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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1539 [new locus tag: SAUSA300_RS08390 ]
  • pan locus tag?: SAUPAN004162000
  • symbol: dnaJ
  • pan gene symbol?: dnaJ
  • synonym:
  • product: chaperone protein DnaJ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1539 [new locus tag: SAUSA300_RS08390 ]
  • symbol: dnaJ
  • product: chaperone protein DnaJ
  • replicon: chromosome
  • strand: -
  • coordinates: 1688136..1689275
  • length: 1140
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    GTGGCCAAAAGAGATTATTATGAAGTTTTAGGCATTAGTAAGGATGCTTCAAAAGATGAA
    ATCAAAAAAGCGTATCGAAAGCTTTCGAAAAAATATCATCCAGATATTAACAAAGAAGAA
    GGTGCAGATGAAAAGTTTAAAGAAATTTCTGAAGCCTATGAAGTTTTAAGTGATGATAAT
    AAACGCGCAAGCTATGATCAATTTGGACATGATGGTCCACAAGGTTTTGGTGGACAAGGA
    TTCAATGGCTCTGACTTTGGCGGTTTTAGTGGCTTCGGTGGCGGCGGTTTTGAAGATATC
    TTTAGCTCTTTCTTCGGTGGCGGCAGACAAAGAGATCCAAATGCACCGCAAAAAGGTGAT
    GATCTTCAGTATACAATGACATTAACATTTGAAGAAGCGGTATTTGGTACAACAAAAGAA
    ATTTCAATCCGTAAAGATGTAACATGCGAAACATGTCATGGTGATGGTGCAAAGCCTGGA
    ACAAGTAAAAAGACTTGTAGTTACTGTAATGGCGCTGGTCATGTAGCTGTAGAACAAAAC
    ACAATTTTAGGTAGAGTTCGTACTGAACAAGTTTGTCCTAAATGTAATGGAAGTGGTCAA
    GAATTTGAAGAAGCTTGTCCAACATGTCACGGAAAAGGTACTGAAAATAAAACAGTTAAA
    TTAGAAGTGAAAGTACCTGAAGGTGTCGACAATGAACAACAAATTAGATTAGCTGGTGAA
    GGCTCTCCAGGCGTGAATGGAGGACCTGCTGGTGATTTATACGTAGTATTTAGAGTTAAA
    CCATCTGAAACATTTAAACGTGATGGTGATGATATTTATTACAAATTAAATGTTAGCTTC
    CCACAAGCAGCTTTAGGCGATGAAATAAAAATACCAACATTAAATAATGAAGTTATGTTA
    ACAATCCCTGCTGGTACACAAACAGGTAAGCAATTCCGCTTAAAAGAAAAGGGTATAAAA
    AATGTTCATGGCTATGGATACGGAGATTTATATGTTGATATTAAAGTAGTAACGCCTACT
    AAATTAACAGATAGACAAAAAGAACTAATGAAAGAATTTGCCCAATTAAATGGTGAAGAA
    ATTAATGAACAACCTTCGAATTTTAAAGATAGAGCAAAAAGATTCTTTAAGGGAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1539 [new locus tag: SAUSA300_RS08390 ]
  • symbol: DnaJ
  • description: chaperone protein DnaJ
  • length: 379
  • theoretical pI: 5.79
  • theoretical MW: 41760.2
  • GRAVY: -0.783641

⊟Function[edit | edit source]

  • TIGRFAM:
    chaperone protein DnaJ (TIGR02349; HMM-score: 466)
    and 4 more
    Cellular processes Cellular processes Chemotaxis and motility terminal organelle assembly protein TopJ (TIGR03835; HMM-score: 157)
    Genetic information processing Protein fate Protein folding and stabilization Fe-S protein assembly co-chaperone HscB (TIGR00714; HMM-score: 21.4)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions uncharacterized phage protein (TIGR02642; HMM-score: 14.5)
    Genetic information processing Protein fate Protein modification and repair hydrogenase nickel insertion protein HypA (TIGR00100; HMM-score: 5.7)
  • TheSEED  :
    • Chaperone protein DnaJ
    Protein Metabolism Protein folding Protein chaperones  Chaperone protein DnaJ
    and 1 more
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Chaperone protein DnaJ
  • PFAM:
    no clan defined DnaJ_C; DnaJ C terminal domain (PF01556; HMM-score: 151.9)
    and 17 more
    Chaperone-J (CL0392) DnaJ; DnaJ domain (PF00226; HMM-score: 97.3)
    no clan defined DnaJ_CXXCXGXG; DnaJ central domain (PF00684; HMM-score: 51.2)
    C2H2-zf (CL0361) zf-C2H2_10; C2H2 zinc-finger (PF16588; HMM-score: 20.2)
    no clan defined Anti-TRAP; Tryptophan RNA-binding attenuator protein inhibitory protein (PF15777; HMM-score: 19)
    Cytochrome-c (CL0318) CCP_MauG; Di-haem cytochrome c peroxidase (PF03150; HMM-score: 13.9)
    Multiheme_cytos (CL0317) Cytochrome_C7; Cytochrome c7 and related cytochrome c (PF14522; HMM-score: 13.7)
    no clan defined DUF3365; Protein of unknown function (DUF3365) (PF11845; HMM-score: 12.9)
    Multiheme_cytos (CL0317) Cytochrome_C554; Cytochrome c554 and c-prime (PF13435; HMM-score: 12)
    Cytochrom_c3_2; Cytochrome c3 (PF14537; HMM-score: 11.9)
    no clan defined DUF5351; Family of unknown function (DUF5351) (PF17302; HMM-score: 11.9)
    Cytochrome-c (CL0318) Cytochrome_CBB3; Cytochrome C oxidase, cbb3-type, subunit III (PF13442; HMM-score: 10.9)
    RING (CL0229) zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger) (PF13920; HMM-score: 10.4)
    no clan defined HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 9.2)
    Cytochrome-c (CL0318) DUF1924; Domain of unknown function (DUF1924) (PF09086; HMM-score: 9.2)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 9)
    RING (CL0229) zf-RING_10; zinc RING finger of MSL2 (PF16685; HMM-score: 6.9)
    no clan defined zf-CHY; CHY zinc finger (PF05495; HMM-score: 6.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007689
    • TAT(Tat/SPI): 0.001249
    • LIPO(Sec/SPII): 0.00262
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADEKFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFSSFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPGTSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKTVKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKLNVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDIKVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HrcA (repression) regulon
    HrcA(TF)important in Heat shock response; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Arnaud Chastanet, Juliette Fert, Tarek Msadek
    Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria.
    Mol Microbiol: 2003, 47(4);1061-73
    [PubMed:12581359] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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