PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1598 [new locus tag: SAUSA300_RS08705 ]
• pan locus tag^?: SAUPAN004248000
• symbol: ruvA
• pan gene symbol^?: ruvA
• synonym:
• product: Holliday junction DNA helicase RuvA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1598 [new locus tag: SAUSA300_RS08705 ]
• symbol: ruvA
• product: Holliday junction DNA helicase RuvA
• replicon: chromosome
• strand: -
• coordinates: 1752637..1753239
• length: 603
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913116 NCBI
• RefSeq: YP_494293 NCBI
• BioCyc: see SAUSA300_RS08705
• MicrobesOnline: 1293113 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  
  ATGTACGCGTATGTCAAAGGTAAGTTAACACATTTATATCCTACACACGTAGTTGTTGAA
  ACTGCTGGTGTTGGTTATGAAATTCAAACACCAAATTCTTATCGTTTTCAAAAGCATCTA
  GATCATGAAGTTTTAATTCATACATCTTTAATTGTTCGTGAAGATGCACAATTATTGTAT
  GGATTTAGTAGTGAAGAAGAGAAAGATATGTTCTTGAGTTTAATTAAAGTTACTGGTATT
  GGTCCGAAATCAGCTTTAGCTATTTTAGCGACAAGTACGCCTAATGAAGTAAAACGTGCC
  ATTGAAAATGAAAATGATACGTATTTAACTAAATTCCCAGGAATTGGTAAGAAAACGGCA
  AGACAGATTGTCTTAGATTTAAAAGGTAAAGTGAAAATTACTGAAGAAGATAGCGATTCA
  TTATTACAAGTAGACGCTACTTCGACGGTGCAAGATCAATTCGTGCAAGAAGCAATGTTA
  GCGTTAGAAGCATTAGGTTATTCTAAACGAGAGCTTGCAAAAGTTGAGAAAACGTTAAAT
  AAAAATAAATATGACTCAGTTGATGAAGCTGTTAAGGCAGGTCTTCAATTAGTTGTATCT
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  603
  

⊟Protein

⊟General

• locus tag: SAUSA300_1598 [new locus tag: SAUSA300_RS08705 ]
• symbol: RuvA
• description: Holliday junction DNA helicase RuvA
• length: 200
• theoretical pI: 6.03453
• theoretical MW: 22262.3
• GRAVY: -0.246

⊟Function

• reaction:
  EC 3.6.4.12?  ExPASy
  DNA helicase ATP + H₂O = ADP + phosphate
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 174)
  and 3 more
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS13 (TIGR03631; HMM-score: 16.1)
  Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 13.8)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair recombination protein RecR (TIGR00615; HMM-score: 12.3)
• TheSEED  :
  • Holliday junction DNA helicase RuvA
  DNA Metabolism DNA recombination RuvABC plus a hypothetical  Holliday junction DNA helicase RuvA
  and 1 more
  DNA Metabolism DNA replication DNA-replication  Holliday junction DNA helicase RuvA
• PFAM:
  OB (CL0021) RuvA_N; RuvA N terminal domain (PF01330; HMM-score: 73.2)
  and 11 more
  HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 55)
  UBA (CL0214) RuvA_C; RuvA, C-terminal domain (PF07499; HMM-score: 39.1)
  HHH (CL0198) HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 27.5)
  IMS_HHH; IMS family HHH motif (PF11798; HMM-score: 23.6)
  H2TH (CL0303) Ribosomal_S13; Ribosomal protein S13/S18 (PF00416; HMM-score: 20.6)
  HHH (CL0198) HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 17.8)
  HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 16.2)
  5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 13.3)
  HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 13.3)
  HHH_4; Helix-hairpin-helix containing domain (PF14490; HMM-score: 12.9)
  DNA_pol_lambd_f; Fingers domain of DNA polymerase lambda (PF10391; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003512
  • TAT(Tat/SPI): 0.000142
  • LIPO(Sec/SPII): 0.001493
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160146 NCBI
• RefSeq: YP_494293 NCBI
• UniProt: Q2FG85 UniProt

⊟Protein sequence

• MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKHLDHEVLIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIENENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAMLALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: yajC < tgt < queA < ruvB < ruvA < SAUSA300_1599 < obgE

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications