PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2389 [new locus tag: SAUSA300_RS13225 ]
• pan locus tag^?: SAUPAN005996000
• symbol: SAUSA300_2389
• pan gene symbol^?: —
• synonym:
• product: putative drug transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2389 [new locus tag: SAUSA300_RS13225 ]
• symbol: SAUSA300_2389
• product: putative drug transporter
• replicon: chromosome
• strand: -
• coordinates: 2571924..2573324
• length: 1401
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913432 NCBI
• RefSeq: YP_495024 NCBI
• BioCyc: see SAUSA300_RS13225
• MicrobesOnline: 1293904 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
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  1261
  1321
  1381
  
  ATGGACACATCAAAACAATTTAGAGGTGACAACCGATTGCTTTTGGGTATCGTTTTAGGG
  GTTATTACCTTTTGGCTATTCGCGCAGTCACTTGTTAATCTTGTTGTCCCATTACAATCA
  ACATATAGTAGTGACGTTGGAACGATAAATATCGCTGTTAGCTTATCTGCCTTATTTGCT
  GGTTTGTTTATCGTAGGTGCTGGTGATGTTGCTGATAAATTTGGTCGCGTCAAAATTACT
  TATGTAGGATTGATATTAAATGTTGTAGGTTCATTACTCATCATCATTACACCTTTGCCA
  GCATTTTTAATTATAGGTAGAATAATTCAAGGTTTGTCTGCAGCATGTATTATGCCATCA
  ACACTTGCTATTATTAACGAATATTATATTGGTACAAGAAGACAACGTGCCTTAAGCTAT
  TGGTCTATTGGTTCTTGGGGTGGTAGTGGTATTTGTACGTTGTTTGGTGGCTTAATGGCT
  ACATATATAGGTTGGCGTTCAATATTTGTTGTTTCAATTCTATTAACATTATTAGCAATG
  TACTTAATCAAACATGCACCTGAGACTAAAGCAGAACCAATCAAAGGTATGAAAGCAGAA
  GCTAAAAAGTTTGACGTTATTGGTTTAGTCATTTTAGTAGTGACGATGTTAAGTTTAAAT
  GTAATCATCACACAGACGTCTCATTTTGGTTTAGTTTCACCGTTAATTCTAGGTTTAATT
  GTTGTGTTTATCTGTTCATTAGTTGGTTTTGTATATTACGAAAATAAAATAAAGCATCCA
  CTTGTTGATTTTTCAATTTTTAAAAATAGAGGATACAGTGGTGCAACTATTTCAAACTTC
  TTATTAAATGGTGTAGCAGGTGGTGCACTTATCGTTATTAACACGTATTATCAACAACAA
  TTAGGATTTAATTCTTCGCAAACGGGTTATATTTCATTAACGTATTTAATAACAGTGTTG
  TCAATGATTCGTGTAGGTGAAAAGATTTTATCTCAACATGGTCCGAAGCGCCCACTATTA
  CTAGGAAGTGGCTTTACAGTGATTGGGTTAATCTTATTGTCGTTAACATTTTTACCAGAA
  GTGTGGTATATCATATCTAGTATAGTTGGATATTTATTGTTTGGTACTGGTTTAGGATTA
  TATGCTACACCATCAACTGATACAGCAGTTGCTAGTGCGCCAGATGATAAGTCGGGTGTT
  GCTTCAGGTGTGTATAAAATGGCGTCATCATTAGGAAATGCATTTGGAGTAGCAGTATCT
  GGTACGGTTTATACTGTGTTAGCAGCTAATTTAAATTTGAACTTAGGTGGTTTCACAGGT
  ATGATGTTTAATGCCTTGCTAGCAATTGTTGCATTTTTAGTCATTTTACTATTAGTTCCT
  AAAAATCAAACGAATTTGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2389 [new locus tag: SAUSA300_RS13225 ]
• symbol: SAUSA300_2389
• description: putative drug transporter
• length: 466
• theoretical pI: 9.74613
• theoretical MW: 49840.8
• GRAVY: 0.840987

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 123.8)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 123.8)
  and 9 more
  multidrug resistance protein (TIGR00880; HMM-score: 61.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 46.9)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 46)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 35.7)
  Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 30.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 29.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 24.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 20.3)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 17.1)
• TheSEED  :
  • putative transporter
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 159.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9987
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0013
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.066585
  • TAT(Tat/SPI): 0.002994
  • LIPO(Sec/SPII): 0.021327
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 87160731 NCBI
• RefSeq: YP_495024 NCBI
• UniProt: A0A0H2XH56 UniProt

⊟Protein sequence

• MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSSDVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAFLIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMATYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLSLNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGATISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHGPKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVASAPDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIVAFLVILLLVPKNQTNL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications