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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02525
  • pan locus tag?: SAUPAN005717000
  • symbol: SAOUHSC_02525
  • pan gene symbol?: rnd2
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02525
  • symbol: SAOUHSC_02525
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2327150..2330317
  • length: 3168
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    GTGATAAAAAAGCTATTACAATTTTCTTTAGGGAATAAGTTTGCTATCTTTTTAATGGTT
    GTTTTAGTTGTCTTGGGCGGTGTATATGCGAGTGCTAAATTGAAATTAGAATTACTACCA
    AATGTACAAAATCCAGTTATTTCAGTTACAACAACAATGCCGGGTGCAACGCCACAAAGT
    ACCCAAGATGAAATAAGTAGTAAAATTGACAATCAAGTAAGATCATTGGCATATGTGAAA
    AATGTTAAAACGCAATCCATACAAAATGCTTCAATTGTAACAGTTGAATATGAAAATAAT
    ACAGATATGGATAAAGCAGAAGAACAGCTTAAAAAAGAAATCGATAAAATTAAATTTAAA
    GATGAAGTTGGTCAACCAGAATTAAGACGTAATTCGATGGATGCTTTTCCGGTTTTAGCA
    TATTCATTTTCAAATAAAGAGAATGACTTGAAAAAAGTAACGAAAGTACTGAATGAACAA
    TTAATACCAAAATTGCAAACGGTAGATGGTGTGCAAAATGCGCAATTAAATGGGCAGACG
    AACCGTGAAATCACCCTTAAATTTAAGCAAAATGAACTTGAAAAATATGGGTTGACTGCT
    GATGATGTAGAAAACTATCTAAAAACGGCAACAAGAACAACGCCACTTGGATTGTTCCAA
    TTTGGTGATAAAGATAAATCAATTGTTGTTGATGGTCAATATCAATCTGTTGATGCTTTT
    AAAAACATAAATATTCCATTAACGTTGGCAGGAGGACAAGGGCAATCTCAATCCCAAAGT
    GACAATAAACAAAATTCAGCCATGTCAGACGTTAATCAGGCATCACCACAGCAAAATTCA
    AAAGCGTCAGCACCAAATAATATAAGTGGTATGCCGACAGCGAAACTAGGAGATTTAGCT
    GATATTACAGTTGGTGATGTGCGTACTTCTATTTCTAAAACGAATGGAAAGGATGCGGTT
    AATCTACAAATAACTAAAGCTCAAGATGCCAATACAGTTCAAGTAGCCAAAGATGTACAA
    CGTAAAATTGATACATTCGTTGATGAAAATAAAGATTTTAATGTCACAAAAACAATGGAT
    ACTGCAAAGCCTGTTGAGAAATCACTTTATACGATGGTTGAAAAAGCATCATTAGGTACA
    ATCGTGGCAATTATAGTTATTTTGCTGTTTTTAAGAAACATTCGTACGACGGCAATTTCT
    ATTATATCGATTCCGTTATCACTTCTTATGGCGCTTATTGCTCTGAAATTGAGTGATGTT
    TCATTGAATATACTAACGTTAGGTGCATTAACAGTAGCGATTGGACGTGTGATAGACGAT
    TCGATTGTAGTTGTTGAAAATATTTATCGACGCTTAACAGATTCAGAAGAACAACTAAAA
    GGTGAAAATTTAATTATCAGTGCGACAACTGAAGTATTTAAACCAATAATGTCATCGACA
    CTAGTTACTATTATCGTCTTCTTACCACTTGTGTTTGTATCAGGTTCAGTAGGCGAAATG
    TTTAGACCTTTTGCATTGGCTATTGCATTTAGTTTATTAGCATCGTTATTAGTGTCAATT
    ACACTCGTTCCAGCGTTGGCAGCTACACTATTTAAAAAAGGCGTTAAACGTCGTAATAAA
    CAACATCAAGAAGGATTAGGTGTTGTTAGTACAACTTATAAAAAAGTATTACATTGGTCA
    TTAAATCATAAGTGGATTGTAATTATATTAAGTACATTAATTTTGGTTGCAACTATTGTA
    TTTGGAGGACCGAGACTAGGCACTAGCTTTATTTCAGCAGGTGACGATAAATTTTTAGCT
    ATTACTTATACACCGAAGCCTGGTGAAACGGAGCAAGCAGTGTTGAATCATGCGAAAGAT
    GTTGAAAAATATTTAAAACAGAAAAAGCATGTAAAAACAATTCAATACTCAGTTGGCGGT
    AGTAGTCCAGTAGATCCAACGGGTAGTACAAATAGTATGGCAATCATGGTTGAATATGAT
    AATGACACGCCTAATTTTGATGTAGAAGCGGATAAGGTTATTAAACATGCAGATGGCTTT
    AAACATCCTGGAGAGTGGAAAAATCAAGATTTAGGAACAGGTGCAGGTAATAAATCTGTA
    GAGGTTACTGTAAAAGGTCCATCAATGGATGCCATAAAATCAACTGTAAAAGATATTGAA
    CAGAAAATGAAACAGGTTAAAGGACTAGCCAATGTCAAATCTGATTTATCGCAAACATAT
    GATCAGTATGAAATTAAAGTCGATCAAAATAAAGCGGCAGAAAATGGTATTTCTGCAAGT
    CAACTTGCAATGCACTTGAATGAAAACTTACCAGAAAAAACAGTTACGACTGTTAAAGAA
    AATGGTAAAACTGTTGATGTTAAAGTCAAACAAAATAAGCAAACAGACTGGTCAGAAGAT
    AAGTTGAATAATATAACTTTGAAAAAGCCGACTGGTGGTACGATTAAATTGGGAGATATC
    GCTACGTTAGTTAAAACAACGACACCAAGTAAATTGACGCAAGAACAAGGAGATTATGCA
    ACGACGGTATCTGCTAAAGTAACAAATAAAGATGTGGGTGGCACAACACGACAAGTGATG
    TCTAAAATAAATAATTTGGATAAACCGAATAATGTAAAGGTTAATATCGGTGGTGCATCA
    GATGATATTAACAATGCAATGACACAATTAGCCTTTGCAATGTTAGCTGCAATCATTATC
    GTATATTTAATCCTAGTTATTACATTTAAAGGTGGCCTAGCACCATTTACAATTTTATTC
    TCTTTACCATTTACAGTTATCGGTGTAATTATTGCACTATTAATCACAGGAGAAACAATA
    TCAGTACCAAGTTTAATTGGTATGCTAATGTTAATTGGAATCGTAGTAACAAATGCCATT
    GTGTTAATAGACCGTGTTATTAATAATGAGCAACAGGGCATGGAGATGAAAGAAGCATTA
    ATCGAAGCAGGCGGTACTAGAATTAGACCGATATTAATGACGGCGATTGCAACAATTGGT
    GCATTAGTTCCTTTGTTATTTGGTCAAGATAGCTCGATTCTTATTTCGAAAGGATTAGCT
    GCTACAGTTATTGGTGGTTTAATTTCATCAACTTTATTAACACTTGTAGTTGTACCAGTA
    ATATATGAAATTCTCTTTACTTTAAAAAAACGATTCACTAAACGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3168

