PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS06640 [old locus tag: SAUSA300_1225 ]
• pan locus tag^?: SAUPAN003698000
• symbol: SAUSA300_RS06640
• pan gene symbol^?: thrD
• synonym:
• product: aspartate kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS06640 [old locus tag: SAUSA300_1225 ]
• symbol: SAUSA300_RS06640
• product: aspartate kinase
• replicon: chromosome
• strand: -
• coordinates: 1341817..1343199
• length: 1383
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1341817..1343199) NCBI
• BioCyc: SAUSA300_RS06640 BioCyc
• MicrobesOnline: see SAUSA300_1225

⊟Phenotype

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⊟DNA sequence

• 1
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  481
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  1021
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  1141
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  1321
  1381
  
  ATGAAAGTTTCCAAATTCGGTGGTAGCTCCGTCTCCAATGCTACTCAAATTAAAAAGGTT
  TTAAATATTGTTAATTCTGATCCAGAGCGAAAGATTGTTATCGTTTCTGCTCCAGGTAAA
  AGACATGATAATGATATTAAAACAACTGATTTGTTAATCAGATTATATGAAAAGGTCATT
  AATCATCTTGATTATCATGATAAAAAAAGAGAAATTATTCAGCGTTATGATGATATTGTA
  AAAGAATTGCAAATGGATGAAAGTATTTTACGGACGATAGATGTGACTTTGGAACATTAT
  ATTAATCAATTAAAAAATGAACCAAAGAGACTATTAGATGCATTACTTTCTTGTGGTGAA
  GATTTTAATGCGCAATTGATAGCTTTATATAATAATAGTCAAGGTATACCAACAAAATAT
  ATATCACCGAAGGAAGCGGGTATTTTTGTTACTGACGTCCCACAAGAAGCACAAATATTA
  GATAGTGCTTACTCTGAAATTAAAAAATTAAGAGATTATGATGAAAAACTAATTATCCCA
  GGATTTTTTGGCGTATCTCATGAAGGATATATAGTTACATTTCCACGTGGCGGATCAGAT
  ATAACTGGCGCCATCATATCAAGTGGTGTTAGAGCCACAATTTATGAAAATTTCACCGAT
  GTGTCTGGTATTTATAAAGCTAATCCAAATATCATCAAAGATCCTGAATTAATCGAAGAA
  ATCACTTATCGAGAAATGCGTGAATTATCTTATGCTGGTTTTAGTGTTTTCCATGATGAA
  GCCTTACAACCACTTTATAAAGATCGCATCCCTGTGGTGATTAAAAATACGAACCGCCCT
  CAAGATAAGGGTACATTTATAGTGCATGATAGAGAAATAAATGCGAAAAATGTGATTAGC
  GGTATAAGTTGTGATAAAGATTTTACCGTTATTAATATCAAAAAGTATTTAATGAATAGA
  CAAGTCGGCTTTACTAGAAAGATATTAGGTGTCTTAGAGGATAATAATATTTCATTTGAT
  CATATGCCTTCAGGTATTGATACTATCAGTATCGTTATGCGTTCAAAACAAATTCAAAAT
  AAAGAAACAAAAGTATTAAATGAAATTCGTCAAAAATGTGATGTTGATGAGCTAAGTATT
  GAGCATGATTTAGCAATATTAATGATTGTCGGCGAAGGTATGAATAAAGTCATCGGTACT
  GCAAATAAAATTACACATGCCTTAGCTGAATCAAAAATTAACTTAAAAATGATTAACCAA
  GGTGCTTCGGAAATCTCGATGATGTTTGGAATTCATGTAGATGATGCTGAAAAAGCTGTA
  TTATCTACATATGAGTTTTGCTATCACGGGGTTGCATTAAAAAACTTGTGTGATAAAAAC
  TAA
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  1383
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS06640 [old locus tag: SAUSA300_1225 ]
• symbol: SAUSA300_RS06640
• description: aspartate kinase
• length: 460
• theoretical pI: 6.38678
• theoretical MW: 52003.3
• GRAVY: -0.28087

⊟Function

• reaction:
  EC 2.7.2.4?  ExPASy
  Aspartate kinase ATP + L-aspartate = ADP + 4-phospho-L-aspartate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 285.2)
  Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 254.9)
  and 2 more
  Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 91.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 20.5)
• TheSEED: see SAUSA300_1225
• PFAM:
  no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 142.4)
  and 2 more
  ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 36.2)
  ACT_7; ACT domain (PF13840; HMM-score: 31.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003722
  • TAT(Tat/SPI): 0.00028
  • LIPO(Sec/SPII): 0.001227
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446791389 NCBI
• RefSeq: WP_000868645 NCBI
• UniProt: see SAUSA300_1225

⊟Protein sequence

• MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDNDIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYINQLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQILDSAYSEIKKLRDYDEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENFTDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKNTNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLEDNNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGEGMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHGVALKNLCDKN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SAUSA300_1225

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications