PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2339 [new locus tag: SAUSA300_RS12915 ]
• pan locus tag^?: SAUPAN005924000
• symbol: SAUSA300_2339
• pan gene symbol^?: nreA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2339 [new locus tag: SAUSA300_RS12915 ]
• symbol: SAUSA300_2339
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2513533..2513985
• length: 453
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913614 NCBI
• RefSeq: YP_494974 NCBI
• BioCyc: see SAUSA300_RS12915
• MicrobesOnline: 1293854 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  TTGACACCAGAAGCAATAATTGAAGATCGTAGATTTCAAGAGACATTAGATAAAATACGC
  AAAGAAGAGGGTTATGATTTTGCGGCAATCGCTTTCTATGAATCTAACAAACCATCATCT
  CCAATAAAATGGCACTATGTTTCAGGTAATAAAAATAATCGATTTAAATTGATTATTTTA
  AGAAAAGGTCGTGGATTAGCTGGAACTGTTATGAAAACAGGAAAGCGTATGGTAATAGCC
  AATGTTGGTTTAGCTTTAGGGCCTGAAGAAAAGATTGACTATCCAATTTTATTGAGCGAA
  TCATTAACAGCAGTTTTAGCAGTGCCGCTTTGGTATAAGAATCAAGTGTATGGCGTACTA
  TTATTTGGTCAAAGAGATGGTCGTCCTTTACCAAAAATATTTGATAATGATGATATTCAA
  CGTAAGTTTGGTATTTTCAATGATGATAAATAA
  60
  120
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  420
  453
  

⊟Protein

• 4PAU
  PDB

Protein Data Bank: 4PAU

⊟General

• locus tag: SAUSA300_2339 [new locus tag: SAUSA300_RS12915 ]
• symbol: SAUSA300_2339
• description: hypothetical protein
• length: 150
• theoretical pI: 9.92029
• theoretical MW: 17149.7
• GRAVY: -0.381333

⊟Function

• TIGRFAM:
  Metabolism Central intermediary metabolism Nitrogen fixation Nif-specific regulatory protein (TIGR01817; HMM-score: 11.6)
  Signal transduction Regulatory functions DNA interactions Nif-specific regulatory protein (TIGR01817; HMM-score: 11.6)
• TheSEED  :
  • Oxygen-sensing, dissimilatory nitrate and nitrite reduction regulatory protein NreA
• PFAM:
  GAF (CL0161) GAF_2; GAF domain (PF13185; HMM-score: 34.5)
  and 2 more
  GAF; GAF domain (PF01590; HMM-score: 26.6)
  GAF_3; GAF domain (PF13492; HMM-score: 20.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.32
  • Cytoplasmic Membrane Score: 9.55
  • Cellwall Score: 0.12
  • Extracellular Score: 0.01
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008644
  • TAT(Tat/SPI): 0.003867
  • LIPO(Sec/SPII): 0.002183
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162305 NCBI
• RefSeq: YP_494974 NCBI
• UniProt: A0A0H2XKD5 UniProt

⊟Protein sequence

• MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKWHYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESLTAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2337 < SAUSA300_2338 < SAUSA300_2339 < narI < narJ < narH < SAUSA300_2343

⊟Regulation

• regulators: NreC* (activation) regulon, Rex (repression) regulon

  NreC*	(TF)	important in Nitrate and nitrite respiration; RegPrecise    transcription unit transferred from N315 data RegPrecise
  Rex	(TF)	important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications