbol?: rnd2
- synonym:
- product: AcrB/AcrD/AcrF family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS12210 [old locus tag: SAUSA300_2213 ]
- symbol: SAUSA300_RS12210
- product: AcrB/AcrD/AcrF family protein
- replicon: chromosome
- strand: -
- coordinates: 2380364..2383531
- length: 3168
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2380364..2383531) NCBI
- BioCyc: SAUSA300_RS12210 BioCyc
- MicrobesOnline: see SAUSA300_2213
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121GTGATAAAAAAGCTATTACAATTTTCTTTAGGGAATAAGTTTGCTATCTTTTTAATGGTT
GTTTTAGTTGTCTTGGGCGGTGTATATGCGAGTGCTAAATTGAAATTAGAATTACTACCA
AATGTACAAAATCCAGTTATTTCAGTTACAACAACAATGCCGGGTGCAACGCCACAAAGT
ACCCAAGATGAAATAAGTAGTAAAATTGACAATCAAGTAAGATCATTGGCATATGTGAAA
AATGTTAAAACGCAATCCATACAAAATGCTTCAATTGTAACAGTTGAATATGAAAATAAT
ACAGATATGGATAAAGCAGAAGAACAGCTTAAAAAAGAAATCGATAAAATTAAATTTAAA
GATGAAGTTGGTCAACCAGAATTAAGACGTAATTCGATGGATGCTTTTCCGGTTTTAGCA
TATTCATTTTCAAATAAAGAGAATGACTTGAAAAAAGTAACGAAAGTACTGAATGAACAA
TTAATACCAAAATTGCAAACGGTAGATGGTGTGCAAAATGCGCAATTAAATGGGCAGACG
AACCGTGAAATCACCCTTAAATTTAAGCAAAATGAACTTGAAAAATATGGGTTGACTGCT
GATGATGTAGAAAACTATCTAAAAACGGCAACAAGAACAACGCCACTTGGATTGTTCCAA
TTTGGTGATAAAGATAAATCAATTGTTGTTGATGGTCAATATCAATCTGTTGATGCTTTT
AAAAACATAAATATTCCATTAACGTTGGCAGGAGGACAAGGGCAATCTCAATCCCAAAGT
GACAATAAACAAAATTCAGCCATGTCAGACGTTAATCAGGCATCACCACAGCAAAATTCA
AAAGCGTCAGCACCAAATAATATAAGTGGTATGCCGACAGCGAAACTAGGAGATTTAGCT
GATATTACAGTTGGTGATGTGCGTACTTCTATTTCTAAAACGAATGGAAAGGATGCGGTT
AATCTACAAATAACTAAAGCTCAAGATGCCAATACAGTTCAAGTAGCCAAAGATGTACAA
CGTAAAATTGATACATTCGTTGATGAAAATAAAGATTTTAATGTCACAAAAACAATGGAT
ACTGCAAAGCCTGTTGAGAAATCACTTTATACGATGGTTGAAAAAGCATCATTAGGTACA
ATCGTGGCAATTATAGTTATTTTGCTGTTTTTAAGAAACATTCGTACGACGGCAATTTCT
ATTATATCGATTCCGTTATCACTTCTTATGGCGCTTATTGCTCTGAAATTGAGTGATGTT
TCATTGAATATACTAACGTTAGGTGCATTAACAGTAGCGATTGGACGTGTGATAGACGAT
TCGATTGTAGTTGTTGAAAATATTTATCGACGCTTAACAGATTCAGAAGAACAACTAAAA
GGTGAAAATTTAATTATCAGTGCGACAACTGAAGTATTTAAACCAATAATGTCATCGACA
CTAGTTACTATTATCGTCTTCTTACCACTTGTGTTTGTATCAGGTTCAGTAGGCGAAATG
TTTAGACCTTTTGCATTGGCTATTGCATTTAGTTTATTAGCATCGTTATTAGTGTCAATT
ACACTCGTTCCAGCGTTGGCAGCTACACTATTTAAAAAAGGCGTTAAACGTCGTAATAAA
CAACATCAAGAAGGATTAGGTGTTGTTAGTACAACTTATAAAAAAGTATTACATTGGTCA
TTAAATCATAAGTGGATTGTAATTATATTAAGTACATTAATTTTGGTTGCAACTATTGTA
TTTGGAGGACCGAGACTAGGCACTAGCTTTATTTCAGCAGGTGACGATAAATTTTTAGCT
ATTACTTATACACCGAAGCCTGGTGAAACGGAGCAAGCAGTGTTGAATCATGCGAAAGAT
GTTGAAAAATATTTAAAACAGAAAAAGCATGTAAAAACAATTCAATACTCAGTTGGCGGT
AGTAGTCCAGTAGATCCAACGGGTAGTACAAATAGTATGGCAATCATGGTTGAATATGAT
AATGACACGCCTAATTTTGATGTAGAAGCGGATAAGGTTATTAAACATGCAGATGGCTTT
AAACATCCTGGAGAGTGGAAAAATCAAGATTTAGGAACAGGTGCAGGTAATAAATCTGTA
GAGGTTACTGTAAAAGGTCCATCAATGGATGCCATAAAATCAACTGTAAAAGATATTGAA
CAGAAAATGAAACAGGTTAAAGGACTAGCCAATGTCAAATCTGATTTATCGCAAACATAT
GATCAGTATGAAATTAAAGTCGATCAAAATAAAGCGGCAGAAAATGGTATTTCTGCAAGT
CAACTTGCAATGCACTTGAATGAAAACTTACCAGAAAAAACAGTTACGACTGTTAAAGAA
AATGGTAAAACTGTTGATGTTAAAGTCAAACAAAATAAGCAAACAGACTGGTCAGAAGAT
AAGTTGAATAATATAACTTTGAAAAAGCCGACTGGTGGTACGATTAAATTGGGAGATATC
GCTACGTTAGTTAAAACAACGACACCAAGTAAATTGACGCAAGAACAAGGAGATTATGCA
ACGACGGTATCTGCTAAAGTAACAAATAAAGATGTGGGTGGCACAACACGACAAGTGATG
TCTAAAATAAATAATTTGGACAAACCGAATAATGTAAAGGTTAATATCGGTGGTGCATCA
GATGATATTAACAATGCAATGACACAATTAGCCTTTGCAATGTTAGCTGCAATCATTATC
GTATATTTAATCCTAGTTATTACATTTAAAGGTGGCCTAGCACCATTTACAATTTTATTC
TCTTTACCATTTACAGTTATCGGTGTAATTATTGCACTATTAATCACAGGAGAAACAATA
TCAGTACCAAGTTTAATTGGTATGCTAATGTTAATTGGAATCGTAGTAACAAATGCCATT
GTGTTAATAGACCGTGTTATTAATAATGAGCAACAGGGCATGGAGATGAAAGAAGCATTA
ATCGAAGCAGGCGGTACTAGAATTAGACCGATATTAATGACGGCGATTGCAACAATTGGT
GCATTAGTTCCTTTGTTATTTGGTCAAGATAGCTCGATTCTTATTTCGAAAGGATTAGCT
