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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS00995 [old locus tag: SAUSA300_0190 ]
- pan locus tag?: SAUPAN001030000
- symbol: SAUSA300_RS00995
- pan gene symbol?: —
- synonym:
- product: indolepyruvate decarboxylase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS00995 [old locus tag: SAUSA300_0190 ]
- symbol: SAUSA300_RS00995
- product: indolepyruvate decarboxylase
- replicon: chromosome
- strand: -
- coordinates: 219419..221059
- length: 1641
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (219419..221059) NCBI
- BioCyc: SAUSA300_RS00995 BioCyc
- MicrobesOnline: see SAUSA300_0190
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1621ATGAAACAACGCATTGGAGCTTACTTAATTGACGCTATTCATCGAGCAGGCGTCGATAAA
ATTTTTGGTGTTCCTGGTGATTTTAATCTCGCTTTTCTAGACGATATTATCAGCAATCCC
AATGTAGATTGGGTAGGAAATACAAATGAATTAAACGCAAGTTACGCAGCGGACGGTTAT
GCCCGTCTTAATGGACTCGCTGCATTAGTTACTACATTTGGTGTTGGCGAATTAAGTGCC
GTCAACGGTATCGCAGGTTCATATGCTGAACGCATACCTGTCATTGCGATTACAGGTGCG
CCGACACGTGCTGTTGAACAAGGCGGTAAATATGTACATCACTCACTTGGTGAAGGTACA
TTTGACGACTATCGAAAAATGTTTGCACATATAACCGTTGCACAAGGTTATATCACACCT
GAAAATGCAACAACCGAAATACCACGTTTAATTAATACAGCAATCGCCGAAAGACGCCCA
GTTCATTTACATTTACCAATCGATGTCGCAATCTCTGAAATTGAGATACCGACACCATTT
GAAGTGACGGCAACTAAATATACGGATGCATCAACATATATAGAGTTATTAGCAACTAAA
CTGCATCAAGCGAAGCAGCCTATCATCATTACTGGACATGAAATTAACAGTTTTCACCTC
CATCAAGAATTAGAAGATTTTGTAAATCAAACACAGATACCAGTAGCACAACTTTCATTA
GGAAAAGGTGCTTTTAATGAGGAAAATCCATATTATATGGGTATTTACGATGGGAAAATT
GCCGAAGATAAAATACGAGATTATGTGGACAACAGCGATTTAATTTTAAATATTGGAGCC
AAATTAACAGATTCAGCAACAGCAGGTTTTTCATACCAATTCAATATCGATGATGTCGTT
ATGTTAAATCATCACAATATCAAAATTGACGATGTTACAAATGATGAAATATCTCTACCA
TCATTGTTAAAACAGTTATCCAATATTTCATATACGAATAACGCAACGTTCCCTGCGTAT
CATCGTCCAACATCACCCGATTATACTGTTGGCACAGAACCATTAACACAACAAACTTAT
TTTAAAATGATGCAAAATTTCTTAAAACCAAATGATGTCATCATTGCTGATCAAGGTACA
TCATTCTTTGGTGCTTATGATTTAGCATTATACAAAAACAATACTTTTATAGGGCAACCG
TTATGGGGTTCTATCGGCTATACATTACCTGCAACATTAGGTTCACAATTAGCAGACAAA
GATCGTCGTAACTTATTATTAATTGGTGATGGCTCATTGCAACTAACTGTTCAAGCTATT
TCAACTATGATTAGACAGCATATTAAACCGGTATTATTTGTGATTAATAATGACGGCTAT
ACGGTAGAACGACTTATTCACGGCATGTATGAACCTTATAATGAAATTCACATGTGGGAT
TATAAAGCTTTACCAGCTGTATTTGGTGGTAAAAATGTTGAAATTCATGACGTTGAATCA
TCAAAAGATTTACAAGACACGTTTAATGCAATTAATGGTCATCCCGATGTGATGCATTTT
GTCGAAGTCAAAATGGCTGTCGAAGACGCACCGAAGAAACTCATCGATATCGCTAAAGCT
TTTTCACAACAAAATAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS00995 [old locus tag: SAUSA300_0190 ]
- symbol: SAUSA300_RS00995
- description: indolepyruvate decarboxylase
- length: 546
- theoretical pI: 5.0203
- theoretical MW: 60538
- GRAVY: -0.171429
⊟Function[edit | edit source]
- reaction: EC 4.1.1.74? ExPASyIndolepyruvate decarboxylase 3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2
- TIGRFAM: Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 528.9)and 11 moreindolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 197.2)Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 159.9)Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 99.9)Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 88.8)glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 78.8)Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 77.1)Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 63.3)Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 49.2)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 41.9)sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 18.8)Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 13.9)
- TheSEED: see SAUSA300_0190
- PFAM: THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 118)and 3 moreFAD_DHS (CL0085) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 84.7)THDP-binding (CL0254) TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 59.7)FAD_DHS (CL0085) CO_dh; CO dehydrogenase beta subunit/acetyl-CoA synthase epsilon subunit (PF02552; HMM-score: 14.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010937
- TAT(Tat/SPI): 0.000972
- LIPO(Sec/SPII): 0.001253
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446736299 NCBI
- RefSeq: WP_000813555 NCBI
- UniProt: see SAUSA300_0190
⊟Protein sequence[edit | edit source]
- MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDWVGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPTRAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRPVHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSFHLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLILNIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNNATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYKNNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKPVLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTFNAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS01155 formate acetyltransferase [1] (data from MRSA252) SAUSA300_RS02025 alkyl hydroperoxide reductase subunit C [1] (data from MRSA252) SAUSA300_RS04560 NADH dehydrogenase [1] (data from MRSA252) SAUSA300_RS05350 pyruvate dehydrogenase E1 component subunit alpha [1] (data from MRSA252) SAUSA300_RS06220 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_RS08840 trigger factor [1] (data from MRSA252) SAUSA300_RS08950 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) SAUSA300_RS08975 pyruvate kinase [1] (data from MRSA252) SAUSA300_RS09165 formate--tetrahydrofolate ligase [1] (data from MRSA252) SAUSA300_RS11985 30S ribosomal protein S9 [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12140 50S ribosomal protein L23 [1] (data from MRSA252) SAUSA300_RS14290 ornithine carbamoyltransferase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CcpA see SAUSA300_0190
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)