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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1222 [new locus tag: SAUSA300_RS06625 ]
  • pan locus tag?: SAUPAN003694000
  • symbol: nuc
  • pan gene symbol?: nuc2
  • synonym:
  • product: thermonuclease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1222 [new locus tag: SAUSA300_RS06625 ]
  • symbol: nuc
  • product: thermonuclease
  • replicon: chromosome
  • strand: +
  • coordinates: 1339327..1339860
  • length: 534
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAGTCAAATAAATCGCTTGCTATGATTGTGGTAGCCATCATTATTGTAGGTGTATTA
    GCATTTCAATTTATGAATCATACGGGTCCTTTCAAAAAGGGGACGAATCATGAAACTGTA
    CAAGATTTAAATGGTAAAGATAAAGTACATGTTCAAAGAGTTGTGGATGGTGATACATTT
    ATTGCAAATCAAAATGGTAAAGAAATTAAAGTTAGGCTTATAGGGGTTGATACGCCAGAA
    ACGGTGAAACCGAATACGCCTGTACAACCATTTGGCAAAGAAGCATCAAATTATAGTAAG
    AAGACATTAACAAATCAAGATGTTTATTTAGAATATGATAAAGAAAAACAAGATCGCTAT
    GGTAGAACATTGGCGTATGTATGGATAAGTAAAGATCGTATGTACAATAAGGAATTAGTG
    GAAAAGGGACTTGCTAGAGAGAAGTATTTTTCACCAAATGGCAAATATAGAAATGTATTT
    ATAGAAGCACAAAATAAAGCTAAACAACAGAAATTAAATATTTGGAGTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    534

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1222 [new locus tag: SAUSA300_RS06625 ]
  • symbol: Nuc
  • description: thermonuclease
  • length: 177
  • theoretical pI: 10.2676
  • theoretical MW: 20328.2
  • GRAVY: -0.69096

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.31.1?  ExPASy
    Micrococcal nuclease Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
  • TIGRFAM:
  • TheSEED  :
    • Micrococcal nuclease (EC 3.1.31.1)
  • PFAM:
    Tudor (CL0049) SNase; Staphylococcal nuclease homologue (PF00565; HMM-score: 113.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.576138
    • TAT(Tat/SPI): 0.002296
    • LIPO(Sec/SPII): 0.041752
    • Cleavage Site: CS pos: 28-29. NHT-GP. Pr: 0.0688
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVFIEAQNKAKQQKLNIWSK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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