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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS09885 [old locus tag: SAUSA300_1806 ]
  • pan locus tag?: SAUPAN004792000
  • symbol: SAUSA300_RS09885
  • pan gene symbol?: queG
  • synonym:
  • product: epoxyqueuosine reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS09885 [old locus tag: SAUSA300_1806 ]
  • symbol: SAUSA300_RS09885
  • product: epoxyqueuosine reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 1988375..1989517
  • length: 1143
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGGGGGCATACGATTGGATACAAAGCAATTAAAGCAAGATATCATTGACTATGCATAT
    ACAATTGGTATCGACAGTATTGGATTTACTACTGCCGATCCCTTTGATGAATTGAAGCAA
    AAGCTAGAAGCATATCATGCAAATGGTTATGCCTCAGGATTTGAAGAATCTGATATTGCT
    TTACGAACGGAGCCTAAATTATCCTTACCAACAGCAAGGTCAATCATAGCAATTGCAGTT
    GGTTATCCTAACAAACTGAAAGGTGCACCTAAGAGTGTTAGAGGTGATCGCAGAGGCTTA
    TTTGCTAGAGCATCGTGGGGTCAAGATTATCATACAATTATGCGTAAACGATTAGACATG
    TTAGCTGCATTTATTGAATCTAAAGTTCCAGATGTTGAAATCAAATCTATGGTAGATACG
    GGTGTATTATCAGATAGGGCAGTAGCAGAACGTGCAGGTTTAGGATTTGTTGGTCGTAAT
    GGCTTTGTCATCAATCCTAAACTAGGAACATGGACATACCTCGGTGAAATGTTAGTCAGT
    ATACCTTTTGAACCTGATGATCCATTATTAGATAGCTGTGGTGATTGTACAATTTGTGTT
    GATCGTTGTCCAACAAGTGCATTAGTTGGAAATGGCCAATTAAATAGTCAGAAATGCATT
    AGTTTTTTAACGCAAACAAAAGGCTATATGCCTGACCAATATCGTTATAAAATTGGGAAT
    AGATTATATGGTTGCGATACGTGTCAACAAGTTTGTCCGAAAAATAGAGGTATTAATACC
    GAACAAGATGACATCATTTTGGAACCAGAAATTTTAAAGCCAAGATTAGTACCTTTACTA
    CGTATGTCTAATAAAGAATTCAAACAAACATATGGTCACCTTGCAGGTGCTTGGCGTGGT
    AAAAAGCCGATACAACGAAATGCTATTTTAGCATTGGCGCATTTTAATGAAGTGGATGCA
    ATTCCAGAATTGAAAAAAGTTGCAACAACAGATGAAAGACCGTTGATTCGAGCAACTGCT
    TATTGGGCCATTGGTCAAATTCTTGGGGAAGAAGCAAGAGATTTTATCAATGCTAATTAT
    GATCAAGAGGATGCAGAAGTTCAGAATGAAATGATAAAAGGATTAGACACAAGGAGAGAA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS09885 [old locus tag: SAUSA300_1806 ]
  • symbol: SAUSA300_RS09885
  • description: epoxyqueuosine reductase
  • length: 380
  • theoretical pI: 6.52454
  • theoretical MW: 42432.2
  • GRAVY: -0.362632

⊟Function[edit | edit source]

  • reaction:
    EC 1.17.99.6?  ExPASy
    Epoxyqueuosine reductase Queuosine34 in tRNA + acceptor + H2O = epoxyqueuosine34 in tRNA + reduced acceptor
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification epoxyqueuosine reductase (TIGR00276; EC 1.-.-.-; HMM-score: 401.2)
    and 10 more
    reductive dehalogenase (TIGR02486; EC 1.97.1.-; HMM-score: 45.1)
    2-oxoacid:acceptor oxidoreductase, delta subunit, pyruvate/2-ketoisovalerate family (TIGR02179; HMM-score: 19.8)
    Metabolism Energy metabolism Electron transport electron transport complex, RnfABCDGE type, B subunit (TIGR01944; HMM-score: 15.5)
    Metabolism Energy metabolism Electron transport NADH-quinone oxidoreductase, chain I (TIGR01971; HMM-score: 11.6)
    Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00314; EC 1.2.99.2; HMM-score: 10.7)
    Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 10.4)
    pyruvate:ferredoxin (flavodoxin) oxidoreductase (TIGR02176; EC 1.2.7.1; HMM-score: 7.7)
    Genetic information processing Protein fate Protein modification and repair glycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 6.9)
    Metabolism Central intermediary metabolism Nitrogen fixation ferredoxin III, nif-specific (TIGR02936; HMM-score: 5.3)
    methylamine methyltransferase corrinoid protein reductive activase (TIGR04270; HMM-score: 3.7)
  • TheSEED: see SAUSA300_1806
  • PFAM:
    no clan defined DUF1730; Domain of unknown function (DUF1730) (PF08331; HMM-score: 62.9)
    4Fe-4S (CL0344) Fer4_16; 4Fe-4S double cluster binding domain (PF13484; HMM-score: 54.7)
    and 16 more
    TPR (CL0020) HEAT_2; HEAT repeats (PF13646; HMM-score: 32)
    4Fe-4S (CL0344) Fer4_9; 4Fe-4S dicluster domain (PF13187; HMM-score: 26.1)
    Fer4_7; 4Fe-4S dicluster domain (PF12838; HMM-score: 24.7)
    Fer4_10; 4Fe-4S dicluster domain (PF13237; HMM-score: 24.1)
    Fer4; 4Fe-4S binding domain (PF00037; HMM-score: 18.5)
    Fer4_21; 4Fe-4S dicluster domain (PF14697; HMM-score: 17.8)
    Fer4_6; 4Fe-4S binding domain (PF12837; HMM-score: 14.8)
    TPR (CL0020) HEAT_PBS; PBS lyase HEAT-like repeat (PF03130; HMM-score: 13.1)
    HEAT; HEAT repeat (PF02985; HMM-score: 12.8)
    FMN-dep-NRtase (CL0529) Dehalogenase; Reductive dehalogenase subunit (PF13486; HMM-score: 12.6)
    4Fe-4S (CL0344) Fer4_2; 4Fe-4S binding domain (PF12797; HMM-score: 12.4)
    Fer4_8; 4Fe-4S dicluster domain (PF13183; HMM-score: 12.3)
    Fer4_3; 4Fe-4S binding domain (PF12798; HMM-score: 11.6)
    Fer4_17; 4Fe-4S dicluster domain (PF13534; HMM-score: 11.3)
    Fer4_13; 4Fe-4S single cluster domain of Ferredoxin I (PF13370; HMM-score: 10)
    Fer4_18; 4Fe-4S dicluster domain (PF13746; HMM-score: 7.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.78
    • Cytoplasmic Membrane Score: 8.16
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006812
    • TAT(Tat/SPI): 0.000512
    • LIPO(Sec/SPII): 0.00084
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGGIRLDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANGYASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWGQDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVINPKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFLTQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLRMSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPLIRATAYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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