PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1639 [new locus tag: SAUSA300_RS08940 ]
• pan locus tag^?: SAUPAN004317000
• symbol: phoP
• pan gene symbol^?: phoP
• synonym:
• product: alkaline phosphatase synthesis transcriptional regulatory protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1639 [new locus tag: SAUSA300_RS08940 ]
• symbol: phoP
• product: alkaline phosphatase synthesis transcriptional regulatory protein
• replicon: chromosome
• strand: -
• coordinates: 1797197..1797901
• length: 705
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914279 NCBI
• RefSeq: YP_494334 NCBI
• BioCyc: see SAUSA300_RS08940
• MicrobesOnline: 1293154 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCGCAAAAAGTGTTGGTAGTAGATGACGAACATTCAATCGTAACATTACTTAAATAT
  AACTTAGAAACAGCTGGTTATGAAGTTGTTGTCGCATTTGATGGTGATGAGGCTTTAGAA
  AAGGTAGAAAGTGAACAGCCAGATTTAATTATTTTAGATGTTATGCTACCTAAAAAAGAT
  GGCATTGACGTATGTAAGACTGTAAGAACAAATAAAAATTTAGTCCCTATATTAATGTTA
  ACGGCGAAAAATGATGAATTTGATCGGGTATTAGGTTTAGAATTAGGTGCCGATGACTAT
  ATGACTAAGCCTTTTTCACCTAGAGAAGTTGTTGCCCGTGTGAAAGCCATTTTAAGACGT
  TCTCAATTTGTAAAAGAAGTAGAAGAGATTGACGATGATATTACCATCGGATCGATACGT
  ATTAGACCCGATTATTTTGAAGTATATAAGCATAATGAGTTATTGGAGCTAACACCGAAA
  GAATTTGAACTCTTGCTTTATTTGATAGAAAGACAAGGCAGAGTCATTACGAGAGAGCAT
  ATGTTAAATACGGTATGGAATTATGAATTTGCAGGAGACTCAAGAATTGTTGATGTACAT
  ATCAGTCATTTAAGAGATAAATTAGAAGATAATCCTAAGAAGCCACAACTTATTAAAACT
  GTGCGTGGCTTAGGCTATAAATTGGAAAGACCTAAAGAACAATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_1639 [new locus tag: SAUSA300_RS08940 ]
• symbol: PhoP
• description: alkaline phosphatase synthesis transcriptional regulatory protein
• length: 234
• theoretical pI: 4.90242
• theoretical MW: 27122.1
• GRAVY: -0.345299

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 241.6)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 241.6)
  and 8 more
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 191.2)
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 138.6)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 76.9)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 76.9)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 76.9)
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 64.1)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 44.3)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 38.6)
• TheSEED  :
  • Alkaline phosphatase synthesis transcriptional regulatory protein PhoP
  Phosphorus Metabolism Phosphorus Metabolism - no subcategory High affinity phosphate transporter and control of PHO regulon  Alkaline phosphatase synthesis transcriptional regulatory protein PhoP
  and 1 more
  Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Alkaline phosphatase synthesis transcriptional regulatory protein PhoP
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 106.2)
  HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 104)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001974
  • TAT(Tat/SPI): 0.000101
  • LIPO(Sec/SPII): 0.000244
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162022 NCBI
• RefSeq: YP_494334 NCBI
• UniProt: A0A0H2XI00 UniProt

⊟Protein sequence

• MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVESEQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMTKPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPKEFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLIKTVRGLGYKLERPKEQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: phoR < phoP

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications