PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1694 [new locus tag: SAUSA300_RS09255 ]
• pan locus tag^?: SAUPAN004425000
• symbol: trmB
• pan gene symbol^?: trmB
• synonym:
• product: tRNA (guanine-N(7)-)-methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1694 [new locus tag: SAUSA300_RS09255 ]
• symbol: trmB
• product: tRNA (guanine-N(7)-)-methyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1868313..1868957
• length: 645
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914186 NCBI
• RefSeq: YP_494388 NCBI
• BioCyc: see SAUSA300_RS09255
• MicrobesOnline: 1293209 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAGAGTTCGATACAAACCATGGGCTGAAGATTATTTAAAAGATCATCCTGAATTGGTT
  GACATGGACGGTCAACATGCAGGGAAAATGACTGAATGGTTTGATAAAACGCAACCGATA
  CACATTGAAATTGGTTCGGGTATGGGGCAGTTTATTACAACATTAGCTGCTCAAAATCCT
  CATATTAACTATATTTCAATGGAACGTGAGAAAAGTATCGTTTATAAAGTATTGGATAAA
  GTTAAAGAGATGGGTTTAACAAATTTAAAGATTATTTGTAATGACGCCATTGAATTAAAT
  GAATATTTTAAAGACGGCGAAGTTTCGCGTATTTATTTGAACTTTTCAGATCCATGGCCA
  AAAAATCGTCATGCAAAACGACGTTTAACGTATCATACGTTTTTAGCGTTGTATCAACAA
  ATCTTAAATGATGAAGGAGATTTACATTTTAAAACTGATAACAGAGGACTATTTGCCTAC
  AGTTTAGAAAGTATGTCTCAATTTGGAATGTATTTTACAAAAATAAATCTAAATTTACAT
  CAAGAAGACGATGGCAGTAATATTTTAACAGAATATGAAAAGAAATTTTCGGATAAAGGG
  TCACGTATTTATCGTATGGAAGCGAAATTCCATTCACAAAAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1694 [new locus tag: SAUSA300_RS09255 ]
• symbol: TrmB
• description: tRNA (guanine-N(7)-)-methyltransferase
• length: 214
• theoretical pI: 7.03221
• theoretical MW: 25275.5
• GRAVY: -0.707944

⊟Function

• reaction:
  EC 2.1.1.33?  ExPASy
  tRNA (guanine⁴⁶-N⁷)-methyltransferase S-adenosyl-L-methionine + guanine⁴⁶ in tRNA = S-adenosyl-L-homocysteine + N⁷-methylguanine⁴⁶ in tRNA
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 184.4)
  and 4 more
  Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 16.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 14.9)
  Genetic information processing Protein fate Protein modification and repair protein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 13.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 13.2)
• TheSEED  :
  • tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)
• PFAM:
  NADP_Rossmann (CL0063) Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 169.6)
  and 7 more
  DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 17.9)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 16.2)
  PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 15.4)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 15.2)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 13.3)
  Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 12.2)
  GidB; rRNA small subunit methyltransferase G (PF02527; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006715
  • TAT(Tat/SPI): 0.000454
  • LIPO(Sec/SPII): 0.000607
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160692 NCBI
• RefSeq: YP_494388 NCBI
• UniProt: Q2FFZ0 UniProt

⊟Protein sequence

• MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEIGSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMGLTNLKIICNDAIELNEYFKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAYSLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSDKGSRIYRMEAKFHSQK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: trmB < SAUSA300_1695

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications