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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2448 [new locus tag: SACOL_RS12850 ]
  • pan locus tag?: SAUPAN005995000
  • symbol: SACOL2448
  • pan gene symbol?: —
  • synonym:
  • product: 2-dehydropantoate 2-reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2448 [new locus tag: SACOL_RS12850 ]
  • symbol: SACOL2448
  • product: 2-dehydropantoate 2-reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2507202..2508137
  • length: 936
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAATTGCAATTGCTGGATCGGGTGCATTAGGTAGTGGCTTTGGTGCCAAACTATTT
    CAAGCAGGATATGATGTCACACTTATTGACGGATATACATCTCATGTTGAAGCGGTTAAG
    CAACATGGATTAAATATAACGATTAATGGAGAGGCATTCGAGTTAAACATTCCGATGTAT
    CATTTTAATGATCAACCGGACGAAAGCATTTACGATGTTGTCTTTCTATTTCCAAAGTCT
    ATGCAATTAAAAGAAGTGATGGAAGATATGAAGCCACATATTGATAATGAAACGATCGTC
    GTATGTACGATGAATGGTCTGAAGCATGAAGAAGTCATTGCGCAGTATGTTGCTCAATCA
    CAAATTGTCAGAGGTGTTACGACTTGGACGGCAGGTCTTGAAAGCCCTGGACACAGTCAT
    TTACTTGGTAGTGGACCAGTTGAAATAGGTGAACTAGTGGATGAAGGTAAAGAAAATGTT
    ATAAAAGTTGCTGATTTACTTAACGAAGCGGAATTGAATGGTGTCATTAGTAAAGATTTA
    TACCAATCGATTTGGAAAAAGATTTGTGTTAATGGTACGGCAAATGCATTAAGCACAGTG
    TTGGAGTGTAATATGGCATCGCTGAATGAAAGTAGTTATGCGAAGTGTTTGATTTATAAA
    TTAACGCAAGAAATAGTGCATGTAGCGACGATTGATAATGTTCATTTAAATGTTGATGAA
    GTATTTGAATATTTAGTTGATTTAAATGAAAAAGTTGGTGCGCATTATCCATCCATGTAT
    CAAGATTTAATTGTTAATAATAGAAAAACTGAAATTGATTATATTAATGGCGCAGTTGCA
    ACATTAGGTAAACAACGTCATATTGAAGCGCCAGTCAATCGCTTTATTACTGATTTAATT
    CATACTAAAGAAAGTCAGCGACACGCACAGGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2448 [new locus tag: SACOL_RS12850 ]
  • symbol: SACOL2448
  • description: 2-dehydropantoate 2-reductase
  • length: 311
  • theoretical pI: 4.7892
  • theoretical MW: 34443.9
  • GRAVY: -0.117363

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.169?  ExPASy
    2-dehydropantoate 2-reductase (R)-pantoate + NADP+ = 2-dehydropantoate + NADPH
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 306)
    and 4 more
    Cellular processes Cellular processes Toxin production and resistance tellurite resistance protein TehB (TIGR00477; HMM-score: 16.8)
    Metabolism Energy metabolism Electron transport NADPH-dependent F420 reductase (TIGR01915; HMM-score: 15.3)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 14.6)
    selenium-dependent molybdenum hydroxylase system protein, YqeB family (TIGR03309; HMM-score: 11.6)
  • TheSEED  :
    • 2-dehydropantoate 2-reductase (EC 1.1.1.169)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  2-dehydropantoate 2-reductase (EC 1.1.1.169)
  • PFAM:
    NADP_Rossmann (CL0063) ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 130.4)
    6PGD_C (CL0106) ApbA_C; Ketopantoate reductase PanE/ApbA C terminal (PF08546; HMM-score: 113.4)
    and 9 more
    NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 24.8)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 22.8)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 20.6)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 20)
    no clan defined DUF4172; Domain of unknown function (DUF4172) (PF13776; HMM-score: 17.7)
    NADP_Rossmann (CL0063) UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 16.7)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 15.7)
    TehB; Tellurite resistance protein TehB (PF03848; HMM-score: 13.5)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 11.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.129979
    • TAT(Tat/SPI): 0.002403
    • LIPO(Sec/SPII): 0.031557
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIAIAGSGALGSGFGAKLFQAGYDVTLIDGYTSHVEAVKQHGLNITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEDMKPHIDNETIVVCTMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENVIKVADLLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLIYKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYINGAVATLGKQRHIEAPVNRFITDLIHTKESQRHAQD

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 192 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 20.71 h [5]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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