PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0309 [new locus tag: SA_RS01780 ]
• pan locus tag^?: SAUPAN001245000
• symbol: geh
• pan gene symbol^?: gehB
• synonym:
• product: glycerol ester hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0309 [new locus tag: SA_RS01780 ]
• symbol: geh
• product: glycerol ester hydrolase
• replicon: chromosome
• strand: +
• coordinates: 365458..367533
• length: 2076
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123088 NCBI
• RefSeq: NP_373555 NCBI
• BioCyc: see SA_RS01780
• MicrobesOnline: 102581 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTTAAGAGGACAAGAAGAAAGAAAGTATAGTATTAGAAAGTATTCAATAGGCGTGGTG
  TCAGTGTTAGCGGCTACAATGTTTGTTGTGTCATCACATGAAGCACAAGCCTCGGAAAAA
  ACACCAACGTCCAATGCAGCGGCACAAAAAGAAACACTAAATCAACCGGGAGAACAAGGG
  AACGCGATAACGTCACATCAAATGCAGTCAGGAAAGCAATTAGACGATATGCATAAAGAG
  AATGGTAAAAGTGGAACAGTGACAGAAGGTAAAGACACGCTTCAATCATCGAAGCATCAA
  TCAACACAAAATAGTAAAACAATCAGAACGCAAAATGATAATCAAGTAAAGCAAGATTCT
  GAACGACAAGGTTCTAAACAGTCACACCAAAATAATGCGACTAATAATACTGAACGTCAA
  AATGATCAGGTTCAAAATACCCATCATGCTGAACGTAATGGATCACAATCGACAACGTCA
  CAATCGAATGATGTTGATAAATCACAACCATCCATTCCGGCACAAAAGGTATTACCCAAT
  CATGATAAAGCAGCACCAACTTCAACTACACCCCCGTCTAATGATAAAACTGCACCTAAA
  TCAACAAAAGCACAAGATGCAACCACGGACAAACATCCAAATCAACAAGATACACATCAA
  CCCGCGCATCAAATCATAGATGCAAAGCAAGATGATACTGTTCGCCAAAGTGAACAGAAA
  CCACAAGTTGGCGATTTAAGTAAACATATCGATGGTCAAAATTCCCCAGAGAAACCGACA
  GATAAAAATACTGATAATAAACAACTAATCAAAGATGCGCTTCAAGCGCCTAAAACACGT
  TCGACTACAAATGCAGCAGCAGATGCTAAAAAGGTTCGACCACTTAAAGCGAATCAAGTA
  CAACCACTTAACAAATATCCAGTTGTTTTTGTACATGGATTTTTAGGATTAGTAGGCGAT
  AATGCACCTGCTTTATATCCAAATTATTGGGGTGGAAATAAATTTAAAGTTATCGAGGAA
  TTGAGAAAGCAAGGCTATAATGTACATCAAGCAAGTGTAAGTGCATTTGGTAGTAACTAT
  GATCGCGCTGTAGAACTTTATTATTACATTAAAGGTGGTCGCGTAGATTATGGCGCAGCA
  CATGCAGCTAAATACGGACATGAGCGCTATGGTAAGACTTATAAAGGAATCATGCCTAAT
  TGGGAACCTGGTAAAAAGGTACATCTTGTAGGGCATAGTATGGGTGGTCAAACAATTCGT
  TTAATGGAAGAGTTTTTAAGAAATGGTAACAAAGAAGAAATTGCCTATCATAAAGCGCAT
  GGTGGAGAAATATCACCATTATTCACTGGTGGTCATAACAATATGGTTGCATCAATCACA
  ACATTAGCAACACCACATAATGGTTCACAAGCAGCTGATAAGTTTGGAAATACAGAAGCT
  GTTAGAAAAATCATGTTCGCTTTAAATCGATTTATGGGTAACAAGTATTCGAATATCGAT
  TTAGGATTAACGCAATGGGGCTTTAAACAATTACCAAATGAGAGTTACATTGACTATATA
  AAACGCGTTAGTAAAAGCAAAATTTGGACATCAGACGATAATGCTGCCTATGATTTAACG
  TTAGATGGCTCTGCAAAATTGAACAACATGACAAGTATGAATCCTAATATTACGTATACG
  ACTTATACAGGTGTGTCTTCACATACTGGTCCATTAGGTTATGAAAATCCTGATTTAGGT
  ACATTTTTCTTAATGGATACAACGAGTAGAATTATTGGTCATGATGCAAGAGAAGAATGG
  CGTAAAAATGATGGTGTCGTACCAGTGATTTCGTCGTTACATCCATCCAATCAACCATTT
  ATTAATGTTACGAATGATGAACCTGCCACACGCAGAGGTATCTGGCAAGTTAAACCAATC
  ATACAAGGATGGGATCATGTCGATTTTATCGGTGTGGACTTCCTGGATTTCAAACGTAAA
  GGTGCAGAACTTGCCAACTTCTATACAGGTATTATAAATGACTTGTTGCGTGTGGAAGCG
  ACTGAAAGTAAAGGAACACAATTGAAAGCAAGTTAA
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⊟Protein

⊟General

• locus tag: SA0309 [new locus tag: SA_RS01780 ]
• symbol: Geh
• description: glycerol ester hydrolase
• length: 691
• theoretical pI: 9.48776
• theoretical MW: 76542.3
• GRAVY: -0.882634

⊟Function

• reaction:
  EC 3.1.1.3?  ExPASy
  Triacylglycerol lipase Triacylglycerol + H₂O = diacylglycerol + a carboxylate
• TIGRFAM:
  gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 48.5)
• TheSEED  :
  • Triacylglycerol lipase (EC 3.1.1.3)
  Fatty Acids, Lipids, and Isoprenoids Triacylglycerols Triacylglycerol metabolism  Triacylglycerol lipase (EC 3.1.1.3)
• PFAM:
  AB_hydrolase (CL0028) Lip_C; Lipase-like, C-terminal domain (PF24708; HMM-score: 757.2)
  and 5 more
  no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 40.3)
  AB_hydrolase (CL0028) Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 21.1)
  Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 20.6)
  LCAT; Lecithin:cholesterol acyltransferase (PF02450; HMM-score: 15.4)
  PGAP1; PGAP1-like alpha/beta domain (PF07819; HMM-score: 14.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.02
  • Extracellular Score: 9.98
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0.0003
  • Cytoplasmic Membrane Score: 0.0002
  • Cell wall & surface Score: 0.0013
  • Extracellular Score: 0.9982
• LocateP: N-terminally anchored (with CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 3
  • Predicted Cleavage Site: HEAQASEK
• SignalP: Signal peptide SP(Sec/SPI) length 37 aa
  • SP(Sec/SPI): 0.972153
  • TAT(Tat/SPI): 0.017636
  • LIPO(Sec/SPII): 0.003282
  • Cleavage Site: CS pos: 37-38. AQA-SE. Pr: 0.9378
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926022 NCBI
• RefSeq: NP_373555 NCBI
• UniProt: Q7A7P2 UniProt

⊟Protein sequence

• MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTSNAAAQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQSTQNSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQSNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTHQPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAPKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKFKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGGHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFKQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSSHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFINVTNDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATESKGTQLKAS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications