PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS11845 [old locus tag: SAUSA300_2150 ]
• pan locus tag^?: SAUPAN005586000
• symbol: SAUSA300_RS11845
• pan gene symbol^?: lacE
• synonym:
• product: PTS lactose EIICB component

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS11845 [old locus tag: SAUSA300_2150 ]
• symbol: SAUSA300_RS11845
• product: PTS lactose EIICB component
• replicon: chromosome
• strand: -
• coordinates: 2327801..2329513
• length: 1713
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2327801..2329513) NCBI
• BioCyc: SAUSA300_RS11845 BioCyc
• MicrobesOnline: see SAUSA300_2150

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATGCAAAAATTGATCGCACAAATTGAAAAAGGGAAGCCATTTTTCGAGAAATTATCA
  CGTAACATATATTTAAGAGCCATCCGTGATGGATTTATTTCTGCAATGCCTGTCATCTTA
  TTCTCAAGTATCTTTCTATTAATTGCATATGTGCCAAATATATTTGGTTTTAAATGGGAT
  AAAGGCATGGAAGCCATTTTAATGAAACCATACAACTATACGATGGGGCTTGTAGCATTT
  TTAGTAGCAGGAACGACAGCGAAATCTTTAACAGATTCATTTAACCGTAAGCTAGAAAGT
  ACAAATCAAATTAACTTTATTTCTACAATGCTAGCAGCGATGTGTGGATTCTTGTTCTTA
  GCTTCTGATCCAGCCAAAGATGGTGGATTCTTAAGTGCATTTATGGGAACTAAAGGTTTA
  TTAACAGCATTCTTATCAGCATTTGTAACTGTTATTGTTTATAACTTCTGTGTGAAGCGC
  AATATTACAATTAAAATGCCGAAAGAAGTACCGCCGAATATTTCACAAGTATTTAAGGAC
  TTAATTCCATTTTCAGCGGTAATCATCATTCTTTATGCATTAGATTTAGTCATTCGCAAC
  AGCTTTAAATCAAATGTAGCGGAAGGTATTTTAAAATTATTCGAACCATTATTTACAGCA
  GCAGATGGATGGATTGGTGTCACAATTATCTTTGGTGCCTTTGCATTATTCTGGTTTGTA
  GGTATTCATGGTCCGTCAATTGTAGAGCCAGCAATTGCAGCCATTACATATGCGAATATC
  GAAGCGAACTTCAAGTTGCTTCAAGCTGGAGAACACGCAGATAAAATTATTACATCAGGT
  ACACAAATGTTTATCGTTACATTTGGAGGTACGGGTGCAACATTGGTCGTCCCGTTCATG
  TTTATGTGGATGACGAAATCTAAACGAAACAAAGCGATTGGACGTGCATCAGTTGTACCA
  ACATTCTTTGGTGTAAACGAACCAATTTTATTTGGTGCACCACTAGTATTGAATCCTGTG
  TTCTTTATTCCATTTGTATTAGCACCAATTGTTAACGTATGGATTTTCAAACTTTTCGTT
  GAAGTGTTAGGAATGAATAGTTTTAGTGTGAATTTACCTTGGACAACACCAGGTCCATTG
  GGCATTATCATGGGTACAGGTTTTGGTTTATGGTCATTCGTACTAGCTATTACTTTGATT
  GTTGTAGATATTATTATTTACTACCCATTCCTAAAAGTTTATGATAGTGAAATTCTTGAT
  GAAGAAGAAGGACGTAAAGAAAGTAATTCAGATTTAAAAGAAAAAGTTGCAGCAAACTTT
  GATACGAAAAAAGCTGATTCAATTTTAGCGGCAAGTGGTGTATCAGACGATGCAGCTAAA
  GCTTCAAATATCACAGAACAAACAAATGTCTTAGTACTTTGTGCCGGTGGTGGTACAAGT
  GGATTATTAGCCAATGCACTTAATAAAGCAGCTGAAGAATATCATGTGCCAGTGAAAGCG
  GCAGCTGGCGGATATGGCGCACATATGGACATTATGAAAGAGTACCAATTAATCATTTTA
  GCGCCACAAGTTGCGTCTAACTATGAAGATATTAAGCAAGATACAGATAGATTAGGTATT
  AAATTAGCGAAGACTCAAGGTGCTGAATATATCAAATTGACACGAGATGGCCAAGCAGCC
  CTTGATTTCGTTCAACAACAATTTGAAAATTAA
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  1713
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS11845 [old locus tag: SAUSA300_2150 ]
• symbol: SAUSA300_RS11845
• description: PTS lactose EIICB component
• length: 570
• theoretical pI: 7.48602
• theoretical MW: 62431.8
• GRAVY: 0.442456

⊟Function

• reaction:
  EC 2.7.1.69?  ExPASy
  Transferred entry: 2.7.1.191, 2.7.1.192, 2.7.1.193, 2.7.1.194, 2.7.1.195, 2.7.1.196, 2.7.1.197, 2.7.1.198, 2.7.1.199, 2.7.1.200, 2.7.1.201, 2.7.1.202, 2.7.1.203, 2.7.1.204, 2.7.1.205, 2.7.1.206, 2.7.1.207 and 2.7.1.208
• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
  Signal transduction Signal transduction PTS PTS system, lactose-specific IIC component (TIGR00394; EC 2.7.1.69; HMM-score: 771.4)
  and 8 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
  Signal transduction Signal transduction PTS PTS system, cellobiose-specific IIC component (TIGR00359; EC 2.7.1.69; HMM-score: 267.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
  Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIC component (TIGR00410; EC 2.7.1.69; HMM-score: 267.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.3)
  Signal transduction Signal transduction PTS PTS system, lactose/cellobiose family IIB component (TIGR00853; HMM-score: 205.3)
  PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 35.2)
  PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 26.3)
• TheSEED: see SAUSA300_2150
• PFAM:
  PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 277.3)
  and 2 more
  Phosphatase (CL0031) PTS_IIB; PTS system, Lactose/Cellobiose specific IIB subunit (PF02302; HMM-score: 38.9)
  PFK (CL0240) DAGK_cat; Diacylglycerol kinase catalytic domain (PF00781; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9992
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0007
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001663
  • TAT(Tat/SPI): 0.000132
  • LIPO(Sec/SPII): 0.00121
• predicted transmembrane helices (TMHMM): 10

⊟Accession numbers

• GI: see SAUSA300_2150
• RefSeq: WP_000983330 NCBI
• UniProt: see SAUSA300_2150

⊟Protein sequence

• MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSIFLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTNQINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKRNITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLFTAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKIITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAPLVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWSFVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILAASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAHMDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQQFEN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for JSNZ, N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications