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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1049 [new locus tag: SAUSA300_RS05650 ]
  • pan locus tag?: SAUPAN003390000
  • symbol: murI
  • pan gene symbol?: murI
  • synonym:
  • product: glutamate racemase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1049 [new locus tag: SAUSA300_RS05650 ]
  • symbol: murI
  • product: glutamate racemase
  • replicon: chromosome
  • strand: +
  • coordinates: 1148276..1149076
  • length: 801
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATAAACCAATAGGTGTAATAGACTCTGGTGTCGGAGGTTTGACAGTAGCTAAAGAA
    ATTATGCGTCAGTTGCCAAATGAGACGATTTATTACTTAGGTGATATTGGACGATGTCCA
    TATGGGCCAAGACCAGGAGAACAAGTAAAACAATATACAGTTGAAATCGCTCGTAAATTA
    ATGGAATTTGATATAAAAATGCTCGTGATTGCTTGTAATACAGCAACTGCTGTAGCTTTA
    GAATATTTACAAAAGACCTTATCAATCCCAGTGATTGGCGTAATTGAACCAGGTGCTAGA
    ACAGCAATAATGACTACTAGAAATCAAAATGTATTAGTACTAGGAACGGAAGGCACAATT
    AAATCTGAAGCATATCGTACGCATATTAAACGTATCAATCCACATGTAGAGGTACATGGC
    GTTGCCTGTCCAGGTTTTGTGCCACTTGTAGAACAAATGAGATATAGTGATCCAACAATT
    ACAAGCATTGTCATTCATCAAACACTGAAACGTTGGCGTAATAGTGAGTCTGATACTGTC
    ATTTTAGGATGTACCCACTATCCATTGCTCTATAAACCTATCTATGATTATTTTGGTGGT
    AAAAAGACAGTGATTTCGTCTGGATTAGAAACGGCTCGTGAAGTTAGTGCATTGCTAACA
    TTTAGTAATGAACATGCAAGTTATACTGAACATCCAGATCATCGATTTTTTGCAACAGGT
    GATCCTACTCACATTACTAACATTATCAAAGAGTGGTTAAATTTATCTGTCAATGTGGAA
    CGTATATCAGTGAATGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    801

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1049 [new locus tag: SAUSA300_RS05650 ]
  • symbol: MurI
  • description: glutamate racemase
  • length: 266
  • theoretical pI: 7.63246
  • theoretical MW: 29698
  • GRAVY: -0.120301

⊟Function[edit | edit source]

  • reaction:
    EC 5.1.1.3?  ExPASy
    Glutamate racemase L-glutamate = D-glutamate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamate racemase (TIGR00067; EC 5.1.1.3; HMM-score: 271.7)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines aspartate racemase (TIGR00035; EC 5.1.1.13; HMM-score: 51.1)
    Cellular processes Cellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 12.9)
  • TheSEED  :
    • Glutamate racemase (EC 5.1.1.3)
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis  Glutamate racemase (EC 5.1.1.3)
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  Glutamate racemase (EC 5.1.1.3)
  • PFAM:
    Asp-glut_race (CL0399) Asp_Glu_race; Asp/Glu/Hydantoin racemase (PF01177; HMM-score: 117.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013508
    • TAT(Tat/SPI): 0.000517
    • LIPO(Sec/SPII): 0.003397
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPRPGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTAIMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTITSIVIHQTLKRWRNSESDTVILGCTHYPLLYKPIYDYFGGKKTVISSGLETAREVSALLTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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