PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1086 [new locus tag: SAUSA300_RS05885 ]
• pan locus tag^?: SAUPAN003463000
• symbol: SAUSA300_1086
• pan gene symbol^?: divIVA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1086 [new locus tag: SAUSA300_RS05885 ]
• symbol: SAUSA300_1086
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1186239..1186856
• length: 618
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914026 NCBI
• RefSeq: YP_493783 NCBI
• BioCyc: see SAUSA300_RS05885
• MicrobesOnline: 1292601 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGCCTTTTACACCAAATGAAATTAAGAATAAAGAGTTTTCACGTGTAAAGAATGGTTTA
  GAACCTACTGAAGTTGCTAATTTTTTGGAGCAACTAAGCACTGAAATTGAACGTCTTAAA
  GAAGATAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTAT
  CAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAA
  ACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCT
  AATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTCCAGACTGAAGAT
  ATGAAACGTCAATCAAAAGTATTTAGATCGCGTTTCCGTATGTTAGTTGAAGCGCAATTA
  GACTTATTAAAAAACGAAGATTGGGATTACTTGTTGAATTATGATTTAGACGCTGAACAA
  GTGACGCTTGAAAATATTCATCATTTGCATGAAAATGATTTAAAGCCAGATGAAGTTGCA
  GCAAATGCACAAAATAATGCATCAAATACACCAGACAATAATCAACAATCCAATGATTCA
  GAAACAACTAAGAAGTAA
  60
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  618
  

⊟Protein

⊟General

• locus tag: SAUSA300_1086 [new locus tag: SAUSA300_RS05885 ]
• symbol: SAUSA300_1086
• description: hypothetical protein
• length: 205
• theoretical pI: 4.63557
• theoretical MW: 23513.8
• GRAVY: -1.00683

⊟Function

• TIGRFAM:
  DivIVA domain (TIGR03544; HMM-score: 29.9)
  and 5 more
  Genetic information processing DNA metabolism Other MutS2 family protein (TIGR01069; HMM-score: 12.7)
  Cellular processes Cellular processes Adaptations to atypical conditions phage shock protein B (TIGR02976; HMM-score: 10.8)
  Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F0, B subunit (TIGR01144; EC 3.6.3.14; HMM-score: 5.6)
  ATP synthase archaeal, H subunit (TIGR02926; EC 3.6.3.14; HMM-score: 4.9)
  SH3 domain protein (TIGR04211; HMM-score: 4.8)
• TheSEED  :
  • Cell division initiation protein DivIVA
  Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division initiation protein DivIVA
• PFAM:
  no clan defined DivIVA; DivIVA protein (PF05103; HMM-score: 119.1)
  and 19 more
  ATP_synthase (CL0255) ATP-synt_B; ATP synthase B/B' CF(0) (PF00430; HMM-score: 18.1)
  no clan defined Leu_zip; Leucine zipper (PF15294; HMM-score: 17.7)
  CDA (CL0109) APOBEC_N; APOBEC-like N-terminal domain (PF08210; HMM-score: 13.7)
  no clan defined DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 12.1)
  HAD (CL0137) 5_nucleotid; 5' nucleotidase family (PF05761; HMM-score: 12)
  no clan defined DUF2570; Protein of unknown function (DUF2570) (PF10828; HMM-score: 11.7)
  ISG65-75; Invariant surface glycoprotein (PF11727; HMM-score: 11.6)
  FlaC_arch; Flagella accessory protein C (FlaC) (PF05377; HMM-score: 10.9)
  KELK; KELK-motif containing domain of MRCK Ser/Thr protein kinase (PF15796; HMM-score: 10.6)
  Dak2; DAK2 domain (PF02734; HMM-score: 9.5)
  ZYG-11_interact; Interactor of ZYG-11 (PF05884; HMM-score: 9.5)
  CENP-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1 (PF10473; HMM-score: 9.1)
  DUF349; Domain of Unknown Function (DUF349) (PF03993; HMM-score: 8.7)
  YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 7.8)
  no clan defined YtxH; YtxH-like protein (PF12732; HMM-score: 7.6)
  NADP_Rossmann (CL0063) NAD_binding_5; Myo-inositol-1-phosphate synthase (PF07994; HMM-score: 7.3)
  no clan defined LMBR1; LMBR1-like membrane protein (PF04791; HMM-score: 6.6)
  TMPIT; TMPIT-like protein (PF07851; HMM-score: 6.2)
  DASH_Spc19; Spc19 (PF08287; HMM-score: 6.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002206
  • TAT(Tat/SPI): 0.000287
  • LIPO(Sec/SPII): 0.000323
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160546 NCBI
• RefSeq: YP_493783 NCBI
• UniProt: A0A0H2XER6 UniProt

⊟Protein sequence

• MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKKQLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQMVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQVTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1081 > SAUSA300_1082 > SAUSA300_1083 > SAUSA300_1084 > SAUSA300_1085 > SAUSA300_1086

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications