PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1460 [new locus tag: SAUSA300_RS07975 ]
• pan locus tag^?: SAUPAN004064000
• symbol: SAUSA300_1460
• pan gene symbol^?: —
• synonym:
• product: M20/M25/M40 family peptidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1460 [new locus tag: SAUSA300_RS07975 ]
• symbol: SAUSA300_1460
• product: M20/M25/M40 family peptidase
• replicon: chromosome
• strand: -
• coordinates: 1614593..1615726
• length: 1134
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914953 NCBI
• RefSeq: YP_494156 NCBI
• BioCyc: see SAUSA300_RS07975
• MicrobesOnline: 1292975 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATTAATGAGCAAAGATTACTTAATACGTTTTTTGAACTTGTACAAATCGATTCTGAG
  ACAGGGAATGAATCAACAATCCAACCTATTTTAAAAGAAAAATTCATAGCATTAGGGTTA
  GATGTTAAAGAAGATGAAGCGGCTAAACATCCTAAATTAGGTGCCAATAATCTAGTGTGT
  ACAATGAATAGTACTATCGAAGAAGGCGAAGTACCTAAATTATATTTGACGAGCCATATG
  GATACTGTTGTACCCGCAATTAATGTAAAGCCAATTGTAAAAGATGACGGCTACATATAC
  TCTGATGGTACGACTATTCTAGGTGCAGATGATAAAGCAGGATTAGCAGCGATGCTTGAA
  GTTTTGCAAGTGATAAAGGAACAACAAATCCCACATGGACAAATTCAATTTGTGATTACT
  GTTGGAGAAGAATCAGGTTTAATTGGTGCTAAAGAATTAAATTCAGAGTTGTTGGACGCG
  GACTTTGGTTATGCTATTGATGCAAGTGCTGATGTCGGTACTACGGTTGTAGGTGCACCG
  ACGCAAATGTTAATTTCAGCTAAAATTTTTGGCAAAACGGCTCATGCAAGTACGCCAAAA
  GAGGGTGTTAGTGCTATTAATATTGCGGCGAAAGCAATTAGTCGAATGAAATTAGGACAA
  GTTGATGAAATAACTACTGCCAATATTGGTAAGTTTCATGGCGGTTCAGCTACGAATATT
  GTTGCAGATGAAGTCATTTTAGAAGCAGAGGCACGTTCTCACGATCCAGAAAGAATAAAA
  ACACAAGTCAAACATATGACTGATGTGTTTGAAACTACTGCATCAGAATTAGGTGGGAAA
  GCAGTAGTTACAGTGGAGCAAAGTTATCCAGGATTTAAAATTAATGATAATGAAGCGGTT
  GTTAAAATTGCACAAGAAAGTGCTCGTAATTTAGGATTATCTGCAAATACAATTATTTCT
  GGTGGCGGTTCAGACGGCAGTATTATTAATACATTTGGTATTCCTTCAGTCATTTTAGGA
  GTGGGTTATGAAAAAATCCATACAACAAATGAGAGAATGCCGATTAAATCATTGAACTTG
  CTTGCAAGTCAAGTGTTAGAAATAATCAAAATAGTTGCGCGACATTCGAAATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_1460 [new locus tag: SAUSA300_RS07975 ]
• symbol: SAUSA300_1460
• description: M20/M25/M40 family peptidase
• length: 377
• theoretical pI: 4.79982
• theoretical MW: 40243.6
• GRAVY: -0.00928382

⊟Function

• TIGRFAM:
  peptidase T-like protein (TIGR01883; HMM-score: 402.7)
  and 14 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase, ArgE/DapE family (TIGR01910; EC 3.4.-.-; HMM-score: 114.4)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase T (TIGR01882; EC 3.4.11.-; HMM-score: 86.1)
  Metabolism Amino acid biosynthesis Glutamate family acetylornithine deacetylase (ArgE) (TIGR01892; EC 3.5.1.16; HMM-score: 84.7)
  Xaa-His dipeptidase (TIGR01893; EC 3.4.13.20; HMM-score: 78.1)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides amidohydrolase (TIGR01891; HMM-score: 77.8)
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01900; EC 3.5.1.18; HMM-score: 67.7)
  Metabolism Amino acid biosynthesis Aspartate family succinyl-diaminopimelate desuccinylase (TIGR01246; EC 3.5.1.18; HMM-score: 57.5)
  N-acetyl-ornithine/N-acetyl-lysine deacetylase (TIGR01902; HMM-score: 38.1)
  putative selenium metabolism hydrolase (TIGR03526; HMM-score: 38)
  amidase, hydantoinase/carbamoylase family (TIGR01879; EC 3.5.-.-; HMM-score: 26.2)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides dipeptidase PepV (TIGR01886; EC 3.4.13.-; HMM-score: 25.7)
  hydrolase, peptidase M42 family (TIGR03106; HMM-score: 23.4)
  putative dipeptidase (TIGR01887; EC 3.4.13.-; HMM-score: 21.2)
  Metabolism Amino acid biosynthesis Aromatic amino acid family chorismate mutase (TIGR01801; EC 5.4.99.5; HMM-score: 12.6)
• TheSEED  :
  • Peptidase T (EC 3.4.11.4)
• PFAM:
  Peptidase_MH (CL0035) Peptidase_M20; Peptidase family M20/M25/M40 (PF01546; HMM-score: 86.7)
  and 3 more
  Peptidase_M42; M42 glutamyl aminopeptidase (PF05343; HMM-score: 62.4)
  no clan defined M20_dimer; Peptidase dimerisation domain (PF07687; HMM-score: 49.5)
  Peptidase_MH (CL0035) Peptidase_M28; Peptidase family M28 (PF04389; HMM-score: 43.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: a divalent metal cation
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004919
  • TAT(Tat/SPI): 0.000424
  • LIPO(Sec/SPII): 0.000471
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159907 NCBI
• RefSeq: YP_494156 NCBI
• UniProt: A0A0H2XFJ9 UniProt

⊟Protein sequence

• MINEQRLLNTFFELVQIDSETGNESTIQPILKEKFIALGLDVKEDEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKPIVKDDGYIYSDGTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDADFGYAIDASADVGTTVVGAPTQMLISAKIFGKTAHASTPKEGVSAINIAAKAISRMKLGQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASELGGKAVVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGIPSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARHSK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: gnd < SAUSA300_1460

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications