From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2253 [new locus tag: SAUSA300_RS12440 ]
  • pan locus tag?: SAUPAN005772000
  • symbol: ssaA
  • pan gene symbol?: —
  • synonym:
  • product: secretory antigen precursor SsaA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2253 [new locus tag: SAUSA300_RS12440 ]
  • symbol: ssaA
  • product: secretory antigen precursor SsaA
  • replicon: chromosome
  • strand: +
  • coordinates: 2419919..2420419
  • length: 501
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAAAAATTAGTAACAGCAACTACGTTAACAGCAGGAATCGGCACAGCATTAGTAGGT
    CAAGCACATCATGCAGATGCTGCTGAAAATTATACAAATTACAACAACTATAACTACAAC
    ACGACTCAAACTACAACGACTACGACAACTACGACAACTACATCATCAATTTCACATTCT
    GGTAACTTATACACTGCAGGACAATGTACTTGGTATGTATATGATAAAGTTGGCGGAGAA
    ATCGGTTCTACTTGGGGAAATGCTAATAATTGGGCTGCTGCTGCACAAGGTGCTGGATTC
    ACAGTAAATCATACACCTTCTAAAGGCGCTATCCTACAATCTTCTGAAGGACCATTTGGT
    CACGTTGCATATGTAGAAAGTGTAAACAGTGATGGTTCAGTTACAATTTCAGAAATGAAT
    TATAGTGGCGGACCTTTCTCAGTAAGTTCTAGAACTATTTCTGCAAGTGAAGCAGGTAAC
    TACAACTACATCCATATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    501

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2253 [new locus tag: SAUSA300_RS12440 ]
  • symbol: SsaA
  • description: secretory antigen precursor SsaA
  • length: 166
  • theoretical pI: 6.21564
  • theoretical MW: 17398.7
  • GRAVY: -0.351205

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Surface structures pilus (MSHA type) biogenesis protein MshL (TIGR02519; HMM-score: 5.4)
  • TheSEED  :
    • Secretory antigen SsaA-like protein SAV2304
  • PFAM:
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 53.4)
    and 1 more
    no clan defined Dicty_REP; Dictyostelium (Slime Mold) REP protein (PF05086; HMM-score: 4.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: HHADAAEN
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.996663
    • TAT(Tat/SPI): 0.001506
    • LIPO(Sec/SPII): 0.001376
    • Cleavage Site: CS pos: 27-28. ADA-AE. Pr: 0.9730
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKLVTATTLTAGIGTALVGQAHHADAAENYTNYNNYNYNTTQTTTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTVNHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGNYNYIHI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_2253&oldid=93236"