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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS03845 [old locus tag: SAUSA300_0716 ]
- pan locus tag?: SAUPAN002630000
- symbol: SAUSA300_RS03845
- pan gene symbol?: nrdE
- synonym:
- product: ribonucleotide-diphosphate reductase subunit alpha
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS03845 [old locus tag: SAUSA300_0716 ]
- symbol: SAUSA300_RS03845
- product: ribonucleotide-diphosphate reductase subunit alpha
- replicon: chromosome
- strand: +
- coordinates: 794134..796239
- length: 2106
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (794134..796239) NCBI
- BioCyc: SAUSA300_RS03845 BioCyc
- MicrobesOnline: see SAUSA300_0716
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2101ATGAAAACCATGGAAGAGAAAAAGTACAATCATATTGAATTAAATAATGAGGTCACTAAA
CGAAGAGAAGATGGATTCTTTAGTTTAGAAAAAGACCAAGAAGCTTTAGTAGCTTATTTA
GAAGAAGTAAAAGACAAAACAATCTTCTTCGACACTGAAATCGAGCGTTTACGTTATTTA
GTAGACAACGATTTTTATTTCAATGTGTTTGATATTTATAGTGAAGCGGATCTAATTGAA
ATCACTGATTATGCAAAATCAATCCCGTTTAATTTTGCAAGTTATATGTCAGCTAGTAAA
TTTTTCAAAGATTACGCTTTGAAAACAAATGATAAAAGTCAATACTTAGAAGACTATAAT
CAACACGTTGCCATTGTTGCTTTATACCTAGCAAATGGTAATAAAGCACAAGCTAAACAA
TTTATTTCTGCTATGGTTGAACAAAGATATCAACCAGCGACACCAACATTTTTAAACGCA
GGCCGTGCGCGTCGTGGTGAGCTAGTGTCATGTTTCTTATTAGAAGTGGATGACAGCTTA
AATTCAATTAACTTTATTGATTCAACTGCAAAACAATTAAGTAAAATTGGGGGCGGCGTT
GCAATTAACTTATCTAAATTGCGTGCACGTGGTGAAGCAATTAAAGGAATTAAAGGCGTA
GCGAAAGGCGTTTTACCTATTGCTAAGTCACTTGAAGGTGGCTTTAGCTATGCAGATCAA
CTTGGTCAACGCCCTGGTGCTGGTGCTGTGTACTTAAATATCTTCCATTATGATGTAGAA
GAATTTTTAGATACTAAAAAAGTAAATGCGGATGAAGATTTACGTTTATCTACAATATCA
ACTGGTTTAATTGTTCCATCTAAATTCTTCGATTTAGCTAAAGAAGGTAAGGACTTTTAT
ATGTTTGCACCTCATACAGTTAAAGAAGAATATGGTGTGACATTAGACGATATCGATTTA
GAAAAATATTATGATGACATGGTTGCAAACCCAAATGTTGAGAAAAAGAAAAAGAATGCG
CGTGAAATGTTGAATTTAATTGCGCAAACACAATTACAATCAGGTTATCCATATTTAATG
TTTAAAGATAATGCTAACAGAGTGCATCCGAATTCAAACATTGGACAAATTAAAATGAGT
AACTTATGTACGGAAATTTTCCAACTACAAGAAACTTCAATTATTAATGACTATGGTATT
GAAGACGAAATTAAACGTGATATTTCTTGTAACTTGGGCTCATTAAATATTGTTAATGTA
ATGGAAAGCGGAAAATTCAGAGATTCAGTTCACTCTGGTATGGACGCATTAACTGTTGTG
AGTGATGTAGCAAATATTCAAAATGCACCAGGAGTTAGAAAAGCTAACAGTGAATTACAT
TCAGTTGGTCTTGGTGTGATGAATTTACACGGTTACCTAGCAAAAAATAAAATTGGTTAT
GAGTCAGAAGAAGCAAAAGATTTTGCAAATATCTTCTTTATGATGATGAATTTCTACTCA
ATCGAACGTTCAATGGAAATCGCTAAAGAGCGTGGTATCAAATATCAAGACTTTGAAAAG
TCTGATTATGCTAATGGCAAATATTTCGAGTTCTATACAACTCAAGAATTTGAACCTCAA
TTCGAAAAAGTACGTGAATTATTCGATGGTATGGCTATTCCTACTTCTGAGGATTGGAAG
AAACTACAACAAGATGTTGAACAATATGGTTTATATCATGCATATAGATTAGCAATTGCT
CCAACACAAAGTATTTCTTATGTTCAAAATGCAACAAGTTCTGTAATGCCAATCGTTGAC
CAAATTGAACGTCGTACTTATGGTAATGCGGAAACATTTTACCCTATGCCATTCTTATCA
