PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS01705 [old locus tag: SAUSA300_0320 ]
• pan locus tag^?: SAUPAN001245000
• symbol: SAUSA300_RS01705
• pan gene symbol^?: gehB
• synonym:
• product: lipase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS01705 [old locus tag: SAUSA300_0320 ]
• symbol: SAUSA300_RS01705
• product: lipase
• replicon: chromosome
• strand: +
• coordinates: 372415..374487
• length: 2073
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (372415..374487) NCBI
• BioCyc: SAUSA300_RS01705 BioCyc
• MicrobesOnline: see SAUSA300_0320

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTTAAGAGGACAAGAAGAAAGAAAGTATAGTATTAGAAAGTATTCAATAGGCGTGGTG
  TCAGTGTTAGCGGCTACAATGTTTGTTGTGTCATCACATGAAGCACAAGCCTCGGAAAAA
  ACATCAACTAATGCAGCGGCACAAAAAGAAACACTAAATCAACCGGGAGAACAAGGGAAT
  GCGATAACGTCACATCAAATGCAGTCAGGAAAGCAATTAGACGATATGCATAAAGAGAAT
  GGTAAAAGTGGAACAGTGACAGAAGGTAAAGATACGCTTCAATCATCGAAGCATCAATCA
  ACACAAAATAGTAAAACAATCAGAATGCAAAATGATAATCAAGTAAAGCAAGATTCTGAA
  CGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTCAAAAT
  GATCAGGTTCAAAATACCCATCATGCTGAACGTAATGGATCACAATCGACAACGTCACAA
  TCGAATGATGTTGATAAATCACAACCATCCATTCCGGCACAAAAGGTAATACCCAATCAT
  GATAAAGCAGCACCAACTTCAACTACACCCCCGTCTAATGATAAAACTGCACCTAAATCA
  ACAAAAGCACAAGATGCAACCACGGACAAACATCCAAATCAACAAGATACACATCAACCT
  GCGCATCAAATCATAGATGCAAAGCAAGATGATACTGTTCGCCAAAGTGAACAGAAACCA
  CAAGTTGGCGATTTAAGTAAACATATCGATGGTCAAAATTCCCCAGAGAAACCGACAGAT
  AAAAATACTGATAATAAACAACTAATCAAAGATGCGCTTCAAGCGCCTAAAACACGTTCG
  ACTACAAATGCAGCAGCAGATGCTAAAAAGGTTCGACCACTTAAAGCGAATCAAGTACAA
  CCACTTAACAAATATCCAGTTGTTTTTGTACATGGATTTTTAGGATTAGTAGGCGATAAT
  GCACCTGCTTTATATCCAAATTATTGGGGTGGAAATAAATTTAAAGTTATCGAAGAATTG
  AGAAAGCAAGGCTATAATGTACATCAAGCAAGTGTAAGTGCATTTGGTAGTAACTATGAT
  CGCGCTGTAGAACTTTATTATTACATTAAAGGTGGTCGCGTAGATTATGGCGCAGCACAT
  GCAGCTAAATACGGACATGAGCGCTATGGTAAGACTTATAAAGGAATCATGCCTAATTGG
  GAACCTGGTAAAAAGGTACATCTTGTAGGGCATAGTATGGGTGGTCAAACAATTCGTTTA
  ATGGAAGAGTTTTTAAGAAATGGTAACAAAGAAGAAATTGCCTATCATAAAGCGCATGGT
  GGAGAAATATCACCATTATTCACTGGTGGTCATAACAATATGGTTGCATCAATCACAACA
  TTAGCAACACCACATAATGGTTCACAAGCAGCTGATAAGTTTGGAAATACAGAAGCTGTT
  AGAAAAATCATGTTCGCTTTAAATCGATTTATGGGTAACAAGTATTCGAATATCGATTTA
  GGATTAACGCAATGGGGCTTTAAACAATTACCAAATGAGAGTTACATTGACTATATAAAA
  CGCGTTAGTAAAAGCAAAATTTGGACATCAGACGACAATGCTGCCTATGATTTAACGTTA
  GATGGCTCTGCAAAATTGAACAACATGACAAGTATGAATCCTAATATTACGTATACGACT
  TATACAGGTGTATCATCTCATACTGGTCCATTAGGTTATGAAAATCCTGATTTAGGTACA
  TTTTTCTTAATGGCTACAACGAGTAGAATTATTGGTCATGATGCAAGAGAAGAATGGCGT
  AAAAATGATGGTGTCGTACCAGTGATTTCGTCATTACATCCGTCCAATCAACCATTTGTT
  AATGTTACGAATGATGAACCTGCCACACGCAGAGGTATCTGGCAAGTTAAACCAATCATA
  CAAGGATGGGATCATGTCGATTTTATCGGTGTGGACTTCCTGGATTTCAAACGTAAAGGT
  GCAGAACTTGCCAACTTCTATACAGGTATTATAAATGACTTGTTGCGTGTTGAAGCGACT
  GAAAGTAAAGGAACACAATTGAAAGCAAGTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS01705 [old locus tag: SAUSA300_0320 ]
• symbol: SAUSA300_RS01705
• description: lipase
• length: 690
• theoretical pI: 9.55352
• theoretical MW: 76417.2
• GRAVY: -0.869565

⊟Function

• reaction:
  EC 3.1.1.3?  ExPASy
  Triacylglycerol lipase Triacylglycerol + H₂O = diacylglycerol + a carboxylate
• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 48.5)
• TheSEED: see SAUSA300_0320
• PFAM:
  AB_hydrolase (CL0028) Lip_C; Lipase-like, C-terminal domain (PF24708; HMM-score: 760.2)
  and 5 more
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 40.3)
  AB_hydrolase (CL0028) Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 21.5)
  Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 20.6)
  LCAT; Lecithin:cholesterol acyltransferase (PF02450; HMM-score: 15.4)
  PGAP1; PGAP1-like alpha/beta domain (PF07819; HMM-score: 14.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.02
  • Extracellular Score: 9.98
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0002
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0.0007
  • Extracellular Score: 0.9989
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 37 aa
  • SP(Sec/SPI): 0.973163
  • TAT(Tat/SPI): 0.017617
  • LIPO(Sec/SPII): 0.002754
  • Cleavage Site: CS pos: 37-38. AQA-SE. Pr: 0.9311
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446866576 NCBI
• RefSeq: WP_000943832 NCBI
• UniProt: see SAUSA300_0320

⊟Protein sequence

• MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTSTNAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQNSKTIRMQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQSNDVDKSQPSIPAQKVIPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTHQPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAPKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKFKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGGHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFKQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSSHTGPLGYENPDLGTFFLMATTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTNDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATESKGTQLKAS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications