From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS11345 [old locus tag: SAUSA300_2060 ]
  • pan locus tag?: SAUPAN005396000
  • symbol: SAUSA300_RS11345
  • pan gene symbol?: atpA
  • synonym:
  • product: ATP synthase subunit alpha

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS11345 [old locus tag: SAUSA300_2060 ]
  • symbol: SAUSA300_RS11345
  • product: ATP synthase subunit alpha
  • replicon: chromosome
  • strand: -
  • coordinates: 2223272..2224780
  • length: 1509
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGCCATAAAAGCTGAAGAAATCAGTGCATTACTTCGCTCACAAATTGAAAATTATGAG
    TCAGAAATGTCCGTAACTGATGTAGGTACTGTATTACAAATTGGTGATGGTATTGCATTA
    ATTCACGGATTAAATGACGTTATGGCTGGTGAGCTAGTAGAATTCCATAACGGCGTACTT
    GGTTTAGCCCAAAACCTTGAAGAGTCAAACGTGGGTGTGGTTATTTTAGGACCATACACA
    GGTATTACTGAAGGTGACGAAGTTAAACGTACTGGTCGTATCATGGAAGTACCAGTAGGT
    GAAGAACTAATCGGAAGAGTTGTTAATCCATTAGGACAACCTATTGATGGACAAGGACCG
    ATTAACACAACTAAAACACGTCCAGTAGAGAAAAAAGCTACTGGTGTAATGGATCGTAAA
    TCAGTAGATGAGCCATTACAAACAGGTATCAAAGCAATTGATGCTTTAGTACCAATTGGT
    AGAGGTCAACGTGAGTTAATCATCGGTGACCGTCAAACAGGTAAAACAACAATTGCAATT
    GACACAATTTTGAACCAAAAAGATCAAGGTACGATTTGTATCTATGTTGCTATTGGTCAA
    AAAGATTCAACAGTAAGAGCAAATGTTGAAAAGTTAAGACAAGCAGGCGCTTTAGACTAC
    ACTATTGTTGTAGCAGCATCAGCTTCTGAACCTTCTCCATTATTATATATTGCACCATAT
    TCAGGTGTAACAATGGGTGAAGAATTCATGTTTAACGGTAAACATGTTTTAATCGTTTAT
    GATGATTTAACTAAACAAGCAGCAGCTTATCGTGAGTTATCATTATTATTACGTAGACCT
    CCAGGTCGTGAAGCATACCCAGGTGACGTATTCTACTTACATAGTAGATTATTAGAAAGA
    GCAGCAAAATTAAACGATGACTTAGGTGGCGGTTCAATTACTGCATTACCAATTATCGAA
    ACACAAGCTGGTGATATTTCAGCTTATGTACCAACAAACGTTATTTCAATTACAGATGGA
    CAAATCTTCTTACAATCTGATTTATTCTTCTCAGGTGTAAGACCAGCGATTAATGCCGGA
    CAATCTGTATCTCGTGTTGGTGGATCTGCACAAATTAAAGCAATGAAGAAAGTTGCTGGT
    ACGTTACGTCTTGACTTAGCGTCATACAGAGAACTTGAATCATTTGCACAATTCGGTTCA
    GACCTTGATGAATTTACTGCAAGTAAATTAGAACGTGGTAAACGTACTGTTGAAGTCTTA
    AAACAAGATCAAAACAAACCATTACCAGTCGAACACCAAGTGTTGATTATTTATGCATTA
    ACAAAAGGATATTTAGATGATATTCCAGTTGTAGATATCACACGTTTTGAAGACGAGTTA
    AACCACTGGGCAGAATCAAATGCTACTGAACTGTTAAATGAAATCAGAGAAACTGGTGGC
    TTACCAGATGCTGAGAAGTTTGACACAGCAATTAACGAATTCAAAAAAAGCTTTAGCAAA
    TCTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1509

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS11345 [old locus tag: SAUSA300_2060 ]
  • symbol: SAUSA300_RS11345
  • description: ATP synthase subunit alpha
  • length: 502
  • theoretical pI: 4.62947
  • theoretical MW: 54583.6
  • GRAVY: -0.147809

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.3.14?  ExPASy
    H+-transporting two-sector ATPase ATP + H2O + H+(In) = ADP + phosphate + H+(Out)
  • TIGRFAM:
    Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, alpha subunit (TIGR00962; EC 3.6.3.14; HMM-score: 836.6)
    and 17 more
    alternate F1F0 ATPase, F1 subunit alpha (TIGR03324; EC 3.6.3.-; HMM-score: 648.9)
    Cellular processes Cellular processes Pathogenesis type III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 205.7)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 205.7)
    Cellular processes Cellular processes Chemotaxis and motility flagellar protein export ATPase FliI (TIGR03496; EC 3.6.3.14; HMM-score: 199.1)
    Metabolism Energy metabolism ATP-proton motive force interconversion ATPase, FliI/YscN family (TIGR01026; EC 3.6.3.14; HMM-score: 187.6)
    Cellular processes Cellular processes Chemotaxis and motility flagellar protein export ATPase FliI (TIGR03497; EC 3.6.3.14; HMM-score: 183.5)
    flagellar protein export ATPase FliI (TIGR03498; EC 3.6.3.14; HMM-score: 173.7)
    Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase archaeal, B subunit (TIGR01041; EC 3.6.3.14; HMM-score: 147.9)
    Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F1, beta subunit (TIGR01039; EC 3.6.3.14; HMM-score: 130.1)
    alternate F1F0 ATPase, F1 subunit beta (TIGR03305; EC 3.6.3.-; HMM-score: 121.2)
    Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, B subunit (TIGR01040; EC 3.6.3.14; HMM-score: 119.1)
    Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase archaeal, A subunit (TIGR01043; EC 3.6.3.14; HMM-score: 69.6)
    Metabolism Transport and binding proteins Cations and iron carrying compounds V-type ATPase, A subunit (TIGR01042; EC 3.6.3.14; HMM-score: 51.8)
    Genetic information processing Transcription Transcription factors transcription termination factor Rho (TIGR00767; EC 3.6.4.-; HMM-score: 19.5)
    KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 16.3)
    KaiC domain protein, PAE1156 family (TIGR03881; HMM-score: 15.3)
    KaiC domain protein, Ph0284 family (TIGR03877; HMM-score: 13)
  • TheSEED: see SAUSA300_2060
  • PFAM:
    P-loop_NTPase (CL0023) ATP-synt_ab; ATP synthase alpha/beta family, nucleotide-binding domain (PF00006; HMM-score: 246.9)
    and 7 more
    no clan defined ATP-synt_ab_C; ATP synthase alpha/beta chain, C terminal domain (PF00306; HMM-score: 153.1)
    HAS-barrel (CL0275) ATP-synt_ab_N; ATP synthase alpha/beta family, beta-barrel domain (PF02874; HMM-score: 67.1)
    P-loop_NTPase (CL0023) ATPase; KaiC (PF06745; HMM-score: 27.1)
    HAS-barrel (CL0275) HAS-barrel; HAS barrel domain (PF09378; HMM-score: 21.8)
    P-loop_NTPase (CL0023) RecA; recA bacterial DNA recombination protein (PF00154; HMM-score: 13.1)
    AAA_14; AAA domain (PF13173; HMM-score: 12.9)
    AAA_25; AAA domain (PF13481; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00179
    • TAT(Tat/SPI): 0.00044
    • LIPO(Sec/SPII): 0.000183
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGLNDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEELIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIGRGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGALDYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNVISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYRELESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPVVDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_RS11345&oldid=61727"