PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2388 [new locus tag: SAUSA300_RS13220 ]
• pan locus tag^?: SAUPAN005995000
• symbol: panE
• pan gene symbol^?: —
• synonym:
• product: 2-dehydropantoate 2-reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2388 [new locus tag: SAUSA300_RS13220 ]
• symbol: panE
• product: 2-dehydropantoate 2-reductase
• replicon: chromosome
• strand: -
• coordinates: 2570763..2571698
• length: 936
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914289 NCBI
• RefSeq: YP_495023 NCBI
• BioCyc: see SAUSA300_RS13220
• MicrobesOnline: 1293903 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAAATTGCAATTGCTGGATCGGGTGCATTAGGTAGTGGCTTTGGTGCCAAACTATTT
  CAAGCAGGATATGATGTCACACTTATTGACGGATATACATCTCATGTTGAAGCGGTTAAG
  CAACATGGATTAAATATAACGATTAATGGAGAGGCATTCGAGTTAAACATTCCGATGTAT
  CATTTTAATGATCAACCGGACGAAAGCATTTACGATGTTGTCTTTCTATTTCCAAAGTCT
  ATGCAATTAAAAGAAGTGATGGAAGATATGAAGCCACATATTGATAATGAAACGATCGTC
  GTATGTACGATGAATGGTCTGAAGCATGAAGAAGTCATTGCGCAGTATGTTGCTCAATCA
  CAAATTGTCAGAGGTGTTACGACTTGGACGGCAGGTCTTGAAAGCCCTGGACACAGTCAT
  TTACTTGGTAGTGGACCAGTTGAAATAGGTGAACTAGTGGATGAAGGTAAAGAAAATGTT
  ATAAAAGTTGCTGATTTACTTAACGAAGCGGAATTGAATGGTGTCATTAGTAAAGATTTA
  TACCAATCGATTTGGAAAAAGATTTGTGTTAATGGTACGGCAAATGCATTAAGCACAGTG
  TTGGAGTGTAATATGGCATCGCTGAATGAAAGTAGTTATGCGAAGTGTTTGATTTATAAA
  TTAACGCAAGAAATAGTGCATGTAGCGACGATTGATAATGTTCATTTAAATGTTGATGAA
  GTATTTGAATATTTAGTTGATTTAAATGAAAAAGTTGGTGCGCATTATCCATCCATGTAT
  CAAGATTTAATTGTTAATAATAGAAAAACTGAAATTGATTATATTAATGGCGCAGTTGCA
  ACATTAGGTAAACAACGTCATATTGAAGCGCCAGTCAATCGCTTTATTACTGATTTAATT
  CATACTAAAGAAAGTCAGCGACACGCACAGGATTAA
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  936
  

⊟Protein

⊟General

• locus tag: SAUSA300_2388 [new locus tag: SAUSA300_RS13220 ]
• symbol: PanE
• description: 2-dehydropantoate 2-reductase
• length: 311
• theoretical pI: 4.7892
• theoretical MW: 34443.9
• GRAVY: -0.117363

⊟Function

• reaction:
  EC 1.1.1.169?  ExPASy
  2-dehydropantoate 2-reductase (R)-pantoate + NADP⁺ = 2-dehydropantoate + NADPH
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 306)
  and 4 more
  Cellular processes Cellular processes Toxin production and resistance tellurite resistance protein TehB (TIGR00477; HMM-score: 16.8)
  Metabolism Energy metabolism Electron transport NADPH-dependent F420 reductase (TIGR01915; HMM-score: 15.3)
  nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 14.6)
  selenium-dependent molybdenum hydroxylase system protein, YqeB family (TIGR03309; HMM-score: 11.6)
• TheSEED  :
  • 2-dehydropantoate 2-reductase (EC 1.1.1.169)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  2-dehydropantoate 2-reductase (EC 1.1.1.169)
• PFAM:
  NADP_Rossmann (CL0063) ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 130.4)
  6PGD_C (CL0106) ApbA_C; Ketopantoate reductase PanE/ApbA C terminal (PF08546; HMM-score: 113.4)
  and 9 more
  NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 24.8)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 22.8)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 20.6)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20)
  no clan defined DUF4172; Domain of unknown function (DUF4172) (PF13776; HMM-score: 17.7)
  NADP_Rossmann (CL0063) UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 16.7)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 15.7)
  TehB; Tellurite resistance protein TehB (PF03848; HMM-score: 13.5)
  FAD_binding_3; FAD binding domain (PF01494; HMM-score: 11.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.129979
  • TAT(Tat/SPI): 0.002403
  • LIPO(Sec/SPII): 0.031557
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162032 NCBI
• RefSeq: YP_495023 NCBI
• UniProt: A0A0H2XJU5 UniProt

⊟Protein sequence

• MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGLNITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVCTMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENVIKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLIYKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYINGAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications