PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS04570 [old locus tag: SAUSA300_0846 ]
• pan locus tag^?: SAUPAN003043000
• symbol: SAUSA300_RS04570
• pan gene symbol^?: —
• synonym:
• product: sodium:proton antiporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS04570 [old locus tag: SAUSA300_0846 ]
• symbol: SAUSA300_RS04570
• product: sodium:proton antiporter
• replicon: chromosome
• strand: +
• coordinates: 925162..926478
• length: 1317
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (925162..926478) NCBI
• BioCyc: SAUSA300_RS04570 BioCyc
• MicrobesOnline: see SAUSA300_0846

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGATAAATGCAGTAGTAATAGCAGTAATTTTAATGATTGTGCTATGTTTATGTCGATTA
  AACGTAGTTATAAGCTTATTTATCAGTGCGCTAGTTGGTGGCTTAATTTCAGGCATGAGC
  ATTGAAAAAGTTATAAATGTATTTGGGAAAAATATAGTCGATGGTGCTGAGGTAGCATTA
  AGCTATGCTTTATTAGGTGGATTTGCAGCATTAATTTCATACAGTGGTATCACAGACTAT
  TTAGTAGGAAAAATTATAAATGCAATTCACGCTGAAAATAGTCGATGGTCAAGAGTTAAA
  GTCAAAGTGACAATAATCATTGCATTATTAGCTATGAGTATCATGAGTCAAAACTTAATT
  CCTGTACATATTGCATTCATTCCAATTGTCATCCCACCATTGTTAAGTCTGTTTAATGAC
  TTAAAAATAGATAGACGTTTAATCGGTTTGATTATCGGTTTTGGTTTATGTTTCCCGTAT
  GTGTTATTACCATATGGATTCGGTCAAATTTTCCAGCAAATTATTCAAAGTGGCTTTGCA
  AAGGCAAATCACCCAATTGAGTTTAATATGATTTGGAAAGCAATGCTTATTCCTTCAATG
  GGGTATATTGTTGGCTTACTTATCGGTTTATATGTATATCGTAAACCACGTGAATATGAA
  ACACGTAAAATTTCAGATAGTGACAATGTTACAGAGTTAAAACCATATATCTTAATAGTA
  ACAATTGTAGCAATACTAGCTACATTTTTAGTACAAACATTTACAGATTCAATGATTTTT
  GGTGCACTGGCAGGGGTACTCGTATTCTTTATTTCACGTGCATATAATTGGTATGAATTA
  GATGCTAAGTTTGTTGAAGGTATTAAAATTATGGCTTATATTGGTGTAGTTATTTTAACA
  GCAAATGGATTTGCTGGTGTAATGAATGCTACTGGTGATATAGATGAATTAGTTAAAACT
  TTAACAAGTATTACTGGTGATAATAAATTATTTAGCATTATCATGATGTATGTGATAGGT
  TTAATTGTCACTTTAGGTATTGGATCATCATTTGCAACAATTCCTATTATCGCATCATTA
  TTCATTCCTTTTGGAGCGTCAATTGGACTAGATACAATGGCATTAATCGCATTGATTGGA
  ACAGCGAGTGCATTAGGTGACTCAGGTTCGCCTGCAAGTGATTCAACATTAGGACCAACT
  GCGGGATTAAATGTTGATGGCCAACATGATCATATACGTGATACATGTGTACCAAACTTC
  TTGTTTTATAATATTCCTTTAATGATTTTCGGTACTATTGCTGCTATGGTACTATAA
  60
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  1020
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  1140
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  1260
  1317
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS04570 [old locus tag: SAUSA300_0846 ]
• symbol: SAUSA300_RS04570
• description: sodium:proton antiporter
• length: 438
• theoretical pI: 6.6689
• theoretical MW: 47279.2
• GRAVY: 0.940868

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids TRAP transporter, DctM subunit (TIGR00786; HMM-score: 23.7)
  and 1 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids transporter, gluconate:H+ symporter (GntP) family (TIGR00791; HMM-score: 11.4)
• TheSEED: see SAUSA300_0846
• PFAM:
  IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 245.7)
  and 6 more
  Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 113)
  DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 39.7)
  DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 24.9)
  DUF969; Protein of unknown function (DUF969) (PF06149; HMM-score: 15.7)
  no clan defined AzlD; Branched-chain amino acid transport protein (AzlD) (PF05437; HMM-score: 13.9)
  IT (CL0182) GntP_permease; GntP family permease (PF02447; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 11
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9998
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0002
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.05255
  • TAT(Tat/SPI): 0.000716
  • LIPO(Sec/SPII): 0.068386
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 446528978 NCBI
• RefSeq: WP_000606324 NCBI
• UniProt: see SAUSA300_0846

⊟Protein sequence

• MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKVINVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVKVTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPYVLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPREYETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYNWYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSIIMMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPASDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: HisR* see SAUSA300_0846

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications