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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2249 [new locus tag: SAUSA300_RS12415 ]
- pan locus tag?: SAUPAN005767000
- symbol: ssaA
- pan gene symbol?: ssaA
- synonym:
- product: secretory antigen precursor SsaA
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2249 [new locus tag: SAUSA300_RS12415 ]
- symbol: ssaA
- product: secretory antigen precursor SsaA
- replicon: chromosome
- strand: +
- coordinates: 2415199..2416002
- length: 804
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3913090 NCBI
- RefSeq: YP_494884 NCBI
- BioCyc: see SAUSA300_RS12415
- MicrobesOnline: 1293764 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGAAGAAAATCGCTACAGCTACTATCGCAACTGCAGGATTCGCTACAATCGCAATTGCA
TCAGGAAATCAAGCTCATGCTTCTGAGCAAGATAACTACGGTTATAATCCAAACGACCCA
ACATCATATAGCTATACTTACACTATTGATGCACAAGGTAACTACCATTACACATGGAAA
GGTAACTGGCATCCAAGTCAATTAAACCAAGATAATGGCTACTACAGCTATTACTACTAC
AATGGTTACAATAACTATAACAATTACAACAACGGTTATAGCTACAATAACTACAGCCGT
TACAACAACTACTCAAATAATAATCAATCATATAACTACAATAACTATAATAGTTACAAC
ACAAACAGCTACCGTACTGGTGGTTTAGGTGCAAGCTACAGCACTTCAAGCAACAATGTT
CAAGTAACTACAACTATGGCTCCATCATCAAATGGCCGTTCAATCTCAAGTGGTTATACT
TCAGGACGTAACTTATACACTTCTGGTCAATGTACATACTACGTATTTGATCGTGTAGGT
GGTAAAATCGGTTCAACTTGGGGCAATGCAAGTAACTGGGCTAACGCAGCTGCAAGAGCT
GGTTACACAGTGAACAATACACCAAAAGCTGGTGCAATTATGCAAACAACTCAAGGTGCA
TACGGTCACGTTGCATACGTTGAAAGTGTTAACAGCAATGGTTCAGTAAGAGTTTCAGAA
ATGAACTATGGTTATGGCCCAGGTGTTGTAACTTCACGTACAATCTCAGCTAGCCAAGCT
GCTGGTTATAACTTCATTCACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2249 [new locus tag: SAUSA300_RS12415 ]
- symbol: SsaA
- description: secretory antigen precursor SsaA
- length: 267
- theoretical pI: 9.09862
- theoretical MW: 29327
- GRAVY: -0.846816
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.02
- Extracellular Score: 9.98
- Internal Helices: 0
- LocateP: Secretory(released) (with CS)
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: -0.17
- Signal peptide possibility: 1
- N-terminally Anchored Score: -2
- Predicted Cleavage Site: NQAHASEQ
- SignalP: Signal peptide SP(Sec/SPI) length 27 aa
- SP(Sec/SPI): 0.998381
- TAT(Tat/SPI): 0.000739
- LIPO(Sec/SPII): 0.000657
- Cleavage Site: CS pos: 27-28. AHA-SE. Pr: 0.9903
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYSYTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- data available for NCTC8325
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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