GCTACAGTTATTGGTGGTTTAATTTCATCAACTTTATTAACACTTGTAGTTGTACCAGTA
ATATATGAAATTCTCTTTACTTTAAAAAAACGATTCACTAAACGATAG60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1020
1080
1140
1200
1260
1320
1380
1440
1500
1560
1620
1680
1740
1800
1860
1920
1980
2040
2100
2160
2220
2280
2340
2400
2460
2520
2580
2640
2700
2760
2820
2880
2940
3000
3060
3120
3168
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS12210 [old locus tag: SAUSA300_2213 ]
- symbol: SAUSA300_RS12210
- description: AcrB/AcrD/AcrF family protein
- length: 1055
- theoretical pI: 9.68801
- theoretical MW: 114701
- GRAVY: 0.0117536
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Detoxification heavy metal efflux pump, CzcA family (TIGR00914; HMM-score: 405.6)Transport and binding proteins Cations and iron carrying compounds heavy metal efflux pump, CzcA family (TIGR00914; HMM-score: 405.6)Cellular processes Toxin production and resistance RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family (TIGR00915; HMM-score: 362.3)Transport and binding proteins Unknown substrate RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family (TIGR00915; HMM-score: 362.3)and 5 moreProtein fate Protein and peptide secretion and trafficking protein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 47.9)Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecF (TIGR00966; HMM-score: 44.2)Transport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 34.2)hopanoid biosynthesis associated RND transporter like protein HpnN (TIGR03480; HMM-score: 26.3)Mobile and extrachromosomal element functions Prophage functions phage minor tail protein G (TIGR01674; HMM-score: 16.8)
- TheSEED: see SAUSA300_2213
- PFAM: RND_permease (CL0322) ACR_tran; AcrB/AcrD/AcrF family (PF00873; HMM-score: 675)and 4 moreMMPL; MMPL family (PF03176; HMM-score: 52.6)SecD_SecF; Protein export membrane protein (PF02355; HMM-score: 50.6)Oxa1 (CL0376) DUF106; Integral membrane protein DUF106 (PF01956; HMM-score: 8.9)no clan defined Orf78; Orf78 (ac78) (PF06024; HMM-score: 8.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 10
- LocateP:
- SignalP: Signal peptide SP(Sec/SPI) length 32 aa
- SP(Sec/SPI): 0.802545
- TAT(Tat/SPI): 0.004497
- LIPO(Sec/SPII): 0.022605
- Cleavage Site: CS pos: 32-33. ASA-KL. Pr: 0.4027
- predicted transmembrane helices (TMHMM): 12
⊟Accession numbers[edit | edit source]
- GI: 446514957 NCBI
- RefSeq: WP_000592303 NCBI
- UniProt: see SAUSA300_2213
⊟Protein sequence[edit | edit source]
- MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQNPVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTDMDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQLIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGLFQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASPQQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQVAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLRNIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYRRLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAIAFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWIVIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKYLKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKHPGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTYDQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWSEDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTTRQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLAPFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQGMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISSTLLTLVVVPVIYEILFTLKKRFTKR
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.