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02525
  • symbol: SAOUHSC_02525
  • description: hypothetical protein
  • length: 1055
  • theoretical pI: 9.68801
  • theoretical MW: 114701
  • GRAVY: 0.0117536

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification heavy metal efflux pump, CzcA family (TIGR00914; HMM-score: 405.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds heavy metal efflux pump, CzcA family (TIGR00914; HMM-score: 405.6)
    Cellular processes Cellular processes Toxin production and resistance RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family (TIGR00915; HMM-score: 362.3)
    Metabolism Transport and binding proteins Unknown substrate RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family (TIGR00915; HMM-score: 362.3)
    and 5 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 47.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking protein-export membrane protein SecF (TIGR00966; HMM-score: 44.2)
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 34.2)
    hopanoid biosynthesis associated RND transporter like protein HpnN (TIGR03480; HMM-score: 26.3)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage minor tail protein G (TIGR01674; HMM-score: 16.8)
  • TheSEED  :
    • Acriflavin resistance protein
    • RND multidrug efflux transporter
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Multidrug Resistance Efflux Pumps  Acriflavin resistance protein
  • PFAM:
    RND_permease (CL0322) ACR_tran; AcrB/AcrD/AcrF family (PF00873; HMM-score: 675)
    and 4 more
    MMPL; MMPL family (PF03176; HMM-score: 52.6)
    SecD_SecF; Protein export membrane protein (PF02355; HMM-score: 50.6)
    Oxa1 (CL0376) DUF106; Integral membrane protein DUF106 (PF01956; HMM-score: 8.9)
    no clan defined Orf78; Orf78 (ac78) (PF06024; HMM-score: 8.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 32 aa
    • SP(Sec/SPI): 0.802545
    • TAT(Tat/SPI): 0.004497
    • LIPO(Sec/SPII): 0.022605
    • Cleavage Site: CS pos: 32-33. ASA-KL. Pr: 0.4027
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQNPVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTDMDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQLIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGLFQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASPQQNSKASAPNNISGMPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQVAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLRNIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYRRLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAIAFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWIVIILSTLILVATIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKYLKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKHPGEWKNQDLGTGAGNKSVEVTVKGPSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTYDQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWSEDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAKVTNKDVGGTTRQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLAPFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQGMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISSTLLTLVVVPVIYEILFTLKKRFTKR

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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