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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS00330 [old locus tag: SAUSA300_0065 ]
- pan locus tag?: SAUPAN000814000
- symbol: SAUSA300_RS00330
- pan gene symbol?: arcA
- synonym:
- product: arginine deiminase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS00330 [old locus tag: SAUSA300_0065 ]
- symbol: SAUSA300_RS00330
- product: arginine deiminase
- replicon: chromosome
- strand: -
- coordinates: 73113..74348
- length: 1236
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (73113..74348) NCBI
- BioCyc: SAUSA300_RS00330 BioCyc
- MicrobesOnline: see SAUSA300_0065
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGGTACAAGGACCCATTCAAGTAAATAGTGAAATAGGCAAATTGAAAACTGTGTTGTTA
AAAAGACCAGGAAAAGAATTAGAAAATTTAGTACCTGATCATTTAAGTGGTTTATTATTC
GATGATATTCCCTACTTAAAAGTTGCACAAGAAGAGCATGACAAATTTGCTCAAACTTTG
AGAGATGAAGGAATCGAAGTAGTTTATTTAGAAAAACTTGCAGCAGAATCTATTACTGAG
CCAGAAGTACGCGAGAACTTCATAAACGACATATTAACAGAATCTAAAAAGACAATATTA
GGTCATGAAACTGAAATTAAAGAATTCTTTTCAAAGTTATCTGACCAAGAACTTGTAAAT
AAAATCATGGCTGGCGTACGTAAAGAAGAAATTCAACTTGAAACAACCCATTTAGTAGAA
TATATGGATGATAGATATCCATTTTACTTAGATCCAATGCCCAACCTTTATTTTACAAGA
GATCCCCAAGCTTCAATTGGTAGAGGAATGACAATTAACAGAATGTATTGGAGAGCACGA
CGTAGAGAATCTATTTTTATGACATATATACTGAAACATCATCCAAGATTTAAAGATAAA
GATGTACCAGTATGGTTAGATCGTAACTCACCATTTAATATTGAAGGTGGAGATGAATTA
GTATTATCGAAAGATGTTTTAGCTATTGGTATATCAGAACGTACATCAGCTCAAGCAATA
GAAAAGTTAGCACGTAATATTTTCAAAGATGCAAACACAAGTTTTAAAAAAATCGTAGCT
ATTGAAATACCTAATACACGTACATTTATGCACCTAGATACAGTACTAACTATGATTGAC
TACGATAAGTTTACAGTACATGCAGCAATATTTAAAGAAGAAAATAATATGAATATATTT
ACCATAGAACAAAATGATGGTAAGGACGATATAAAAATTACTCGTTCTAGCAAGTTACGT
GAAACACTTGCTGAAGTTTTAGAAGTAGAAAAAGTGGACTTTATTCCAACAGGTAATGGC
GACGTTATTGATGGTGCACGTGAACAATGGAATGATGGCTCAAACACATTATGTATTCGA
CCAGGGGTTGTGGTGACATACGATCGCAACTATGTATCAAACCAACTTTTACGCGACAAA
GGAATTAAAGTGATTGAAATTACTGGTAGTGAACTTGTACGTGGACGCGGAGGCCCAAGA
TGTATGAGTCAGCCGTTATTTAGAGAAGATATTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS00330 [old locus tag: SAUSA300_0065 ]
- symbol: SAUSA300_RS00330
- description: arginine deiminase
- length: 411
- theoretical pI: 5.18702
- theoretical MW: 47270.7
- GRAVY: -0.42944
⊟Function[edit | edit source]
- reaction: EC 3.5.3.6? ExPASyArginine deiminase L-arginine + H2O = L-citrulline + NH3
- TIGRFAM: Energy metabolism Amino acids and amines arginine deiminase (TIGR01078; EC 3.5.3.6; HMM-score: 558.9)and 1 morePurines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 12.5)
- TheSEED: see SAUSA300_0065
- PFAM: GME (CL0197) Amidinotransf; Amidinotransferase (PF02274; HMM-score: 390.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001251
- TAT(Tat/SPI): 0.000092
- LIPO(Sec/SPII): 0.000202
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446171949 NCBI
- RefSeq: WP_000249804 NCBI
- UniProt: see SAUSA300_0065
⊟Protein sequence[edit | edit source]
- MVQGPIQVNSEIGKLKTVLLKRPGKELENLVPDHLSGLLFDDIPYLKVAQEEHDKFAQTLRDEGIEVVYLEKLAAESITEPEVRENFINDILTESKKTILGHETEIKEFFSKLSDQELVNKIMAGVRKEEIQLETTHLVEYMDDRYPFYLDPMPNLYFTRDPQASIGRGMTINRMYWRARRRESIFMTYILKHHPRFKDKDVPVWLDRNSPFNIEGGDELVLSKDVLAIGISERTSAQAIEKLARNIFKDANTSFKKIVAIEIPNTRTFMHLDTVLTMIDYDKFTVHAAIFKEENNMNIFTIEQNDGKDDIKITRSSKLRETLAEVLEVEKVDFIPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNQLLRDKGIKVIEITGSELVRGRGGPRCMSQPLFREDI
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: ArcR, ArgR, CcpA, Rex see SAUSA300_0065
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.