CCACAAACAATGTGGTACTACAAATCAGCATTCAATACTGATCAGATGAAATTAATCGAT
TTAATTGCGACAATTCAAACGCATATTGACCAAGGTATCTCAACGATCCTTTATGTTAAT
TCTGAAATTTCTACACGTGAGTTAGCAAGATTATATGTATATGCGCACTATAAAGGATTA
AAATCACTTTACTATACTAGAAATAAATTATTAAGTGTAGAAGAATGTACAAGTTGTTCT
ATCTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS03845 [old locus tag: SAUSA300_0716 ]
- symbol: SAUSA300_RS03845
- description: ribonucleotide-diphosphate reductase subunit alpha
- length: 701
- theoretical pI: 4.99873
- theoretical MW: 80125.2
- GRAVY: -0.373894
⊟Function[edit | edit source]
- reaction: EC 1.17.4.1? ExPASyRibonucleoside-diphosphate reductase 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin
- TIGRFAM: ribonucleoside-diphosphate reductase, class 1b, alpha subunit (TIGR04170; EC 1.17.4.1; HMM-score: 1139.7)and 3 morePurines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, alpha subunit (TIGR02506; EC 1.17.4.1; HMM-score: 544.3)Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent (TIGR02504; EC 1.17.4.1; HMM-score: 229.3)Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, alpha chain (TIGR02510; EC 1.17.4.1; HMM-score: 131.3)
- TheSEED: see SAUSA300_0716
- PFAM: PFL-like (CL0339) Ribonuc_red_lgC; Ribonucleotide reductase, barrel domain (PF02867; HMM-score: 462.2)and 4 moreno clan defined RNR_N; Ribonucleotide reductase N-terminal (PF08343; HMM-score: 71.7)Ribonuc_red_lgN; Ribonucleotide reductase, all-alpha domain (PF00317; HMM-score: 51.9)Caud_tail_N; Caudoviral major tail protein N-terminus (PF16838; HMM-score: 14.9)Alk_phosphatase (CL0088) DUF4976; Domain of unknown function (DUF4976) (PF16347; HMM-score: 10.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002481
- TAT(Tat/SPI): 0.000262
- LIPO(Sec/SPII): 0.000487
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446778249 NCBI
- RefSeq: WP_000855505 NCBI
- UniProt: see SAUSA300_0716
⊟Protein sequence[edit | edit source]
- MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFFKDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS01155 formate acetyltransferase [1] (data from MRSA252) SAUSA300_RS02845 elongation factor G [1] (data from MRSA252) SAUSA300_RS02850 elongation factor Tu [1] (data from MRSA252) SAUSA300_RS04560 NADH dehydrogenase [1] (data from MRSA252) SAUSA300_RS06135 50S ribosomal protein L19 [1] (data from MRSA252) SAUSA300_RS09015 universal stress protein [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12105 30S ribosomal protein S17 [1] (data from MRSA252) SAUSA300_RS12140 50S ribosomal protein L23 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: NrdR see SAUSA300_0716
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)