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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2469 [new locus tag: SACOL_RS12945 ]
  • pan locus tag?: SAUPAN006018000
  • symbol: SACOL2469
  • pan gene symbol?: —
  • synonym:
  • product: glutamate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2469 [new locus tag: SACOL_RS12945 ]
  • symbol: SACOL2469
  • product: glutamate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 2524874..2526451
  • length: 1578
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGACGTTTCTTACAGTCATGCAATTTATAGTTAACATTATCGTTGTAGGATTCATGCTT
    ACGGTTATTGTTATCGGGCTTATTTGGTTAATTAAAGATAAAAGACAATCACAACATAGT
    GTATTAAGGAATTATCCTTTACTAGCACGTATTAGATATATTTCAGAAAAAATGGGACCG
    GAATTACGTCAGTATTTATTTTCTGGGGATAATGAAGGGAAACCTTTTTCACGTAATGAT
    TATAAAAATATCGTTTTGGCTGGAAAATATAACTCTCGTATGACCAGCTTCGGTACTACT
    AAAGATTATCAAGACGGCTTTTACATACAGAACACAATGTTTCCGATGCAACGTAATGAG
    ATTTCAGTAGATAATACAACATTGTTATCAACATTCATTTATAAAATCGCGAATGAGCGT
    TTATTTAGTCGTGAAGAATATCGTGTGCCGACAAAGATTGATCCGTATTACTTAAGTGAT
    GACCATGCAATAAAATTAGGTGAACATTTAAAACATCCATTTATTTTAAAACGTATCGTA
    GGACAATCTGGTATGAGTTATGGCGCTTTAGGAAAAAATGCCATTACAGCTTTATCTAAA
    GGTCTAGCTAAAGCGGGCACTTGGATGAATACAGGTGAAGGTGGCTTATCAGAATATCAT
    TTAAAAGGTAATGGGGATATCATTTTCCAAATTGGTCCCGGTTTATTTGGTGTTCGTGAT
    AAAGAAGGTAATTTTAGTGAAGGTTTATTTAAAGAGGTTGCACAGTTATCTAACGTACGC
    GCATTTGAGCTGAAGTTGGCACAAGGTGCTAAGACTCGTGGTGGTCATATGGAAGCTGAA
    AAGGTAAACGAAGAAATTGCTAAAATCCGAAATGTTGAACCTTATAAAACAATCAATTCA
    CCTAACCGTTACGAATTTATTCATAATGCTGAAGATTTGATTCGTTTCGTCGATCAGTTG
    CAGCAATTAGGTCAAAAACCAGTAGGATTCAAAATTGTAGTAAGCAAAGTTTCAGAAATT
    GAAACACTTGTACGTACGATGGTGGAACTAGATAAGTATCCAAGCTTTATTACGATTGAT
    GGTGGTGAAGGTGGTACTGGTGCAACATTCCAAGAATTACAAGATGGTGTTGGCTTACCG
    CTATTTACAGCTCTACCTATTGTGTCTGGCATGTTAGAAAAATATGGTATTCGAGATAAA
    GTGAAATTGGCGGCATCTGGTAAGTTAGTGACACCAGATAAAATTGCGATTGCACTAGGT
    TTAGGTGCAGATTTTGTAAATATCGCACGTGGGATGATGATTAGTGTCGGTTGTATAATG
    AGTCAACAATGTCACATGAATACGTGTCCTGTAGGTGTTGCAACGACAGATGCGAAGAAA
    GAAAAAGCATTGATTGTTGGAGAAAAGCAATATCGTGTCACAAACTATGTAACAAGTTTG
    CATGAAGGCTTATTCAATATTGCAGCAGCTGTTGGCGTATCCAGTCCTACAGAAATTACT
    GCTGATCATATTGTATATCGAAAAGTCGATGGTGAGTTACAAACGATACATGATTATAAA
    TTAAAACTCATTAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1578

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2469 [new locus tag: SACOL_RS12945 ]
  • symbol: SACOL2469
  • description: glutamate synthase
  • length: 525
  • theoretical pI: 9.24714
  • theoretical MW: 58439.1
  • GRAVY: -0.122857

⊟Function[edit | edit source]

  • TIGRFAM:
    PfaD family protein (TIGR02814; HMM-score: 12.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other isopentenyl-diphosphate delta-isomerase, type 2 (TIGR02151; EC 5.3.3.2; HMM-score: 10.9)
  • TheSEED  :
    • Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis  Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)
    and 1 more
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Ammonia assimilation  Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)
  • PFAM:
    TIM_barrel (CL0036) Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 332.5)
    and 2 more
    Glu_syn_central; Glutamate synthase central domain (PF04898; HMM-score: 18.7)
    IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 15.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002678
    • TAT(Tat/SPI): 0.000389
    • LIPO(Sec/SPII): 0.001608
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTFLTVMQFIVNIIVVGFMLTVIVIGLIWLIKDKRQSQHSVLRNYPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKDYQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSDDHAIKLGEHLKHPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSEYHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGHMEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVVSKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGMLEKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCPVGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRKVDGELQTIHDYKLKLIS

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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