PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS07355 [old locus tag: SAUSA300_1349 ]
• pan locus tag^?: SAUPAN003918000
• symbol: SAUSA300_RS07355
• pan gene symbol^?: bshA
• synonym:
• product: N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS07355 [old locus tag: SAUSA300_1349 ]
• symbol: SAUSA300_RS07355
• product: N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA
• replicon: chromosome
• strand: -
• coordinates: 1516184..1517326
• length: 1143
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1516184..1517326) NCBI
• BioCyc: SAUSA300_RS07355 BioCyc
• MicrobesOnline: see SAUSA300_1349

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  481
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  661
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  841
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  1021
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  1141
  
  ATGAAGATAGGTATAACATGTTACCCGTCCATGGGTGGTTCTGGAATTATTGCCACAGAA
  TTAGGAATTAAATTAGCAGAGCGAGGTCACGAAGTACATTTTATCACTTCAAATATCCCG
  TTTAGAATAAGAAAACCATTACCGAATATGATTTTTCATCAAGTTGAAGTGAATCAATAT
  GCAGTATTTCAGTATCCACCGTATGATATTACGTTAAGTACTAAAATCGCTGAAGTTATT
  AAAGAATATGATCTTGATTTGTTGCATATGCACTATGCTGTACCTCATGCAATTTGTGGG
  ATTTTAGCTCGTGAGATGTCAGGTAAAGATATAAAAATTATGACAACGCTACACGGTACT
  GATATTACTGTTTTGGGCTATGATCATTCACTCCAAGGTGCAATTAAATTTGGCATTGAA
  AAAAGTGATATTGTGACAAGTGTGAGTAAATCTTTAGCACAAGAAACACATGAAATTATT
  GAAACTAACAAAGAAATTATACCGATTTATAATTTTGTACGTGAAAATGAGTTTCCAACT
  AAACATAACACAGCATTAAAATCACAATTTGGTATAGCACCAGATGAAAAAGTGTTAATA
  CATGTATCGAATTTTAGACAAGTAAAACGCATAGATACTATTATAGAAACATTTGCAAAA
  GTACGTGAAAAGATACCTAGTAAATTAATTTTACTTGGAGATGGTCCTGAATTAGTGCCA
  ATGCGTCAATTGACGAAAGAGTTAAACGTAGAAGAGGATGTTTTATTTTTAGGGAAACAA
  GATTGCGTAAGTGAATTTTACCAATTATCTGACTTAGTATTATTATTAAGTGAAAAAGAA
  AGCTTTGGACTAACTTTACTTGAGGCTATGAAAACAGGCGTCGTTCCAATTGGTTCAAAT
  GCCGGTGGAATTAAAGAGGTTATTAAACATGGTGAAACTGGATTTGTCGTAGATGTGGGT
  GATTGTGATTCAGCGAGTGACTATGCTATCCGATTACTTGAAGATAAGGTGTTATACAAC
  AAACTTCAGAAAAACATGCTAGCAGATATTGCTGAACGTTTTGGATCAGAACTTATTACA
  GATCAATATGAGTATTATTATCAAAAAATGCTAAATGAGCATAACAAAAGTAAAGGCGAG
  TAA
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  1143
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS07355 [old locus tag: SAUSA300_1349 ]
• symbol: SAUSA300_RS07355
• description: N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA
• length: 380
• theoretical pI: 5.75583
• theoretical MW: 42795.1
• GRAVY: -0.127632

⊟Function

• reaction:
  EC 2.4.1.-?  ExPASy
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 553.2)
  and 13 more
  sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 93.2)
  glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 78.9)
  PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 75.7)
  D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 61.4)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 61.3)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 54.8)
  Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 41.9)
  Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 38.9)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 30.1)
  colanic acid biosynthesis glycosyltransferase WcaI (TIGR04007; EC 2.4.-.-; HMM-score: 29.7)
  sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 26.9)
  sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 25.3)
  colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 24)
• TheSEED: see SAUSA300_1349
• PFAM:
  GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 128.6)
  and 7 more
  Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 84.6)
  Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 70.7)
  Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 47)
  Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 29.4)
  Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 24.1)
  Glyco_transf_5; Starch synthase catalytic domain (PF08323; HMM-score: 22.2)
  NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 14.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.018066
  • TAT(Tat/SPI): 0.000537
  • LIPO(Sec/SPII): 0.004663
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446612681 NCBI
• RefSeq: WP_000690027 NCBI
• UniProt: see SAUSA300_1349

⊟Protein sequence

• MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIRKPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGILAREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEIIETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETFAKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLLSEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLEDKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications