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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2404 [new locus tag: SAUSA300_RS13315 ]
- pan locus tag?: SAUPAN006018000
- symbol: SAUSA300_2404
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2404 [new locus tag: SAUSA300_RS13315 ]
- symbol: SAUSA300_2404
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2588435..2590012
- length: 1578
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914612 NCBI
- RefSeq: YP_495039 NCBI
- BioCyc: see SAUSA300_RS13315
- MicrobesOnline: 1293919 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1561ATGACGTTTCTTACAGTCATGCAATTTATAGTTAACATTATCGTTGTAGGATTCATGCTT
ACGGTTATTGTTATCGGGCTTATTTGGTTAATTAAAGATAAAAGACAATCACAACATAGT
GTATTAAGGAATTATCCTTTACTAGCACGTATTAGATATATTTCAGAAAAAATGGGACCG
GAATTACGTCAGTATTTATTTTCTGGGGATAATGAAGGGAAACCTTTTTCACGTAATGAT
TATAAAAATATCGTTTTGGCTGGAAAATATAACTCTCGTATGACCAGCTTCGGTACTACT
AAAGATTATCAAGACGGCTTTTACATACAGAACACAATGTTTCCGATGCAACGTAATGAG
ATTTCAGTAGATAATACAACATTGTTATCAACATTCATTTATAAAATCGCGAATGAGCGT
TTATTTAGTCGTGAAGAATATCGTGTGCCGACAAAGATTGATCCGTATTACTTAAGTGAT
GACCATGCAATAAAATTAGGTGAACATTTAAAACATCCATTTATTTTAAAACGTATCGTA
GGACAATCTGGTATGAGTTATGGCGCTTTAGGAAAAAATGCCATTACAGCTTTATCTAAA
GGTCTAGCTAAAGCGGGCACTTGGATGAATACAGGTGAAGGTGGCTTATCAGAATATCAT
TTAAAAGGTAATGGGGATATCATTTTCCAAATTGGTCCCGGTTTATTTGGTGTTCGTGAT
AAAGAAGGTAATTTTAGTGAAGGTTTATTTAAAGAGGTTGCACAGTTATCTAACGTACGC
GCATTTGAGCTGAAGTTGGCACAAGGTGCTAAGACTCGTGGTGGTCATATGGAAGCTGAA
AAGGTAAACGAAGAAATTGCTAAAATCCGAAATGTTGAACCTTATAAAACAATCAATTCA
CCTAACCGTTACGAATTTATTCATAATGCTGAAGATTTGATTCGTTTCGTCGATCAGTTG
CAGCAATTAGGTCAAAAACCAGTAGGATTCAAAATTGTAGTAAGCAAAGTTTCAGAAATT
GAAACACTTGTACGTACGATGGTGGAACTAGATAAGTATCCAAGCTTTATTACGATTGAT
GGTGGTGAAGGTGGTACTGGTGCAACATTCCAAGAATTACAAGATGGTGTTGGCTTACCG
CTATTTACAGCTCTACCTATTGTGTCTGGCATGTTAGAAAAATATGGTATTCGAGATAAA
GTGAAATTGGCGGCATCTGGTAAGTTAGTGACACCAGATAAAATTGCGATTGCACTAGGT
TTAGGTGCAGATTTTGTAAATATCGCACGTGGGATGATGATTAGTGTCGGTTGTATAATG
AGTCAACAATGTCACATGAATACGTGTCCTGTAGGTGTTGCAACGACAGATGCGAAGAAA
GAAAAAGCATTGATTGTTGGAGAAAAGCAATATCGTGTCACAAACTATGTAACAAGTTTG
CATGAAGGCTTATTCAATATTGCAGCAGCTGTTGGCGTATCCAGTCCTACAGAAATTACT
GCTGATCATATTGTATATCGAAAAGTCGATGGTGAGTTACAAACGATACATGATTATAAA
TTAAAACTCATTAGTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2404 [new locus tag: SAUSA300_RS13315 ]
- symbol: SAUSA300_2404
- description: hypothetical protein
- length: 525
- theoretical pI: 9.24714
- theoretical MW: 58439.1
- GRAVY: -0.122857
⊟Function[edit | edit source]
- TIGRFAM: PfaD family protein (TIGR02814; HMM-score: 12.8)Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 10.9)
- TheSEED :
- Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)
Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)and 1 more - PFAM: TIM_barrel (CL0036) Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 332.5)and 2 moreGlu_syn_central; Glutamate synthase central domain (PF04898; HMM-score: 18.7)IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 15.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helix: 1
- LocateP: N-terminally anchored (No CS)
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 4
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002678
- TAT(Tat/SPI): 0.000389
- LIPO(Sec/SPII): 0.001608
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRNYPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKDYQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSDDHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSEYHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGHMEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVVSKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGMLEKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCPVGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRKVDGELQTIHDYKLKLIS
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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