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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS10490 [old locus tag: SAUSA300_1916 ]
  • pan locus tag?: SAUPAN005004000
  • symbol: SAUSA300_RS10490
  • pan gene symbol?: aspB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS10490 [old locus tag: SAUSA300_1916 ]
  • symbol: SAUSA300_RS10490
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2080435..2081721
  • length: 1287
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATCCTTTAGCCCAAAGCTTAAATGAACAACTTCAACAGTCAAATGCAACTGCCTTT
    ACAATGTTATCTGACTTAGGTCAAAATATGTTTTATCCAAAAGGCATTTTATCTCAATCT
    GCTGAAGCAAAGAGTACAACATATAATGCAACTATAGGTATGGCGACAAACAAAGACGGA
    AAAATGTTTGCATCATCTTTAGATGCAATGTTTAATGATTTAACTCCAGATGAAATATTC
    CCTTATGCGCCACCACAAGGCATCGAAGAATTACGTGATTTATGGCAACAAAAAATGTTG
    CGTGACAATCCAGAGCTATCAATCGACAACATGTCACTACCAATTGTTACGAATGCATTA
    ACACATGGTTTATCTTTAGTTGGCGATTTATTTGTAAATCAAGGTGACACTATCTTGTTA
    CCAGAGCATAATTGGGGTAATTACAAACTTGTTTTCAATACGAGAAATGGTGCAAACCTT
    CAAACATATCCTATCTTTGATAAAGACGGGCATTATACTACTGATTCACTTGTAGAAGCT
    TTACAATCATACAATAAAGATAAAGTCATTATGATTTTAAATTATCCTAATAATCCGACA
    GGTTACACACCTACGCATAAAGAAGTGACTACCATCGTCGATGCAATTAAAGCATTAGCT
    GATAAAGGTACAAAAGTTATAGCTGTTGTGGATGACGCATACTATGGTTTATTCTATGAA
    GATGTGTATACTCAATCATTATTTACTGCATTATCTAATTTAAATTCAAATGCAATATTA
    CCTGTTCGTTTAGATGGTGCAACAAAAGAATTTTTCGCATGGGGATTCCGTGTTGGGTTT
    ATGACATTTGGAACGTCTGATCAAACAACTAAAGAAGTATTAGAAGCCAAAGTAAAAGGT
    CTTATACGAAGTAACATTTCTAGTGGACCTCTTCCAACACAAAGCGCTGTTAAGCATGTA
    TTAAAAAATAATAAACAATTCGATAAAGAAATCGAACAAAATATTCAAACATTAAAAGAA
    CGCTATGAAGTCACTAAGGAAGTTGTCTATGCTGATCAATATCATTCACATTGGCAAGCC
    TATGACTTTAACTCTGGATACTTTATGGCAATAAAAGTTCATGATGTTGACCCTGAAGAA
    CTTCGTAAACATTTAATTGAAAAATATTCAATTGGTGTTATTGCACTTAATGCAACAGAT
    ATTCGTATTGCGTTTAGCTGTGTAGAAAAAGAAGATATCCCACATGTATTTGATTCAATT
    GCTAAAGCGATTGATGACTTAAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1287

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS10490 [old locus tag: SAUSA300_1916 ]
  • symbol: SAUSA300_RS10490
  • description: hypothetical protein
  • length: 428
  • theoretical pI: 4.88204
  • theoretical MW: 48119
  • GRAVY: -0.316355

⊟Function[edit | edit source]

  • TIGRFAM:
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 64.4)
    Metabolism Energy metabolism Amino acids and amines tyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 62.8)
    LL-diaminopimelate aminotransferase (TIGR03540; EC 2.6.1.83; HMM-score: 56.3)
    and 9 more
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 43.3)
    putative C-S lyase (TIGR04350; EC 4.4.-.-; HMM-score: 35.3)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 32.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin threonine-phosphate decarboxylase (TIGR01140; EC 4.1.1.81; HMM-score: 26.2)
    Cellular processes Cellular processes Biosynthesis of natural products capreomycidine synthase (TIGR03947; HMM-score: 25.8)
    succinyldiaminopimelate transaminase (TIGR03539; EC 2.6.1.17; HMM-score: 24.8)
    LL-diaminopimelate aminotransferase (TIGR03542; EC 2.6.1.83; HMM-score: 19.9)
    succinyldiaminopimelate transaminase (TIGR03537; EC 2.6.1.17; HMM-score: 13.5)
    [FeFe] hydrogenase, group B1/B3 (TIGR04105; EC 1.12.-.-; HMM-score: 10.5)
  • TheSEED: see SAUSA300_1916
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 125.1)
    and 4 more
    Thioredoxin (CL0172) AhpC-TSA; AhpC/TSA family (PF00578; HMM-score: 16)
    no clan defined Spore_II_R; Stage II sporulation protein R (spore_II_R) (PF09551; HMM-score: 14.2)
    4HB_MCP (CL0457) 4HB_MCP_1; Four helix bundle sensory module for signal transduction (PF12729; HMM-score: 13.6)
    no clan defined NEMO; NF-kappa-B essential modulator NEMO (PF11577; HMM-score: 10.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.024746
    • TAT(Tat/SPI): 0.01037
    • LIPO(Sec/SPII): 0.001727
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNPLAQSLNEQLQQSNATAFTMLSDLGQNMFYPKGILSQSAEAKSTTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRDNPELSIDNMSLPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRNGANLQTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKALADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALSNLNSNAILPVRLDGATKEFFAWGFRVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDKEIEQNIQTLKERYEVTKEVVYADQYHSHWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIGVIALNATDIRIAFSCVEKEDIPHVFDSIAKAIDDLR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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