PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1705 [new locus tag: SAUSA300_RS09310 ]
• pan locus tag^?: SAUPAN004444000
• symbol: SAUSA300_1705
• pan gene symbol^?: —
• synonym:
• product: putative drug transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1705 [new locus tag: SAUSA300_RS09310 ]
• symbol: SAUSA300_1705
• product: putative drug transporter
• replicon: chromosome
• strand: -
• coordinates: 1887832..1889013
• length: 1182
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913762 NCBI
• RefSeq: YP_494399 NCBI
• BioCyc: see SAUSA300_RS09310
• MicrobesOnline: 1293220 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAATATACCTAAATCAGTCTGGTGGCTAGTAATTGGCATGGCGTTAAATATTACTGGT
  TCCAGTTTTTTGTGGCCTTTAAATACAATTTATATGAAACAAGAACTTGGAAAAAGTTTA
  ACTGTTGCTGGTTTAGTGCTAATGATAAATTCATTTGGCATGGTTATTGGAAACTTATTA
  GGTGGTTCACTATTTGATAAATTAGGTGGATACAAGACGATTTTAATTGGAACTTTCACT
  TGTCTTTGTAGTACAACGCTACTTAATTTCTTTCACGGGTGGCCTTGGTATGCTGTATGG
  CTTGTAATGTTAGGGTTTGGTGGCGGAATGATTATTCCTGCGATATACGCTATGGCTGGA
  GCAGTGTGGCCAAATGGCGGAAGACAAACGTTTAATGCGATATACTTAGCGCAAAATATT
  GGTGTGGCTGTCGGTGCTGCAATGGGCGGCTTTGTCGCAGAATTTAGCTTTAACTATATC
  TTTTTAGCCAATCTTATTATGTATGTTGTGTTTGCGCTTGTCGCGGTAACGCAATTTAAT
  ATTGAAATTAATGCGAAAGTTAAATATCCAACTCATTTAGATATTACTGGTAAAAAGAAT
  AAAGCAAGATTTATTTCATTAGTACTAATTTGTGCAATGTTTGCAATTTGTTGGGTTGCA
  TATATTCAATGGGAGTCTACAATCGCTTCATTTACACAATCTATTAATATTTCAATGGCA
  CAATATAGTGTTTTATGGACAATTAACGGAATAATGATTTTAGTAGCACAACCATTAATT
  AAACCGATTCTCTATCTGTTAAAAGGAAACTTAAAGAAGCAAATGTTTGTCGGCATCATC
  ATTTTTATGTTGTCGTTCTTTGTCACGAGTTTTGCCGAAAACTTTACAATATTTGTTGTC
  GGTATGATTATTTTAACTTTTGGAGAAATGTTTGTATGGCCAGCAGTTCCAACTATAGCC
  AATCAGTTAGCGCCAGATGGTAAGCAAGGACAGTACCAAGGTTTTGTGAATTCAGCTGCT
  ACAGTAGGAAAAGCATTTGGTCCATTTCTTGGTGGTGTATTAGTTGATGCGTTTAATATG
  CGCATGATGTTTATCGGTATGATGCTACTACTTGTATTTGCATTAATATTATTAATGGTT
  TTCAAGGAGAATAATACGCAACCTAAAAAAATAGATGCATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1705 [new locus tag: SAUSA300_RS09310 ]
• symbol: SAUSA300_1705
• description: putative drug transporter
• length: 393
• theoretical pI: 9.75148
• theoretical MW: 43344.8
• GRAVY: 0.840204

⊟Function

• TIGRFAM:
  multidrug resistance protein (TIGR00880; HMM-score: 72.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 69.8)
  and 8 more
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 48.1)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 48.1)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 31)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 30.5)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 27)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 26.8)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 21.8)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 4.3)
• TheSEED  :
  • Multidrug resistance protein
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 141.1)
  and 3 more
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 39.8)
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 12.8)
  no clan defined Trp_oprn_chp; Tryptophan-associated transmembrane protein (Trp_oprn_chp) (PF09534; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.032864
  • TAT(Tat/SPI): 0.000526
  • LIPO(Sec/SPII): 0.058627
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 87160224 NCBI
• RefSeq: YP_494399 NCBI
• UniProt: A0A0H2XG30 UniProt

⊟Protein sequence

• MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAGLVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLVMLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYIFLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICWVAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMFVGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQGFVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKIDA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications